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transfections in Biological News

Yamanaka eliminates viral vector in stem cell reprogramming

... reveals a new set of challenges." Dr. Yamanka's team began this series of experiments by replacing the retrovirus with an adenoviral vector. While transfections with the genes on separate vectors didn't work, they did work when the genes were arranged in a specific order on a single vector. The same arrangemen...
transfections in Biological Technology

High-Efficiency Transfections Achieved with New Low-Toxicity Reagent

Nonliposomal formulation yields high efficiencies in a variety of transfection conditions Brenda Rogers Paul Kotturi Natalia Novoradovskaya Stratagene Introducing foreign DNA into eukaryotic cells requires high-potency transfections, which demand superior quality transfec...

Streamline Your siRNA Transfections

NEW siPORT NeoFX Transfection Agent Promotes High Reproducibility and Efficiency Fast--transfect cells as they are plated (reverse transfection), saving a day ...

Clontech Laboratories, Inc. Releases the Xfect(TM) and Xfect(TM) Stem Transfection Reagents

...ents are based on biodegradable nanoparticles that permit superior transfection efficiency of plasmid DNA into mammalian cells. For added ease of use, transfections can be carried out entirely in the presence of serum. Xfect shows high efficiency over a broad range of cell types, while Xfect Stem meets the highly ...

FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

...ES SUB-OPTIMAL ASSAYS IMPROVED FUNCTIONALITY IN TRANSIENT transfections ABSTRACT FLIPR Calcium 3 Assay Kit (Calcium 3), the... no-wash reagent to measure agonist-stimulated calcium signaling. Transient transfections are frequently used prior to generation of stable cell lines to confirm fun...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

... INTRODUCTION Reporter gene assays are used to study eukaryotic gene expression. Dual geneticreporters are commonly used in transient transfections of cultured cells tominimize experimental variability caused by differences in cell number, viabilityor transfection efficiency. One vector containing...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

... enzymes to establish DNA purity. transfections Three cell lines, RR1, VERO and NIH3T...plates the day before transfection. All transfections were perf o rmed in triplicate. For eac... results, shown in the table, demonstrate that transfections with pHSVlac prepared using method D re...

Your Data: Silencing Neural Cell Adhesion Genes with In Vitro Transcribed siRNAs

...stern blots 96-hours post-transfection. (B) Gene 2 expression. transfections were carried out as described in panel A. (C) -actin expression (internal control). transfections were carried out as described in panel A. ...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

...carrier. To address this question, we repeated our transfections using an NBD-labeled carrier lipid ...r the manufacturer's protocol. transfections HeLa S3 cells were plated at 50,000 cells/well...ntaining glass cover slips (11 mm). transfections were performed 24 hr after plating using

Setting up Successful siRNA Library Screens

...bion.com/techlib/tn/116/19.html">Streamline Your siRNA transfections ). (3) Screen Once the...tially into cells in a 24 or 96 well plate. Triplicate transfections for each siRNA provide enough data for reasonable stat...

Recommendations for Successful siRNA Library Screens

... Although many siRNA transfections are still performed with 100 nM siRNA, ... should analyze the combined results from separate transfections of three different gene-specific siRNAs... be feasible, results from single siRNA transfections of one (#1, #2, #3) or two (#1+#2, #1+#3, #2+#3) g...
transfections in Biological Products

BD BaculoGoldTM Transfection Kit 5 transfections from BD Biosciences Pharmingen

Description: BD BaculoGoldTM Transfection Kit 5 transfections...
Company:BD Biosciences Pharmingen

BacPlaque Agarose from Novagen

Description:... insect cells or lack the physical characteristics required to obtain good overlays. Novagen's BacPlaque Agarose is pre-qualified in plaque assays and transfections to ensure consistent performance....
Company:Novagen

TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation

Description:...les highly efficient siRNA transfection with significantly reduced levels of cell damage as compared to cationic-liposome based transfection reagents. transfections are most effective when carried out in complete growth media, with no media change or serum addition required. TransIT-siQUEST Reagent efficiently del...
Company:Mirus Bio Corporation

TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation

Description:...les highly efficient siRNA transfection with significantly reduced levels of cell damage as compared to cationic-liposome based transfection reagents. transfections are most effective when carried out in complete growth media, with no media change or serum addition required. TransIT-siQUEST Reagent efficiently del...
Company:Mirus Bio Corporation

Label IT siRNA Tracker Biotin Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation

Description:...ovides sufficient reagents to label 50 mg of siRNA and perform at least 500 transfections in 24-well plates. TransIT-TKO Transfection Reagent, which is included in the kit, is designed specifically for high efficiency, low toxicity siRNA transfections in a variety of cell lines....
Company:Mirus Bio Corporation

Label IT siRNA Tracker Cy3 Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation

Description:...ollowing introduction of the labeled siRNA into mammalian cells. This kit provides sufficient reagents to label 50 g of siRNA and perform at least 500 transfections in 24-well plates. TransIT-siQUEST Transfection Reagent, which is included in the kit, is designed specifically for the high efficiency, low toxicity ...
Company:Mirus Bio Corporation

Label IT siRNA Tracker Biotin Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation

Description:...ollowing introduction of the labeled siRNA into mammalian cells. This kit provides sufficient reagents to label 50 g of siRNA and perform at least 500 transfections in 24-well plates. TransIT-siQUEST Transfection Reagent, which is included in the kit, is designed specifically for the high efficiency, low toxicity ...
Company:Mirus Bio Corporation

Label IT siRNA Tracker TM Rhodamine Kit with TransIT-siQUEST Reagent from Mirus Bio Corporation

Description:...ollowing introduction of the labeled siRNA into mammalian cells. This kit provides sufficient reagents to label 50 g of siRNA and perform at least 500 transfections in 24-well plates. TransIT-siQUEST Transfection Reagent, which is included in the kit, is designed specifically for the high efficiency, low toxicity ...
Company:Mirus Bio Corporation

Label IT siRNA Tracker Fluorescein Intracellular Localization Kit with TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation

Description:...ovides sufficient reagents to label 50 mg of siRNA and perform at least 500 transfections in 24-well plates. TransIT-TKO Transfection Reagent, which is included in the kit, is designed specifically for high efficiency, low toxicity siRNA transfections in a variety of cell lines....
Company:Mirus Bio Corporation

Label IT siRNA Tracker Intracellular Localization Kit with TransIT-TKO Transfection Reagent (Cy5 Labeling Kit) from Mirus Bio Corporation

Description:...ovides sufficient reagents to label 50 mg of siRNA and perform at least 500 transfections in 24-well plates. TransIT-TKO Transfection Reagent, which is included in the kit, is designed specifically for high efficiency, low toxicity siRNA transfections in a variety of cell lines. Each Kit contains Label IT siRNA Tracker Reage...
Company:Mirus Bio Corporation
transfections in Biological Dictionary

Stable transfection

... Transfection ... Major applications for stable transfection are the analysis of gene function and ... Nucleofector Technology and Stable transfections ... Home > Applications > Stable Transfection > Technology. RNAi. Protein Production ... Methods, protocols, techniques, tutorials and...
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