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transcriptases in Biological News

Biochemists report discovery of structure of major piece of telomerase; implications for cancer

...igon's laboratory has been working on the RNA. "It is a dream of mine to figure out what this RNA is doing with the protein," Feigon said. "Reverse transcriptases normally copy RNA to DNA, but do not contain RNA; in this enzyme, the protein requires the RNA component to function. The enzyme is unique because it ...
transcriptases in Biological Technology

Reverse transcriptases suitable for two-step RT-PCRd

Product size Type of primers Primer anneal. temp. Sensitivity e Specificity f Fidelity in RT GC-rich structures ...

Reverse transcriptases suitable for one-step RT-PCRb

Product size Type of primers Primer anneal. temp. Sensitivity Specifi- city Fidelity in PCR Tolerance ...

Properties of Reverse Transcriptases

Enzyme Maximum size of template Temperature optimum Units needed for standard reaction Ion require- ment Rnase H activity ...

Suggested applications for reverse transcriptases

Enzyme Full-Length cDNA High fidelity cDNA for cloning Amplification of viral RNA DDDRT a RACE a ...

Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

...iological activity. These assays measure the inhibitory effect of test compounds on target enzymes such as HIV proteases, various kinases, RNA and DNA transcriptases and polymerases. Enzyme activity is often determined by measuring the conversion of a radiolabeled substrate to a product that can be separated from t...

RNA Amplification Just Got Better: MessageAmp II

... yields of full-length cDNA compared to other reverse transcriptases (Figure 1). Figure 2 demonstrates that up to twice as ...ild type M-MLV, AMV, or other engineered M-MLV reverse transcriptases when low amounts of total RNA are used (~100 ng). This...

HIGH SENSITIVITY qRT-PCR

...ore sensitive than SuperScript II and other RNase H MMLV reverse transcriptases Linear quantitation of mRNA targets over a 106-fol...sitivity in RT-PCR RNase H minus (RNase H ) reverse transcriptases (RTs) are a popular choice among researchers for appli...

Better RNA Amplification

...ll-length, double-stranded cDNA templates. ArrayScript produces the highest yields of full-length cDNA compared to other reverse transcriptases (Figure 1). The second-strand cDNA synthesis reaction has been optimized for maximal conversion of first-strand cDNA into full-l...

ArrayScript RT: Maximize Yields of Full-length cDNA

...ed using wild type M-MLV, AMV, or other engineered M-MLV reverse transcriptases (Figure 2). When starting with low amounts of total RNA, ArraySc...or all RT Applications Reverse transcriptases are also used in RT-PCR, cDNA library construction, an...

Choice of RT-PCR Enzymes

...lse priming. Thus, thermoactive reverse transcriptases that can be incubated at high temperatures (5070C)...rast to Tth DNA Polymerase, AMV and M-MuLV Reverse transcriptases have RNase H activity. This activity wi...nt ion requirement Most reverse transcriptases require a divalent ion for activity. Enzy...
transcriptases in Biological Dictionary

RT-PCR

...te as a ... Amplifying RNA with RT - PCR . Overview. The use of reverse transcriptases to perform first-strand ... are one-tube, one-step, coupled RT - PCR Syste... RT - PCR System saves you four pipetting steps ... cDNA Synthesis Reverse transcriptases Reverse Transcription Systems RT - PCR RNase Inhibitors ... Methods, p...

Reverse transcription

...erse Transcription System, visit: ... The ImProm-II™ Reverse Transcription System is excellent for reproducible ... cDNA Synthesis Reverse transcriptases Reverse Transcription Systems RT-PCR RNase ... ied the reverse transcription reaction and its conse ... and gene-specific reverse transc...

Group I introns

...mains I and IV. ... Group II introns are self-splicing ribozymes believed to be the ancestors of ... Many group II introns encode reverse transcriptases that promote both RNA ... Group ,II, introns ,biological ,biology dictionary,biology terminology,biology terms,biology abbreviations ... Not all ...

Group II introns

...ice without ... Group II introns are self-splicing ribozymes believed to be the ancestors of ... Many group II introns encode reverse transcriptases that promote both RNA ... Global Regulators Orchestrate Group II Intron Retromobility ... Retrohoming: cDNA-Mediated Mobility of Group II ...
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