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sonicated in Medical News

Helicobacter pylori inhibits intercellular communication of cultured gastric cells

...ted gastric cancer has been little exploited. In this article, the researchers treated a human gastric cell line in vitro with intact bacteria and sonicated extracts of two H pylori strains with the virulence protein CagA. These were positive (CagA+) and negative (CagA-), respectively. After overnight trea...
sonicated in Biological Technology

Transformation of Filamentous Fungi by Microprojectile Bombardment

...tes were incubated for 68 days at 37 C at which time conidia were harvested in 0.9% sterile saline. The resuspended conidia were sonicated in an ultra sound sonicator for 1 min prior to being used for protoplast formation or for microprojectile bombardment. ...

Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment

... MICROCARRIERS Fifty mg gold microcarriers (diameter: 0.6 or 1.0 m) and 100 l of 0.05 M spermidine were vortexed for 5 sec and sonicated for 10 sec in an ultrasonic bath to break up gold clumps. To obtain a DNA Loading Ratio (DLR) of 2, 100 g plasmid DNA in 100 l d...

Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C

..., containing 5 g/ml DNase I and 1 mg/ml lysozyme, and incubated for 10 min at room temperature. Subsequently, the suspension was sonicated on ice (150 W, 5 x 30 sec) and centrifuged at 4,000 x g for 10 min, and aliquots were analyzed by SDS-PAGE to monitor expression...

Rapid hit confirmation and early hERG liability testing using the IonWorks HT system

... and stored at 4C. Prior to cell preparation, 180 l DMSO was added to an aliquot of amphotericin. Amphotericin/DMSO solution was sonicated until soluble (~1 minute), added to a 50 mL conical tube of Internal Buffer and vortexed for ~1 minute. The solution was stored ...

Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer

... in cold PBS, pelleted, re-suspended to 2 mL in PBS and frozen at 80C. The cell suspension was thawed, diluted to 5 mL in cold PBS, and sonicated for 30 seconds followed by ultracentrifugation at 100,000 x g for 1 hour. The supernatant was removed and the pellet resuspended in...

HotStarTaq PCR Handbook

...label inside kit lid for lot-specific values.) Unit assay: sonicated herring-sperm DNA (12.5 g) is incubated with 0.010.1 units of HotStarTaq DNA Polymerase in assay buffer (25 ...
sonicated in Biological Products

Leukocytes, human - sonicated from AppliChem GmbH

Description: NOTE: 0.9 ml per liter of urine will give a positive reaction for leukocytes on an Ames or BMD dipstick. Certification: The Donors are of US origin and have been tested and found to be NEGATIVE for HbsAg, HCV, HIV-1 & 2, Syphilis and HIV-1 Antigen by currently approved FDA methods. THIS MATERIAL ...
Company:AppliChem GmbH

Dog Anti-Pig Pancreatic Islets of Langerhans Cells Polyclonal Antibody, Unconjugated from Abcam

Description:...g polyclonal to Porcine Pancreatic Islets of Langerhans Cells ( Abpromise for all tested applications). Antigen: Tissue / cell preparation (Pig): sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells....
Company:Abcam

Benzonase from Sigma-Aldrich

Description:...sical form: Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl 2 , and 20 mM NaCl Preparation Unit Definition: One unit will digest sonicated herring sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA 260 of 1.0 in 30 min at pH 8.0 at 37 C (reaction volume 2.625 ml). Assay: ...
Company:Sigma-Aldrich
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