Transformation of Filamentous Fungi by Microprojectile Bombardment
...tes were incubated for 68 days
at 37 C at which time conidia were harvested in 0.9% sterile saline.
The resuspended conidia were sonicated
in an ultra sound sonicator for
1 min prior to being used for protoplast formation or for microprojectile
Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Fifty mg gold microcarriers (diameter: 0.6 or 1.0 m) and 100 l of 0.05
M spermidine were vortexed for 5 sec and sonicated
for 10 sec in an ultrasonic
bath to break up gold clumps. To obtain a DNA Loading Ratio (DLR) of 2,
100 g plasmid DNA in 100 l d...
Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C
..., containing 5 g/ml DNase I and 1
mg/ml lysozyme, and incubated for 10 min at room temperature. Subsequently,
the suspension was sonicated
on ice (150 W, 5 x 30 sec) and centrifuged
at 4,000 x g for 10 min, and aliquots were analyzed by SDS-PAGE to monitor
Rapid hit confirmation and early hERG liability testing using the IonWorks HT system
... and stored at 4C.
Prior to cell preparation, 180 l DMSO was added to an aliquot of
amphotericin. Amphotericin/DMSO solution was sonicated
until soluble (~1
minute), added to a 50 mL conical tube of Internal Buffer and vortexed
for ~1 minute. The solution was stored ...
Quantification of the Purinergic Receptor P2X3 using ICAT and
Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer
in cold PBS, pelleted, re-suspended to 2 mL in PBS and frozen at 80C.
The cell suspension was thawed, diluted to 5 mL in cold PBS, and sonicated
for 30 seconds followed by ultracentrifugation at 100,000 x g
for 1 hour. The supernatant was removed and the pellet resuspended in...
HotStarTaq PCR Handbook
...label inside kit lid for
herring-sperm DNA (12.5 g) is
incubated with 0.010.1 units of HotStarTaq DNA Polymerase
in assay buffer (25 ...
Leukocytes, human - sonicated from AppliChem GmbH
Description: NOTE: 0.9 ml per liter of urine will give a positive reaction for leukocytes on an Ames or BMD dipstick.
Certification: The Donors are of US origin and have been tested and found to be NEGATIVE for HbsAg, HCV, HIV-1 & 2, Syphilis and HIV-1 Antigen by currently approved FDA methods. THIS MATERIAL ...
Dog Anti-Pig Pancreatic Islets of Langerhans Cells Polyclonal Antibody, Unconjugated from Abcam
Description:...g polyclonal to Porcine Pancreatic Islets of Langerhans Cells ( Abpromise for all tested applications). Antigen: Tissue / cell preparation (Pig): sonicated
crude membrane fraction of porcine pancreatic islets of langerhans cells....
Benzonase from Sigma-Aldrich
Description:...sical form: Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl 2 , and 20 mM NaCl Preparation Unit Definition: One unit will digest sonicated
herring sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA 260 of 1.0 in 30 min at pH 8.0 at 37 C (reaction volume 2.625 ml). Assay: ...