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sonicated in Medical News

Helicobacter pylori inhibits intercellular communication of cultured gastric cells

...ted gastric cancer has been little exploited. In this article, the researchers treated a human gastric cell line in vitro with intact bacteria and sonicated extracts of two H pylori strains with the virulence protein CagA. These were positive (CagA+) and negative (CagA-), respectively. After overnight trea...
sonicated in Biological Technology

Transformation of Filamentous Fungi by Microprojectile Bombardment

...tes were incubated for 68 days at 37 C at which time conidia were harvested in 0.9% sterile saline. The resuspended conidia were sonicated in an ultra sound sonicator for 1 min prior to being used for protoplast formation or for microprojectile bombardment. ...

Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment

... MICROCARRIERS Fifty mg gold microcarriers (diameter: 0.6 or 1.0 m) and 100 l of 0.05 M spermidine were vortexed for 5 sec and sonicated for 10 sec in an ultrasonic bath to break up gold clumps. To obtain a DNA Loading Ratio (DLR) of 2, 100 g plasmid DNA in 100 l d...

Preparative SDS-PAGE Electrophoresis of a Recombinant Epstein-Barr Virus Encoded Protein and Its Application in Serodiagnostic Test Systems, Rev C

..., containing 5 g/ml DNase I and 1 mg/ml lysozyme, and incubated for 10 min at room temperature. Subsequently, the suspension was sonicated on ice (150 W, 5 x 30 sec) and centrifuged at 4,000 x g for 10 min, and aliquots were analyzed by SDS-PAGE to monitor expression...

Rapid hit confirmation and early hERG liability testing using the IonWorks HT system

... and stored at 4C. Prior to cell preparation, 180 l DMSO was added to an aliquot of amphotericin. Amphotericin/DMSO solution was sonicated until soluble (~1 minute), added to a 50 mL conical tube of Internal Buffer and vortexed for ~1 minute. The solution was stored ...

Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer

... in cold PBS, pelleted, re-suspended to 2 mL in PBS and frozen at 80C. The cell suspension was thawed, diluted to 5 mL in cold PBS, and sonicated for 30 seconds followed by ultracentrifugation at 100,000 x g for 1 hour. The supernatant was removed and the pellet resuspended in...

HotStarTaq PCR Handbook

...label inside kit lid for lot-specific values.) Unit assay: sonicated herring-sperm DNA (12.5 g) is incubated with 0.010.1 units of HotStarTaq DNA Polymerase in assay buffer (25 ...
sonicated in Biological Products

Leukocytes, human - sonicated from AppliChem GmbH

Description: NOTE: 0.9 ml per liter of urine will give a positive reaction for leukocytes on an Ames or BMD dipstick. Certification: The Donors are of US origin and have been tested and found to be NEGATIVE for HbsAg, HCV, HIV-1 & 2, Syphilis and HIV-1 Antigen by currently approved FDA methods. THIS MATERIAL ...
Company:AppliChem GmbH

Dog Anti-Pig Pancreatic Islets of Langerhans Cells Polyclonal Antibody, Unconjugated from Abcam

Description:...g polyclonal to Porcine Pancreatic Islets of Langerhans Cells ( Abpromise for all tested applications). Antigen: Tissue / cell preparation (Pig): sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells....
Company:Abcam

Benzonase from Sigma-Aldrich

Description:...sical form: Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl 2 , and 20 mM NaCl Preparation Unit Definition: One unit will digest sonicated herring sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA 260 of 1.0 in 30 min at pH 8.0 at 37 C (reaction volume 2.625 ml). Assay: ...
Company:Sigma-Aldrich
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(Date:4/18/2014)... have made a brain-related discovery that could revolutionize ... This discovery opens up the possibility that environmental ... specific brain activity that the researchers have identified ... Journal of Neuroscience has just published a ... associate professor of psychology and his doctoral student ...
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(Date:4/17/2014)... have identified the first genetic variant specifically associated ... accounting for around 10-15 per cent of all ... of the breast cancer sub-type, called invasive lobular ... causes of this particular kind of breast cancer, ... research, published today (Thursday) in the journal ...
(Date:4/17/2014)... According to a study published today in PLOS ... world where malaria is common can mount an immune ... them to avoid repeated bouts of high fever and ... in their bloodstream. The findings may help researchers develop ... by the malaria parasite. , Each year, approximately 200 ...
(Date:4/17/2014)... Two recent papers by a University of Colorado ... scientists develop treatments or vaccines for Dengue fever, ... other disease-causing flaviviruses. , Jeffrey S. Kieft, PhD, ... the School of Medicine and an early career ... colleagues recently published articles in the scholarly journals ...
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(Date:4/17/2014)... consumption advisories for expecting mothers are ineffective in ... organic pollutants (POPs). , The study, performed ... Toronto Scarborough PhD student Matt Binnington and Professor ... environmental contamination, a mother,s compliance with advisories, and ... exposure in her children. , Their model estimates ...
(Date:4/17/2014)... A Kansas State University engineer has developed ... remotely detects improvised explosive devices. The same technique ... Dunn, the Steven M. and Kay L. Theede ... and nuclear engineering, and his research team have ... underground or in car trunks. The distance detection ...
(Date:4/17/2014)... Europe, Asia and the United States are spreading across ... native honeybee populations at this time, according to an ... Nosema microsporidia and Varroa mites., ... these invasive pests, which suggests to us that the ... the United States currently are not necessary in East ...
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