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siPORT in Biological Technology

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

...on.com/catalog/CatNum.php?4502">4502 siport Amine Transfection Agent ...on.com/catalog/CatNum.php?4503">4503 siport Amine Transfection Agent ...on.com/catalog/CatNum.php?4504">4504 siport Lipid Transfection Agent ...

The let-7 microRNA Family Regulates RAS: Implications for Development and Oncogenesis

... Figure 1. let-7 Influences the Expression of RAS in Human Cells. HepG2 cells were transfected in 24 well plates using siport NeoFX (Ambion) with either 30 nM let-7 a Pre-miR miRNA Precursor Molecule (Ambion) or a negative control miRNA inhibitor ...

Streamline Your siRNA Transfections

... NEW siport NeoFX Transfection Agent Pr... making optimization a relatively slow process. siport NeoFX Transfection Agent was developed to refine siRNA transfection protocols. siport NeoFX lipid-based formulation can be ...

Setting up Successful siRNA Library Screens

... Screening siRNAs targeting hundreds of genes requires conditions for high-throughput transfection. siport NeoFX facilitates the transfection of up to 1,000 wells in less than an hour without the need for robotics ...

RNAi: The Controls You Need

.... Figure 1. Titration of Silencer Validated siRNAs. The indicated siRNAs were complexed with siport Lipid Transfection Reagent (Ambion) and the resulting complexes were added to HeLa cells in 24 well plates at the final concen...

RNAi: Get the Whole Story

...ll into 24 well culture plates. The cells were transfected using siport Lipid with an siRNA targeting p53 (293T) or Stat-1 (HeLa) or w... Control #1 (scrambled) at a final concentration of 100 nM using siport Lipid . Duplicate samples were harvested at 72 hours and each d...

RNAi: A How To for New Users

...odGrp=291">siPORT Lipid and siport Amine Transfection Agents ...on.com/catalog/CatNum.php?4502">4502 siport Amine Transfection Agent ...on.com/catalog/CatNum.php?4504">4504 siport Lipid Transfection Agent ...

Recommendations for Successful siRNA Library Screens

...es in Cells Transfected with Distinct siRNAs. Panel A . HeLa cells (5,000 per well in 96-well plates) were transfected using siport NeoFX (Ambion) in triplicate with three different siRNAs targeting each of 59 different kinase-specific siRNAs. Three days pos...

Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells

... hsa-mir-196 (miR-196), respectively, using siport NeoFX Transfection Agent (Ambion). Re...iR miRNA Inhibitors in triplicate using siport NeoFX Transfection Agent (Ambion). 72 hr post-tr...on.com/catalog/CatNum.php?4502">4502 siport Amine Transfection Agent ...

Optimize Transfection of siRNAs for RNAi

... types of transfection agents (siPORT Amine and siport Lipid ) in either serum free or serum ...ected in serum supplemented media using siport Amine Agent. Figure 1. Ef... or negative control siRNA using either siport Amine or...
siPORT in Biological Products

siPORT NeoFX Transfection Agent from Ambion

Description:...ments often call for critical, but time-consuming optimization experiments. siport NeoFX Transfection Agent refines siRNA transfection protocols resulting in less optimization. siport NeoFXs lipid-based formulation can be used to efficiently transfect adheren...
Company:Ambion

siPORT NeoFX Transfection Agent 1 ml from Ambion

Description:...ments often call for critical, but time-consuming optimization experiments. siport NeoFX Transfection Agent refines siRNA transfection protocols resulting in less optimization. siport NeoFXs lipid-based formulation can be used to efficiently transfect adheren...
Company:Ambion
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