Cancer cells need normal, nonmutated genes to survive

...ent in many deadly cancers, and researchers have not been successful in developing drugs against the dangerous gene. The team used molecules called shrnas to interfere with the production of thousands of normal, healthy proteins in the two cell lines. They gave the cells time to divide and sifted through...

The vulnerable cancer cell

...o use the Broad's automated RNAi screening technology to assess about 5,000 shrnas targeting about 1,000 human genes in a panel of eight human cancer cell lines. The shrnas were carried in lentiviral vectors, which the researchers used to infect fo...

UCSF creates fast, affordable tool for finding gene 'on-off' switches

...is known as RNA interference. Until now, however, the process of finding shrnas that are able to potently deactivate a given gene has been extremely labori...ystem, which uses "deep sequencing" technology to rapidly uncover effective shrnas by simultaneously sequencing millions of base pairs of DNA, also enables sc...

CSHL scientists correlate enzyme expression levels with chemotherapy drug response

...In these experiments we chose to survey a group of shrnas that target a set of genes that scientists call 't...rch has identified." In all, there are about 2,300 shrnas in this cancer-specific set that target genes in t... or "pools" of them, the team was able to identify shrnas that specifically interfered with the expression o...

CSHL scientists trace causal link between a tumor suppressor gene and liver cancer

...ouse liver cells in which the gene was not expressed. Short-hairpin RNAs (shRNAs) were designed to "knock down" DLC1 expression in vivo. When the DLC1 shrnas are introduced into normal mouse liver cells, the production of DLC1 protein is blocked, or 'silenced.' The team observed that nearly all of the mice ...

shRNAs in Biological Technology

A Screen of shRNAs Targeting Tumor Suppressor Genes to Identify Factors Involved in Paclitaxel Sensitivity

Diana Ji, Stacy L. Deeds, and Edward J. Weinstein RNA interference methodologies have been utilized in small to large scale screening projects. The technology has allowed researchers to perform gene-silencing experiments in timeframes and target cells prev...

RNA Interference and Gene Silencing: History and Overview

... structure with 3' UU-overhangs. The ends of the shrnas are processed in vivo, converting the shrnas into ~21 nt siRNA-like molecules, which in ... The siRNA strands from this vector, like the shrnas of the other vectors, have 5 thymidine te...

Uses and Applications of FuGENE 6 Transfection Reagent

...ication of FuGENE 6 Transfection Reagent in Vector-based shRNA Experiments RNA interference induced by siRNAs or shrnas allows reliable down-regulation of the expression of specific genes in human and other mammalian cells. While siRNAs introduced into cells are u...