Scientists develop method for comprehensive proteome analysis
...tein complement (proteome) in Schizosaccharomyces pombe
( S. pombe
) fission yeast.
Laurence Brill, Khatereh Motam...e. The remaining 400 are only expressed during S. pombe
's mating state.
The structure of the Mre11 protein bound to DNA
...trand is held apart and prepared for continuing replication after repair.
Now combining sequencing and biochemical studies of Schizosaccharomyces pombe
(fission yeast), by Paul Russell and his lab at Scripps, with models based on images of the P. furiosus Mre11-DNA complexes, the researchers learne...
Study suggests that publicly available genome data may contain small but significant errors
...d snippets from the genome of the fission yeast S. pombe
and examined how they bind to probes arrayed on a...g the genome. The resulting physical map of the S. pombe
genome was compared to the corresponding map computed from publicly available S. pombe
sequence. The comparison showed a small number of ...
pombe in Biological Technology
A New C-Terminal GST Vector for Protein Production in S. pombe
Versatile eukaryotic expression and protein purification vector
Quinn Lu Tanya Hosfield
Stratagene has constructed and tested the new pESP-3 vector, which can be
used for C-terminal fusion with glutathione-S-transferase (GST)
and for protein production i...
Fermentation Cell Culture: University Style
...evisiae in our laboratory, but we also grow
and Escherichia coli.
BioPharm: For what purpose... E. coli ), or multi-protein
complexes ( S. cerevisiae ). For S. pombe
perform exploratory analyses of macromolecular
Transformation of Filamentous Fungi by High-Voltage Electroporation
...1989; Hashimoto et al.,
1985; Hill, 1989; Karube et al., 1985; Meilhoc et al., 1990; Rech et al.,
1990; Weaver et al., 1988), S. pombe
(Hood and Stachow, 1990; Weaver et al., 1988), and D. discoideum (Dynes and Firtel, 1989; Egelhoff et al.,
1989; Howard et al., 1988). This...
Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping
.... Under these
conditions, the chromosomes of Schizosaccharomyces pombe
lanes 4 and 6), Saccharomyces cerevisiae (open ...ration profiles shown in Figure 2 were derived by running
the S. pombe
and S. cerevisiae chromosomes on 0.5%, 0.4%, 0.3% and 0.2%
Yeast Protein Production System Features High Yields and One-Step
...proteins. By using the yeast
as the host and glutathione-S-transferase
(GST...been created to meet this demand.
The yeast S. pombe
is a unicellular eukaryotic organism, with propert...i
replication origins, competent cells of the S. pombe
host strain and reagents
for protein induction a...
Production of Full-Length, Biologically Active MEK1WT and MEK1CA
...s GST fusion proteins
in Schizosaccharomyces pombe
using the ESP expression and
purification syst...xpression of GST-MEK1 WT and GST-MEK1 CA in
Stratagenes new ESP yeast protein expression a...pression constructs were transformed
into the S. pombe
host strain SP-Q01 (leu1-32h - ). The
New Yeast Cloning System for Producing Proteins with Native Amino Acid
and high-yield production of recombinant proteins in a eukaryotic organism.
Because the system uses the yeast Schizosaccharomyces pombe
the expression host, proteins undergo many of the posttranslational modifications
that are often required for their biologica...
Simple, Sensitive, and Rapid Detection of FLAG -Tagged
fusion protein purified from Schizosaccharomyces pombe
as the test
sample. As shown in Figure
3 , as low as... ng/ml of the fusion protein can be detected. We further
used S. pombe
lysates to detect a GST-FLAG-MEK1 fusion protein
pombe in Biological Products
pombe in Biological Definition
...ly of the kinetochore on the centromere and may play a role in the epigenetic inheritance of the centromere site.
In the yeast Schizosaccharomyces pombe
(and likely in other eukaryotes) the formation of centromeric heterochromatin is connected to RNAi * . In nematodes such as Caenorhabditis elegan...
...omprehensively studied and understood fungi are the yeasts and eukaryotic model organisms Saccharomyces cerevisiae and Schizosaccharomyces pombe
Many fungi produce toxins , antibiotics , and other secondary metabolites . For example, the cosmopolitian (worldwide) genus Fusarium and ...
pombe in Biological Dictionary
...n of S phase from the Merriam-Webster Online Dictionary with audio pronunciations, thesaurus, Word of the Day, and word games. The Forsburg Lab pombe
pages: fission yeast DNA replication ... This page describes S phase control in fission yeast, and provides links to ... Damage to DNA afte...
...ing, cyclin-CDK complexes. ... G1 is a crucial phase of cell growth because the decision to begin another ... response of Schizosaccharomyces pombe
cells to UV irradiation in early G1 phase . ... a decision in the G1 phase of the division cycle. either to proceed through the cell c...
...eporter Vector System ... This validated reporter system contains two mammalian expression vectors (see figure at right) ... The Forsburg lab pombe
pages: directory of fission yeast plasmids ... full strength nmt expression vector with 12x myc tag fusion at C-terminus. ... 37000 Full Length...
...the Science Citation Index ... Offers information on the cell cycle , including prokaryotic and eukaryotic cell division. Background on the pombe
cell cycle can be found on our site. Problems printing? ... Question of cell cycle regulation: how to ensure the orderly progression of ... ...