Scientists develop method for comprehensive proteome analysis

...tein complement (proteome) in Schizosaccharomyces pombe ( S. pombe ) fission yeast. Laurence Brill, Khatereh Motam...e. The remaining 400 are only expressed during S. pombe 's mating state. S....

The structure of the Mre11 protein bound to DNA

...trand is held apart and prepared for continuing replication after repair. Now combining sequencing and biochemical studies of Schizosaccharomyces pombe (fission yeast), by Paul Russell and his lab at Scripps, with models based on images of the P. furiosus Mre11-DNA complexes, the researchers learne...

Study suggests that publicly available genome data may contain small but significant errors

...d snippets from the genome of the fission yeast S. pombe and examined how they bind to probes arrayed on a...g the genome. The resulting physical map of the S. pombe genome was compared to the corresponding map computed from publicly available S. pombe sequence. The comparison showed a small number of ...

pombe in Biological Technology

A New C-Terminal GST Vector for Protein Production in S. pombe

Versatile eukaryotic expression and protein purification vector Quinn Lu Tanya Hosfield Stratagene Stratagene has constructed and tested the new pESP-3 vector, which can be used for C-terminal fusion with glutathione-S-transferase (GST) and for protein production i...

Fermentation Cell Culture: University Style

...evisiae in our laboratory, but we also grow Schizosaccharomyces pombe and Escherichia coli. BioPharm: For what purpose... E. coli ), or multi-protein complexes ( S. cerevisiae ). For S. pombe we perform exploratory analyses of macromolecular comp...

Transformation of Filamentous Fungi by High-Voltage Electroporation

...1989; Hashimoto et al., 1985; Hill, 1989; Karube et al., 1985; Meilhoc et al., 1990; Rech et al., 1990; Weaver et al., 1988), S. pombe (Hood and Stachow, 1990; Weaver et al., 1988), and D. discoideum (Dynes and Firtel, 1989; Egelhoff et al., 1989; Howard et al., 1988). This...

Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping

.... Under these conditions, the chromosomes of Schizosaccharomyces pombe (closed circles, lanes 4 and 6), Saccharomyces cerevisiae (open ...ration profiles shown in Figure 2 were derived by running the S. pombe and S. cerevisiae chromosomes on 0.5%, 0.4%, 0.3% and 0.2% Chrom...

Yeast Protein Production System Features High Yields and One-Step Purification

...proteins. By using the yeast Schizosaccharomyces pombe as the host and glutathione-S-transferase (GST...been created to meet this demand. The yeast S. pombe is a unicellular eukaryotic organism, with propert...i replication origins, competent cells of the S. pombe host strain and reagents for protein induction a...

Production of Full-Length, Biologically Active MEK1WT and MEK1CA

...s GST fusion proteins in Schizosaccharomyces pombe using the ESP expression and purification syst...xpression of GST-MEK1 WT and GST-MEK1 CA in S. pombe Stratagenes new ESP yeast protein expression a...pression constructs were transformed into the S. pombe host strain SP-Q01 (leu1-32h - ). The cells were...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

... and high-yield production of recombinant proteins in a eukaryotic organism. Because the system uses the yeast Schizosaccharomyces pombe as the expression host, proteins undergo many of the posttranslational modifications that are often required for their biologica...

Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins

...FLAG-calmodulin fusion protein purified from Schizosaccharomyces pombe as the test sample. As shown in Figure 3 , as low as... ng/ml of the fusion protein can be detected. We further used S. pombe lysates to detect a GST-FLAG-MEK1 fusion protein expressed under...

pombe in Biological Products

Rabbit Anti-Schizosaccharomyces pombe SpUlp2 Polyclonal Antibody, Unconjugated from Abcam

Description: Rabbit polyclonal to SpUlp2 ( Abpromise for all tested applications). Antigen: Synthetic peptide: SNNERQSLSSGSND, corresponding to amino acids 622-635 of Schizosaccharomyces pombe SpUlp2...
Company:Abcam

S. pombe Whole Genome ChIP-on-chip from Agilent Technologies

Description: The S. pombe Whole Genome ChIP-on-chip Microarray is specifically designed for location analysis of yeast (S. pombe) DNA binding proteins by pairing chromatin immunoprecipitation (ChIP) with Agilent DNA microarrays. This set delivers robust hybridization and reliable binding data with greater true ...
Company:Agilent Technologies

Casein Kinase 1 Active from Upstate

Description:...tography Quality Assurance: routinely evaluated by phosphorylation of Casein Kinase 1 Substrate Peptide (Catalog # 12-423) pombe gene cki1 protein expressed in E. coli...
Company:Upstate

Mouse Anti-Nop1p / Fibrillarin Monoclonal Antibody, Unconjugated, Clone A00104.01 from GenScript Corporation

Description:...eins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fi...
Company:GenScript Corporation

pombe in Biological Definition

Centromere

...ly of the kinetochore on the centromere and may play a role in the epigenetic inheritance of the centromere site. In the yeast Schizosaccharomyces pombe (and likely in other eukaryotes) the formation of centromeric heterochromatin is connected to RNAi * . In nematodes such as Caenorhabditis elegan...

Mycology

...omprehensively studied and understood fungi are the yeasts and eukaryotic model organisms Saccharomyces cerevisiae and Schizosaccharomyces pombe . Many fungi produce toxins , antibiotics , and other secondary metabolites . For example, the cosmopolitian (worldwide) genus Fusarium and ...

pombe in Biological Dictionary

S phase

...n of S phase from the Merriam-Webster Online Dictionary with audio pronunciations, thesaurus, Word of the Day, and word games. The Forsburg Lab pombe pages: fission yeast DNA replication ... This page describes S phase control in fission yeast, and provides links to ... Damage to DNA afte...

G1 phase

...ing, cyclin-CDK complexes. ... G1 is a crucial phase of cell growth because the decision to begin another ... response of Schizosaccharomyces pombe cells to UV irradiation in early G1 phase . ... a decision in the G1 phase of the division cycle. either to proceed through the cell c...

Expression Vector

...eporter Vector System ... This validated reporter system contains two mammalian expression vectors (see figure at right) ... The Forsburg lab pombe pages: directory of fission yeast plasmids ... full strength nmt expression vector with 12x myc tag fusion at C-terminus. ... 37000 Full Length...

Cell cycle

...the Science Citation Index ... Offers information on the cell cycle , including prokaryotic and eukaryotic cell division. Background on the pombe cell cycle can be found on our site. Problems printing? ... Question of cell cycle regulation: how to ensure the orderly progression of ... ...