Pichia

Biologists at UC San Diego identify key protein in cell's 'self-eating' function

...esearchers report in their paper that they identified a novel protein called Atg30 (one of 31 required for autophagy-related processes) from the yeast pichia pastoris, that controls the degradation of a sub-compartment of cells, the peroxisomes. Peroxisomes generate and dispose of harmful peroxides that ...

Upgraded IMG 2.3 Data Management System released by DOE JGI to eager user community

... (eukaryotic unicellular organisms), and plant genomes to enhance the breadth of comparative analysis. A new addition of particular interest to DOE is pichia stipitis CBS 6054, a fungus with the exceptional capability to ferment xylose, five-carbon wood sugar, and yield high levels of ethanol. "Using com...

All eukaryotic kinases share 1 common set of substrates

...hey incubated them with radio actively labelled ATP as a source of phosphate, and lysates (cell content) of two species of yeast (Candida albicans and pichia pastoris), a fungus (Fusarium solani), a plant (Triticum aeastivum, wheat), a fruit fly (Drosophila melanogaster), a mouse (Mus musculus), and man (Ho...

Super-fermenting fungus genome sequenced

...cterization of the genetic blueprint of the fungus pichia stipitis, by the U.S. Department of Energy Joint G...The information embedded in the genome sequence of pichia has helped us identify several gene targets to improve xylose metabolism,?said pichia paper lead author Thomas W. Jeffries of the Forest...

GlycoFi and Dartmouth report full humanization of yeast glycosylation pathway in Science

...k & Co., Inc., and their collaborators at Dartmouth-Hitchcock Medical Center published their report detailing the genetic engineering of the yeast pichia pastoris to secrete human glycoproteins with fully complex, terminally sialyated N-glycans today in the September 8, 2006 issue of the journal Scienc...

Pichia in Biological Technology

140-Fold Increase of Protein Achieved in Pichia Fermentation

NBS microbiologists recently assisted researchers at the University of California in achieving a 140-fold increase in protein production from a recombinant strain of Pichia pastoris yeast. Top yields by the researchers thus far had been 1.4 mg/L of a throm-bomod...

High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation

By Julia Cino, PhD Introduction Over the last several decades, geneticists have learned how to manipulate DNA to identify, excise, move and place genes into a variety of organisms that are quite different genetically from the source organism. A ma...

Mylan Announces Strategic Collaboration with Biocon to Enter the Global Generic Biologics Market

...d solutions to partners and customers across the globe. Biocon launched the world's first recombinant human insulin, INSUGEN(R) in November 2004 using pichia expression and India's first indigenously produced monoclonal antibody BIOMAb-EGFR(TM). Focusing on unmet medical needs in cancer, diabetes and inflam...

DOE JGI Director Eddy Rubin highlights the genomics of plant-based biofuels in the journal Nature

...rade lignin by gaining access through the protective matrix surrounding the cellulose microfibrils of plant cell walls. Another fungus, the yeast pichia stipitis, ferments the five-carbon "wood sugar" xylose abundant in hardwoods and agricultural harvest residue. Rubin says that Pichia's recently seque...

P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor

...ant cells and insect cells. pichia pastoris: pichia pastoris is a methlotrophic yeast, which provides...egulatory proteins at various different levels. pichia pastoris is also the only system that offers th...

Proteinase K, Recombinant, PCR Grade: the Ideal Tool for Template Preparations

... The endopeptidase originally isolated from the filtrate of the lower fungus Tritirachium album limber is recombinantly expressed in pichia pastoris. Proteinase K is extremely effective on native proteins from different sources, and remains active in the presence of denaturing reagen...

Pichia in Biological Products

SMD1168 from Invitrogen

Description:... Several pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest. Table 1 provides information to help you ...
Company:Invitrogen

Spheroplast Kit for Yeast from Invitrogen

Description:... The Spheroplast Kit contains qualified reagents for the preparation of yeast spheroplasts. The reagents have been optimized for use with pichia pastoris and are guaranteed to generate 70% spheroplasts....
Company:Invitrogen

Pichia Protocols: Methods in Molecular Biology from Invitrogen

Description:... pichia Protocols is the first all-inclusive collection of key procedures for use of the pichia pastoris Expression System. Methods developed by the leaders in pichia expression are provided as detailed, step-by-step ...
Company:Invitrogen

pPIC9K from Invitrogen

Description:...ticin Reagent in Pichia. Spontaneous generation of multiple insertion events can be identified by resistance to increased levels of Geneticin Reagent. pichia transformants are selected on histidine-deficient medium and screened for their level of resistance to Geneticin Reagent. The ability to grow in high ...
Company:Invitrogen

Fermentation System fedbatch pro® from DASGIP AG

Description:...t 4 up to 16 culture vessels High precision with small-scale requirements provides users with proven fermentation results when working with E.coli, pichia pastoris, yeast, fungi or any other microbial application. fedbatch pro® features the ability to independently control 4, 8 or 16 vessels with ...
Company:DASGIP AG

Enterokinase EKMax from Invitrogen

Description:...; is a recombinant preparation of the catalytic subunit of bovine enterokinase (1). EKMax™ Enterokinase is expressed and isolated from the yeast pichia pastoris to yield a highly purified enzyme preparation (2). It recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysi...
Company:Invitrogen

Yeast Nitrogen Base from Invitrogen

Description:...oes not contain amino acids. Both bulk and convenient single-use pouches are available. Each pouch contains reagents to prepare 500 ml of 10X YNB for pichia media or 1000 ml of 10X YNB for S. cerevisiae media. Store at 15C to 30C. Guaranteed stable for 6 months when properly stored....
Company:Invitrogen

Yeast Nitrogen Base from Invitrogen

Description:...oes not contain amino acids. Both bulk and convenient single-use pouches are available. Each pouch contains reagents to prepare 500 ml of 10X YNB for pichia media or 1000 ml of 10X YNB for S. cerevisiae media. Store at 15C to 30C. Guaranteed stable for 6 months when properly stored....
Company:Invitrogen

Pichia in Biological Dictionary

Host strain

...uct ... SCS-8 E. coli Strain ( Host ) Agilent Technologies - Stratagene Products. 1 mL glycerol stock ... Host Strains (Yeast) Brewers Yeast. pichia pastoris Strains ... Strains From ZymoResearch. XJa and XJb, the two conditionally autolytic E. coli strains ... The Morningstar Strain mos...