New tool isolates RNA within specific cells
...UO project as a postdoctoral fellowship funded by the National Institutes of Health and HHMI.
Cleary's group built its tool with the enzyme uracil
phosphoribosyltransferase (UPRT), a nucleotide salvage enzyme that prepares nucleotides for incorporation into newly synthesized RNA. By altering the nucleotide analog 4-thiour...
phosphoribosyltransferase in Biological Products
phosphoribosyltransferase in Biological Definition
Monoclonal antibody
...ntibodies, so as to not dilute the target antibody. Furthermore, one uses only myeloma cells that have lost a specific enzyme ( hypoxanthine-guanine
phosphoribosyltransferase , HGPRT) and therefore cannot grow under certain conditions (namely in the presence of HAT medium ). Fusions between healthy B-cells and myeloma cells...
Purine
...ood) can also be salvaged and reused in new nucleotides. The enzyme adenine
phosphoribosyltransferase salvages adenine, while hypoxanthine-guanine
phosphoribosyltransferase (HPRT) salvages guanine and hypoxanthine. Genetic deficiency of HPRT cause...
phosphoribosyltransferase in Biological Dictionary
Guanine
...eaminase · AMP deaminase · Inosine monophosphate ... Hypoxanthine- guanine
phosphoribosyltransferase - Adenine
phosphoribosyltransferase ... Guanine - Definition of Guanine at Dictionary.com a free online ...