Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer

Improve PCR product yield and specificity for difficult amplifications Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer Frances Bai Holly Hogrefe Stratagene Certain PCR amplification systems produce multiple product bands or ...

Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase

High-fidelity PCR of genomic targets up to 19 kb Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase Michael Borns Holly Hogrefe Stratagene Improve amplification of long targets with PfuTurbo DNA polymerase* by making simple modifications to PCR reacti...

PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases

PCR Performance Comparisons Between PfuTurbo and Taq DNA Polymerases pfuturbo DNA polymerase is the superior choice for robust high-fidelity PCR amplification Holly Hogrefe Frances Bai Janice Cline Stratagene In this update, we compare the PCR performan...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

...erformed with a Bio-Rad Gene Cycler using PfuTM or pfuturbo polymerase. Two different primer pairs,...l containing 2.5 units of cloned Pfu or pfuturbo (DNA polymerase Strategene, San Diego, CA), ...mize non-specific annealing of the primers, Pfu or pfuturbo polymerase was added after the first de...

The TripleMaster PCR System for three different amplifications

...acy that is normally only achieved using products that are specifically designed for high-fidelity applications. Although the tested pfuturbo System (Stratagene) is revealed to have the lowest error rate, it is suitable only for the amplification of very short sequences (< ...

Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance

...ading DNA polymerases except Stratagenes Pfu and pfuturbo DNA polymerases. * , Herculase enhance...ercial proofreading DNA polymerases except Pfu and pfuturbo DNA polymerases ( Figure 7 )...r than that of Herculase DNA polymerase. While pfuturbo remains the best choice for routine PCR applicatio...

Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications

... Enhanced DNA Polymerase pfuturbo DNA Polymerase ... ( Std. dev.) pfuturbo 4 ...found to be 2.1-fold higher than the error rate of pfuturbo DNA polymerase and 1.6-fold and 3.7-fol...

Performance Comparisons of Commercial RT-PCR Systems

... The ProSTAR ultra HF RT-PCR system, featuring pfuturbo DNA polymerase** and a versatile two-step form... versatile two-step format is used, which features pfuturbo DNA polymerase, 3 a special formulation of Pfu ... HF RT-PCR system is related to the use pfuturbo DNA polymerase for cDNA amplification. Additional ...

pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning

...s that are easily cloned with high efficiency pfuturbo DNA Polymerase: A High-Performance, High-Fideli...loramphenicol resistance (CAM) gene generated from pfuturbo DNA polymerase * , was cloned into the PCR-Sc...equent cloning. Stratagene recently introduced pfuturbo DNA polymerase, 1 a special formulation of clon...

prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

.... The ProSTAR Ultra HF RT-PCR system features pfuturbo DNA polymerase * for amplifying cDNAs with the...ons (10 6 -fold amplification) using Stratagenes pfuturbo DNA polymerase (error rate of 1.3 x 10 -6 M.F./...nal clones would contain mutations introduced by pfuturbo DNA polymerase [(1.3 x 10 -6 )(1000 bp)(20 dupli...