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pSilencer in Biological Technology

Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function

Iezzi M, Kouri G, Fukuda M, Wollheim CB (2004) Synaptotagmin V and IX isoforms control Ca 2+ -dependent insulin exocytosis. J Cell Sci 117:3119-3127. In a recently published paper in the ...

Selecting siRNA Sequences to Incorporate into the pSilencer Vectors

The first step in preparing a plasmid for siRNA experiments is to identify target sequences in the gene of interest that are susceptible to siRNA-induced degradation. We have found that a little more than half of the siRNAs provide at le...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

... Figure 1. siRNA-mediated Knockdown of GAPDH Using psilencer 4.1-CMV puro. HeLa cells were not transfected (NT) or transfect...ected. RU = relative units. The psilencer 4.1-CMV vectors are provided linearized and ready for...

siRNA Design Guidelines

...e III. For traditional cloning into psilencer vectors, two DNA oligonucleotides t...e. In addition, for cloning into the psilencer 1.0-U6 vector, nucleotide overhangs ... 2). In contrast, for cloning into the psilencer 2.0-U6, 2.1-U6, 3.0-H1, or 3.1-H1 v...
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