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pSilencer in Biological Technology

Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function

Iezzi M, Kouri G, Fukuda M, Wollheim CB (2004) Synaptotagmin V and IX isoforms control Ca 2+ -dependent insulin exocytosis. J Cell Sci 117:3119-3127. In a recently published paper in the ...

Selecting siRNA Sequences to Incorporate into the pSilencer Vectors

The first step in preparing a plasmid for siRNA experiments is to identify target sequences in the gene of interest that are susceptible to siRNA-induced degradation. We have found that a little more than half of the siRNAs provide at le...

pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

... Figure 1. siRNA-mediated Knockdown of GAPDH Using psilencer 4.1-CMV puro. HeLa cells were not transfected (NT) or transfect...ected. RU = relative units. The psilencer 4.1-CMV vectors are provided linearized and ready for...

siRNA Design Guidelines

...e III. For traditional cloning into psilencer vectors, two DNA oligonucleotides t...e. In addition, for cloning into the psilencer 1.0-U6 vector, nucleotide overhangs ... 2). In contrast, for cloning into the psilencer 2.0-U6, 2.1-U6, 3.0-H1, or 3.1-H1 v...
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Breaking Medicine News(10 mins):Health News:Biostimulants Market in Turf and Ornamentals is Expected to Reach $ 3.8 Billion by 2018 - New Report by MicroMarket Monitor 2Health News:Biostimulants Market in Turf and Ornamentals is Expected to Reach $ 3.8 Billion by 2018 - New Report by MicroMarket Monitor 3Health News:Dengue Fever Vaccine Shows Some Promise in Trial 2Health News:Dengue Fever Vaccine Shows Some Promise in Trial 3Health News:Riverside and Williamsburg Landing Partner to Offer First of Its Kind Service to Virginians Who Want to Age in Place 2Health News:Riverside and Williamsburg Landing Partner to Offer First of Its Kind Service to Virginians Who Want to Age in Place 3Health News:Riverside and Williamsburg Landing Partner to Offer First of Its Kind Service to Virginians Who Want to Age in Place 4Health News:Americas Pigments Market Worth 1,099 Thousand MT by 2018 - New Report by MicroMarket Monitor 2Health News:Americas Pigments Market Worth 1,099 Thousand MT by 2018 - New Report by MicroMarket Monitor 3
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Breaking Biology News(10 mins):Molecular snapshots of oxygen formation in photosynthesis 2
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