Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function
Iezzi M, Kouri G, Fukuda M, Wollheim CB
(2004) Synaptotagmin V and IX isoforms control
Ca 2+ -dependent insulin exocytosis. J Cell
In a recently published paper in the
Selecting siRNA Sequences to Incorporate into the pSilencer Vectors
The first step in preparing a plasmid for siRNA
experiments is to identify target sequences in the gene of interest
that are susceptible to siRNA-induced degradation. We have found
that a little more than half of the siRNAs provide at le...
pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
Figure 1. siRNA-mediated Knockdown of GAPDH Using
4.1-CMV puro. HeLa cells were not transfected (NT)
RU = relative units.
are provided linearized and ready for...
siRNA Design Guidelines
For traditional cloning into psilencer
vectors, two DNA
oligonucleotides t...e. In addition, for
cloning into the psilencer
1.0-U6 vector, nucleotide overhangs
2). In contrast, for cloning into the psilencer
2.1-U6, 3.0-H1, or 3.1-H1 v...