Direct transfer of starter substrates from type I fatty acid synthase ...
The vector plasmids pETDuet, pACYC, pCDF, and pRSF, which contain different ... E. coli harboring pETDuet–ArsAD, pACYC–ArsB, and pRSF–ACC produced three ...P54nrb Forms a Heterodimer with PSP1 That Localizes to Paraspeckles in ...
PETDuet-1-6HisPSP1/p54nrb was made by PCR of PSP1 and p54nrb as ... The resultant product was then cloned into pETDUET-1 MCS2 cut with the same enzymes. ...Vectors for co-expression of an unrestricted number of proteins ...
The pETDuet system of Novagen allows construction of a co-expression plasmid for ... the expression achieved by our system to the pETDuet co-expression system. ...Lyase Activities of CpcS- and CpcT-like Proteins from Nostoc PCC7120 ...
When pETDuet-derived plasmids were used, the gene in pET30 was transferred in ... pho1-pcyA, pETDuet-cpcT1, and pCDF-cpcS1 were co-transformed into E. coli BL21 ...The KCNQ1 (Kv7.1) COOH Terminus, a Multitiered Scaffold for Subunit ...
This pETDuet vector contains a His8 tag sequence and TEV protease recognition ... then served as the template for PCR of an insert for the pETDuet vector. ...