pCMV

Aurum Plasmid 96 Kit Tech Note, Rev A

...gation E. coli strain DH5α (Invitrogen) containing plasmid pcmv (Stratagene) was streaked out on an LB-ampicillin (100 g/ml) aga...locks blotted on paper towels to remove residual medium. Plasmid pcmv was subsequently purified from the bacteria using a different ma...

Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment

...actosidase gene activity under in vitro and in vivo conditions. The plasmid pcmv without the -galactosidase gene was applied as a negative contro...MV--gal-coated 1.0 m gold microcarriers. Mice bombarded with the pcmv plasmid represented the negative control. One week after inocula...

High-Efficiency Transfections Achieved with New Low-Toxicity Reagent

... optimized transfection conditions for GeneJammer and each of the five most common transfection reagents (products a, b, c, d, and e). We used the pcmv b-gal reporter plasmid (0.4 g/well) to transfect CHO-K1, COS-7, 293, HeLa, human umbilical arterial endothelial cells (HUAEC), and human umbilical...

A Simple and Rapid Technique to Purify High-Quality Midiprep DNA

... plasmid DNA was also recovered from a 100-ml overnight bacterial culture using a single midispin cup, while the low-copy number pcmv gal plasmid yield from a 200-ml overnight culture was 250 g ( Figure 3 ). Yields and spectrophotometric values (A 260 /A 280 ...

pCMV in Biological Dictionary

CDNA clone

... FL cDNA clones : Searchable collection of over 33,000 non-redundant, non-PCR-generated human full-length cDNA clones in pcmv based expression vectors A searchable gene bank of over 33,000 human full-length cDNA clones suitable and available for high-throughput ...