Aurum Plasmid 96 Kit Tech Note, Rev A
E. coli strain DH5α (Invitrogen) containing plasmid pcmv
was streaked out on an LB-ampicillin (100 g/ml) aga...locks blotted
on paper towels to remove residual medium. Plasmid pcmv
purified from the bacteria using a different ma...
Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
...actosidase gene activity under in vitro and in vivo conditions. The plasmid pcmv
without the -galactosidase
gene was applied as a negative contro...MV--gal-coated 1.0 m gold microcarriers. Mice
bombarded with the pcmv
plasmid represented the negative control. One
week after inocula...
High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
... optimized transfection conditions for
GeneJammer and each of the five most common transfection reagents (products a,
b, c, d, and e). We used the pcmv
plasmid (0.4 g/well) to transfect CHO-K1, COS-7, 293, HeLa, human umbilical
arterial endothelial cells (HUAEC), and human umbilical...
A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
... plasmid DNA was also recovered from a 100-ml
overnight bacterial culture using a single midispin cup, while the low-copy
gal plasmid yield from a 200-ml overnight culture was 250
g ( Figure
3 ). Yields and spectrophotometric values (A 260 /A 280 ...
... FL cDNA clones : Searchable collection of over 33,000 non-redundant, non-PCR-generated human full-length cDNA clones in pcmv
based expression vectors A searchable gene bank of over 33,000 human full-length cDNA clones suitable and available for high-throughput ...