Using Multiphoton Microscopy to See Chromosomes in Action

Feverish fruit fly larvae, warmed in a toasty lab chamber, are giving Cornell researchers a way to watch chromosomes in action and actually see how genes are expressed in living tissue.// Using multiphoton fluorescence microscopy, a technique pioneered at Cornell by physicist Watt W. Webb, res...

Researchers discover new battleground for viruses and immune cells

...n infected the animals with vaccinia virus, the virus used to make smallpox vaccine, engineered to express a brilliantly colored protein. Using a multiphoton microscope, a highly specialized microscope that enables scientists to peer into a living organism, the scientists could now look into the lymph nodes...

World class technology and talent battle cancer at the Centenary Institute

...and microscopy, the unique imaging features of the multiphoton microscope will enable scientists at the Centenary...e to develop improved immuno-therapies. Using the multiphoton microscope, Professor Weningers team pioneered gro...as said, The arrival of Professor Weninger and the multiphoton microscope marks a new era in medical research for...

Epilepsy-induced brain cell damage prevented in the laboratory

... Led by postdoctoral fellows Ling-hui Zeng, M.D., Ph.D. and Lin Xu, Ph.D., a team of researcher's in Wong's laboratory applied an approach known as multiphoton imaging to track brain cell changes during seizures. They used a drug to induce seizures in mice and imaged brain cells before, during and after seizu...

multiphoton in Biological News

Researchers use multiphoton microscopy to watch chromosomes in action

Feverish fruit fly larvae, warmed in a toasty lab chamber, are giving Cornell researchers a way to watch chromosomes in action and actually see how genes are expressed in living tissue. Using multiphoton fluorescence microscopy, a technique pioneered at Cornell by physicist Watt W. Webb, research...

Looking at neurons from all sides

... in three dimensions, giving them a much better view of its activity. A multiphoton microscope looks much like a conventional, upright microscope but it has an adaption that allows it to look at tissues in sections. A conventional multiphoton microscope does that very slowly, he said. With ours, you can do it ver...

Research sheds light on the mechanics of gene transcription

...lied physics and the S.B. Eckert Professor in Engineering. Jie Yao, the paper's lead author, recently finished his Ph.D. at Cornell under Webb. Using multiphoton microscopy, a technique developed by Webb that allows high-precision 3D imaging in living cells, the researchers observed polytene chromosomes -- gian...

World's smallest cancer detection device

...tasis and migration ? knowledge that is of critical importance in the treatment and, ultimately, prevention of cancer." Dr. Condeelis has used the multiphoton confocal microscope to directly observe cellular interactions in the tumor microenvironment of live animal models of breast cancer. By placing an arti...

Neuroscientists discover new cell type that may help brain maintain memories of smells

...k advantage of a new type of imaging method called multiphoton microscopy to overcome these hurdles and to discov...that Blanes cells talk to granule cells. "The multiphoton microscope allowed me to identify the axon and the...to just a matter of weeks", said Pressler. The multiphoton microscope used in this study was built by Strowbr...

Findings have implications for tracking disease, drugs at the molecular level

...emission of visible light using a technique called multiphoton absorption induced luminescence (MAIL). The most...ser intensities lower than those commonly used for multiphoton imaging, in which specific tissues or cells -- can...urkas. "The fluorescent molecules commonly used in multiphoton imaging give out only a limited amount of light, '...

The transparent organism: EMBLEM and Carl Zeiss give labs a unique look at life

...ighly resolved image information from living organisms. The products based on this technology will form a perfect match with our lines of confocal and multiphoton 3D-imaging systems," says Dr. Simon. The agreement between EMBL and Carl Zeiss includes a common cooperation project for method optimization and pr...

multiphoton in Biological Technology

A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology

... and nonlinear microscopy including two photon and multiphoton fluorescence microscopy, 9-15 and second and thir... 9. W. Denk and K. Svoboda, "Photon upmanship: why multiphoton imaging is more than a gimmick," Neuron 18, pp. ... R. M. Williams, and W. W. Webb, "Nonlinear magic: multiphoton microscopy in the bio- sciences," Nature Biotechn...