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microdissected in Medical News

Genomic Health's Oncotype DX(R) Colon Cancer Test Predicts Individualized Recurrence Risk for Stage II Colon Cancer Patients

... samples for this analysis of the Onco type DX colon cancer test are from the United Kingdom. Gene expression was quantified by RT-PCR from manually microdissected fixed paraffin-embedded primary colon cancer tissue, and recurrence-free interval, disease-free survival and overall survival were analyzed using Cox ...

MDS Analytical Technologies' Laser Capture Microdissection Helps Facilitate Discovery of Potential New Colon-Cancer Biomarker

...mparing the genetic sequences of the laser capture microdissected colon carcinoma cells with sequences from a genera...igned to maintain biomolecule integrity within the microdissected samples for use in downstream analyses. Whether re...ng IR capture technology allows the custody of the microdissected sample to be maintained throughout the process. Th...

Researchers identify new cell targets for preventing growth of breast and other tumors

...eloped that block these proteins and put a stop to tumor blood vessel growth. In a technique that took three years to perfect, the UNC researchers microdissected individual vascular cells from frozen sections of five cancerous and five normal breast tissue samples. They scanned the activity of thousands of gene...

Racial and ethnic differences in the biology of breast cancer tumors

...and more likely to spread to lymph nodes. Martin and her colleagues examined gene expression profiles an indicator of which genes are active -- in microdissected breast tumors from 35 patients with invasive breast cancer, including 18 African Americans and 17 Caucasians. The researchers identified racial d...
microdissected in Biological News

AIDS-related cognitive impairment exists in two separate forms

... were changed in expression in colon cancer. Boman, who also directs the G.I. Cancer Program at Jefferson's Kimmel Cancer Center, next purified and microdissected colonic crypts, and compared miRNA expression between the bottom tenth of the crypt, where the stem cells reside, and the upper 9/10s of the crypt. Th...

Markers of gene, protein, or micro-RNA activity predict outcome in prostate and colorectal cancers

... were changed in expression in colon cancer. Boman, who also directs the G.I. Cancer Program at Jefferson's Kimmel Cancer Center, next purified and microdissected colonic crypts, and compared miRNA expression between the bottom tenth of the crypt, where the stem cells reside, and the upper 9/10s of the crypt. Th...
microdissected in Biological Technology

Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.

Gene expression profiling of material isolated by microdissection has become a very popular method for analyzing cellular behavior in a micro scale and is used in a wide range of research and clinical applications. ...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

... Int., 125 : 205-11. 6. Siwoski, A., et al., (2002) An Efficient Method for the Assessment of DNA Quality of Archival microdissected Specimens , Molecular Pathology, 15 (8): 889. 7. van Beers E., et al., (2006) A multiplex PCR predictor for aCGH success of FFPE samples ....

Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

... by bioanalyzer (Agilent Technologies, Palo Alto, CA) (Figure 1C). Eight to ten micrograms (8-10 g) of aRNA was obtained from CapSure caps filled with microdissected cells, enough for quadruple hybridizations for each sample, using 2 g of aRNA per hybridization. ...

Microgenomic Expression Profiling

...chieve meaningful expression array data from minute samples with confidence. This application note provides a workflow for laser-cut and laser-capture microdissected pure cell populations through to microarray-based expression analysis using state-of-the-art technologies. Introduction ...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...stic film mounted on optically transparent LCM caps over selected regions. 30 proximal aortic sections from each apolipoprotein E-deficient mouse were microdissected in regions immunostained positive for CD68/Macrosialin and with morphologically-identifiable cells having the characteristic foamy appearance. Approxi...

GenomiPhi amplification of FFPE tissue DNA for array CGH

...uman Genetics Annual Meeting, Toronto, Canada, 2004.

6. Cardoso, J. et al . Genomic profiling by DNA amplification of laser capture microdissected tissues and array CGH. Nucleic Acids Res. 32 , e146 (2004).

7. Wang, G. et al . DNA amplification method tolerant to sampl...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

...on was done by histological routine procedures. A section was stained with Hematoxylin & Eosin and used as reference. Cells were microdissected from an immediate corresponding unstained 10 m thick section using a micromanipulator (Narishige, Japan). For the aspiration of ...

RNA from LCM Samples

... then be extracted from these microdissected cells to analyze their gene e...target structure (dentate gyrus). (C) microdissected dentate gyrus adhered to LCM cap. The thermoplasti...ure 2. Electropherogram of RNA from microdissected Dentate Gyrus. RNA was extracted from...

Protect RNA and Improve Target Cell Identification

...ith Acridine Orange will fluoresce on the cap after microdissection, making it easy to assess whether the cells were effectively microdissected and retrieved. If tissue morphology is slightly damaged, Acridine Orange may also provide better contrast than Cresyl Violet. ...

Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis

...obal expression profiles or specific gene expression levels from microdissected samples is becoming increasingly important for understanding bio... Ambion scientists have now optimized its use for RNA recovery from microdissected samples. The improved kit shows a significant increase in total ...
microdissected in Biological Products

CapSure HS LCM Caps with 32 ExtracSure devices and 12 PrepStrips (Alignment Tray and Incubation Block required, not included) from Arcturus, now part of Molecular Devices

Description:...rapid extraction of populations of pure cells from tissue sections and cytology samples with Laser Capture Microdissection. Ensure the Integrity of microdissected Cells for Downstream Molecular Analysis All CapSure LCM Caps have a patented transfer film bonded to the lower cap surface. Using the PixCell, Auto...
Company:Arcturus, now part of Molecular Devices

CapSure Macro LCM Caps - Bulk Pack from Arcturus, now part of Molecular Devices

Description:...rapid extraction of populations of pure cells from tissue sections and cytology samples with Laser Capture Microdissection. Ensure the Integrity of microdissected Cells for Downstream Molecular Analysis All CapSure LCM Caps have a patented transfer film bonded to the lower cap surface. Using the PixCell, Auto...
Company:Arcturus, now part of Molecular Devices

CapSure Macro LCM Caps from Arcturus, now part of Molecular Devices

Description:...rapid extraction of populations of pure cells from tissue sections and cytology samples with Laser Capture Microdissection. Ensure the Integrity of microdissected Cells for Downstream Molecular Analysis All CapSure LCM Caps have a patented transfer film bonded to the lower cap surface. Using the PixCell, Auto...
Company:Arcturus, now part of Molecular Devices

CapSure HS LCM Caps Starter Pack with Alignment Tray, Incubation Block, 24 ExtracSure devices and 12 PrepStrips from Arcturus, now part of Molecular Devices

Description:...rapid extraction of populations of pure cells from tissue sections and cytology samples with Laser Capture Microdissection. Ensure the Integrity of microdissected Cells for Downstream Molecular Analysis All CapSure LCM Caps have a patented transfer film bonded to the lower cap surface. Using the PixCell, Auto...
Company:Arcturus, now part of Molecular Devices

CapSure HS LCM Caps - Bulk Pack (Alignment Tray and Incubation Block required, not included) from Arcturus, now part of Molecular Devices

Description:...rapid extraction of populations of pure cells from tissue sections and cytology samples with Laser Capture Microdissection. Ensure the Integrity of microdissected Cells for Downstream Molecular Analysis All CapSure LCM Caps have a patented transfer film bonded to the lower cap surface. Using the PixCell, Auto...
Company:Arcturus, now part of Molecular Devices
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