miRNA

Harnessing miRNA natural gene repressors for anticancer therapy

Michel Sadelain and colleagues, at Memorial Sloan-Kettering Cancer Center, New York, have developed a new approach to modulate the expression of genes for therapeutic purposes, and used this to mediate effective anticancer therapy in mice. Small, noncoding RNA molecules known as miRNAs are powe...

Scientists discover new role for miRNA in leukemia

COLUMBUS, Ohio Scientists here have found that mini-molecules called micro-RNA may play a critical role in the progression of chronic myeloid leukemia (CML) from its more treatable chronic phase to a life-threatening phase, called blast crisis. Furthermore, they discovered an entirely new func...

Sigma-Aldrich Announces mirPremier(TM) microRNA Isolation Kit for miRNA Purification

Provides researchers tool for 50% faster miRNA preparation with no detectable large RNA and further supports Sigma's commitment to the rapidly developing area of RNAi ST. LOUIS, Nov. 1 /PRNewswire-FirstCall/ -- Sigma-Aldrich(TM) (Nasdaq: SIAL) is pleased to introduce ...

Study Shows a Link Between Schizophrenia & Genetic Mutations

...cattered over the genome. Components required for mirna processing and/or function have been implicated in...ase. In this study, the sequences of 59 mirna genes on the X-chromosome were determined in 193 m...ome. Eight ultra-rare variants in eight distinct mirna genes were detected in 4% of the men with schizoph...

Study shows promise for new cancer-stopping therapy

...ver, the research reported in Cell suggests that mirna gene delivery may be a clinically viable therapy w... number of miRNAs, including miR-26a. By combining mirna technology developed at Johns Hopkins University w... for the first time that therapeutic delivery of a mirna in an animal can result in tumor suppression, with...

John P. Cogswell, Ph.D., receives 2009 Alzheimer Award

...the recipient of the 2009 Alzheimer Award presented by the Journal of Alzheimer's Disease in recognition of his outstanding work, "Identification of mirna changes in Alzheimer's disease brain and CSF yields putative biomarkers and insights into disease pathways" (J Alzheimers Dis 14: 27-41, 2008) by J.P....

Olivier Voinnet awarded 2009 EMBO Gold Medal

...croRNAs also control antibacterial defense by targeting genes involved in hormone responses and that, in response, bacteria produce suppressors of the mirna pathway. "Yet another illustration of the never ending molecular arms race between hosts and parasites." "Olivier Voinnet's discoveries represent t...

InteRNA Technologies and Radboud University Nijmegen Medical Centre to Develop microRNA-based Therapeutics for Prostate Cancer

...teRNA Technologies B.V. InteRNA Technologies B.V. actively explores and exploits opportunities to translate its unique collection of miRNAs and mirna discovery and validation technologies into successful diagnostic, prognostic and therapeutic applications. The company's primary focus is to unravel t...

MicroRNA undermines tumor suppression

.... RELEVANCE: The study reports the first time a mirna has been shown to directly affect the p53 protein ...nes and Development reports the first time that a mirna has been shown to directly affect the p53 gene, al... certain gene is translated into a protein. When a mirna matches with and binds to a given messenger RNA co...

JCI online early table of contents: Dec. 1, 2008

... EDITOR'S PICK: Harnessing mirna natural gene repressors for anticancer therapy Michel Sadelain and col...iRNAs are powerful natural repressors of gene expression. In the study, the mirna miR-181a was harnessed to segregate expression of genes in immune cells kno...

miRNA in Medical Technology

New Small and Medium Sized Enterprises Join TI Pharma by Signing Two New Projects

...ding to the consortium members. Development of novel anti-angiogenic mirna based therapeutics "Conventional cancer treatment such as surgery, r... the development of (anti-cancer) therapeutics based on angiostatic miRNAs. mirna is utilized as a therapeutic modality and advanced nanoparticle delivery sy...

InteRNA Technologies and VU University Medical Center to Develop microRNA-Based Diagnostics and Therapeutics for Cancer

...on, InteRNA will apply its unique lentiviral-based mirna overexpression library in multi-parametric, high-t... and head and neck cancer. In addition, diagnostic mirna profiles will be developed through next-generation...ng and functional screens based on our proprietary mirna library in the diverse research projects. This res...

Rosetta Genomics and M. D. Anderson to Develop a MicroRNA-based Diagnostic Test to Identify Risk of Recurrence of Lung Cancer

...omarkers, we have an opportunity to develop a novel test that will assist clinicians and patients to better manage this type of cancer." "The use of mirna with existing genomic and proteomic information will help us achieve the goal of personalization of the treatment," said Gordon Mills, co-director of ...

Exiqon Forms Research Collaboration With M. D. Anderson Cancer Center

...ver microRNA biomarkers. Specifically, the project focuses on identifying mirna expression signatures associated with relapse and progression of breast can...cent initiatives, has positioned itself as a significant player in applying mirna as the key biomarker in cancer diagnostics. For more information, pleas...

Northwest Biotherapeutics Conducting Ovarian Cancer Clinical Trial With DCVax(R)-L at University of Pennsylvania

...ancer vasculature that can be targeted for early detection and therapy; and the identification of several gene alterations in ovarian cancer including mirna genes. The clinical research activities of the Center focus on developing individualized therapy approaches to patients with ovarian cancer, combini...

Alnylam and Stanford University Scientists Discover New Role for microRNAs (miRNAs) in T Cell Biology and Immunity

...r efforts to explore therapeutic opportunities for mirna antagonists," said Dr. Muthiah Manoharan, Vice Pre...at occur naturally within all mammalian cells. The mirna molecules are encoded by the cell's own genes, giv...iRNAs. There are believed to be over 250 confirmed mirna genes in the human genome and there are many other...

miRNA in Biological News

Genetic Engineering & Biotechnology News reports on advances in miRNA

New Rochelle, NY, February 29, 2008 miRNA research is now one of the most interesting areas in the life science world, reports Genetic Engineering and Biotechnology News (GEN). Scientists around the globe are studying the effects of over- or underexpression of specific miRNAs on the development a...

Research uncovers clues to virus-cancer link

...e cancerous growth through alteration of cell miRNA, since certain kinds of mirna are responsible for putting the 'brakes' on uncontrolled cell growth. Di...vided with the consent of Kaposi's sarcoma patients and found that specific mirna biomarkers accurately identify stages of tumor progression. They found tha...

NYU Langone Medical Center researchers find micro RNA plays a key role in melanoma metastasis

...tional Academy of Sciences ( PNAS ), suggest that mirna silencing to counteract or attack this mechanism m...is, this is the first study to examine the role of mirna in metastatic melanoma. "Melano...ses. Dr. Hernando's lab found a mirna is over-expressed in metastatic melanoma cell line...

Scientists identify novel inhibitor of human microRNA

..." he says. "The problem is, to date, no one had been able to show that such mirna inhibitors exist." Huang and his colleagues developed a method to identify inhibitors of mirna pathways in live human cells. The researchers created screening assays, or ...

Blood 'fingerprints' for cancer

...g and colleagues are the first to comprehensively characterize entire blood mirna profiles of healthy subjects and patients with lung cancer, colorectal canc...nd diabetes, ruling out contamination. They propose that the specific serum mirna expression profiles they identified constitute fingerprints for cancer and ...

Yale scientists show that a microRNA can reduce lung cancer growth

...orldwide. This study indicates a direct role for a mirna in cancer progression and introduces a new paradig...ncer. We believe this is the first report of a mirna being used to a beneficial effect on any cancer, l...acks research group initially discovered the let-7 mirna in C. elegans , a tiny worm used as a model syste...

Less can be more, for plant breeders too

...uced into indica varieties by several years of breeding. With an artificial mirna targeting the Eui1 messenger RNA, the researchers at the International Rice...authors web site, at http://wmd2.weigelworld.org. Similarly, the artificial mirna vector is provided free of charge to colleagues. ...

Making sense of antisense microRNAs

...oth reveal that antisense transcription of the Hox mirna locus, miR-iab-4, generates the novel mirna precursor mir-iab-8, which is processed into activ... knock out of miR-iab-4 reveals the existence of a mirna transcribed from the opposite strand. Furthermore,...

University of Pennsylvania researchers zero in on the tiniest members in the war on cancer

...lls, the team also found that Myc was directly attaching to the DNA at the mirna genes. "This was further evidence that the decrease in mirna levels was directly due to the action of Myc," says Chang said. "This stu...

Mechanism for the in-vivo transport of siRNA

... group of small RNAs, in the same way. The same mechanism ought to work for mirna inhibitors as well. Since mirna is increasingly suspected of occupying a decisive role in gene regulation i...

miRNA in Biological Technology

Genetic Engineering & Biotechnology News reports on advances in miRNA

New Rochelle, NY, February 29, 2008 miRNA research is now one of the most interesting areas in the life science world, reports Genetic Engineering and Biotechnology News (GEN). Scientists around the globe are studying the effects of over- or underexpression of specific miRNAs on the development a...

Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells

Prevalence and Roles of miRNAs More than 200 miRNAs have been cloned and sequenced from human and mouse samples. Each miRNA apparently regulates multiple genes, and miRNAs are thought to r...

Prepare siRNA and miRNA Probes in Just 1 Hour

mir Vana miRNA Probe Construction Kit Prepare your probe in less than 1 hour Ideal for siRNA/miRNA studies Synthesize high specific activity RN...

Precursor miRNAs for Successful miRNA Functional Studies

Pre-miR miRNA Precursor Molecules miRNA target site identification and validation Screening for miRNAs that regulate target gene expression Screening for miRNAs that affect a cellular process ...

Novel MicroRNA Array Technology for Sensitive miRNA Profiling

Uncover Critical Regulatory Pathways by Analyzing a Complete Set of Human and Mouse miRNAs The miRNA gene family contains hundreds of translation regulators, yet their presence and function has only re...

Get the miRNA OutPlant Applications

Patricia Powers & Emmanuel Labourier, Ambion, Inc Quantitative recovery of small RNA such as small interfering RNA (siRNA) and microRNA (miRNA) requires optimized RNA isolation procedures [1...

Isolate It All: siRNA miRNA Total RNA Native Protein

Emmanuel Labourier , Ambion, Inc Isolate total RNA and native protein from the same sample Enrich for small RNAs ( Ideal for assessing siRNA or miRNA expression and processin...

Detecting miRNA & siRNA

Sensitive Solution Hybridization Assay For Small RNAs In the past few years, interest in the identification, detection, and use of small RNA molecules has exploded. This interest has primarily stemmed from two ...

Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform

Ambion has developed a complete line of research tools for the analysis of microRNAs (miRNAs). Recently, we introduced the mir Vana miRNA microarray platform (consisting of the mir Vana miRNA Labeling Kit and the mir Vana miRNA Probe Set...

CapitalBio Corporation and VigeneTech Inc. Have Announced the Launch of a Fully Integrated Microarray Solution Platform

...microarray scanners. The LuxScan(TM) system with MicroVigene(TM) is well-suited for diagnostic and high-throughput DNA, protein, antibody, and mirna array applications. The embedded image acquisition control, combined with the synchronized image analysis of the LuxScan/MicroVigene platform, offers ...

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miRNA in Biological Products

mirVana miRNA Isolation Kit from Ambion

Description: siRNA and miRNA Isolation Traditional mRNA isolation procedures were developed to efficiency recover mRNA and can result in the loss of substantial amounts of small RNAs. Ambions mirVana miRNA Isolation Kit purifies all RNA species (including small RNAs), both single- and double-stranded. Through...
Company:Ambion

mirVana™ miRNA Bioarray Essentials Kit from Ambion

Description: The mir Vana miRNA Bioarray Essentials Kit provides all the necessary materials and reagents for hybridization and post-hybridization washing of the mir Vana miRNA Bioarrays when using a miRNA preparation that has been labeled using Ambions mir Vana miRNA Labeling Kit. The kit also includes a po...
Company:Ambion

miRCURY LNA Northern Blotting Detection probes from Exiqon

Description:... known miRNAs in invertebrates, vertebrates and plants, as annotated in the mirna Registry at The Wellcome Trust Sanger Institute. Custom miRCURY detection p...les Superior to DNA probes Fast get results in a few hours Detect mirna in as little as 2.5 g total RNA $250 - 415 (dependent on labelling)...
Company:Exiqon

miRCURY LNA in situ Detection probes from Exiqon

Description:...e specific and sensitive detection of miRNAs. Specific in situ detection of mirna is possible in whole mounts, thin sections, single cells and in formalin-fi...n invertebrates, vertebrates and plants, as registered and annotated in the mirna Registry at The Wellcome Trust Sanger Institute. Custom miRCURY detection p...
Company:Exiqon

microRNA Expression Profiling - Comprehensive Service from LC Sciences

Description:...chnology and proprietary probe design, which enable highly sensitive and specific direct detection of miRNAs (1-3). We have standard arrays for mature mirna of all species available in the latest version of the Sanger miRBase database (Release 8.2). Our service is comprehensive and includes small RNA enric...
Company:LC Sciences

miRCURY LNA Array microarray slides from Exiqon

Description:...h standard microarray slides and microarray analysis instruments and software. Hybridization and wash buffers are included. Save time - no need for mirna enrichment Preserve your sample - works on less than 1 g total RNA / sensitivity Reliable results - single base discrimination ...
Company:Exiqon

Small Non-coding RNA Discovery Microarray - Comprehensive Service from LC Sciences

Description:...ed data, representative and original images of the array, and raw data. We offer additional products and services including combined arrays containing mirna and/or miRNA* probes and in-depth analysis for multiple data sets. The optional data analysis may include multi-array normalization, t-test, ANOVA, Fa...
Company:LC Sciences

miRNA in Biological Dictionary

Solution hybridization

...hours exposure) ... Solution Hybridization Improves Sensitivity ... Ambion makes this solution hybridization assay available as the mirVana mirna ... The probe concentration in the hybridization solution should be 10 ng/mL if the ... Pour off the hybridization solution into the radi...

MicroRNA

...oads and offers. Sign up here ... MicroRNA or mirna are small RNA molecules 21–23 nucleotides in lengt...roRNA panels and custom assays for multiplexing mirna targets ... The FlexmiR™ MicroRNA Panels liquid ...tion ... TargetmiR Infobase of microRNA Targets. mirna target ... TaqMan® MicroRNA Assays. Custom Ta...

Mature transcript

...rimary transcript appears to be more prevalent than the mature mRNA in the ... Reporter constructs to monitor primary transcript and mature mirna activities. ... primary miR-23a transcript and the activity of mature miR-23a simultaneously, 3 ... ... indicate that GH may stabilize mature ...

Gene amplification

...... Scan: ==> Gene - Amplification News ... © Copyright Gene Logic (An Ocimum Biosolutions Company). All Rights Reserved. Gene , Exon & mirna Expression. Overview. Samples. Logistics. Genotyping & Copy Number ... Brief and Straightforward Guide: What Is Gene Amplification ? ... Ge...

Expression clone

...arget ... Expression Clone Construction ... mirna & Target Clones - Largest collection of vector based expression ready mirna clones for 15,00 human, mouse and rat genes. 3...ession Cloning Kits. Library Construction Kits. mirna Cloning Kit (Micro RNAs) ... Competent Cells ( C...

Expression Vector

...hoosing an expression vector is your host system. ... pGB vector has been optimized for suppressing expression of target ... pMIR-REPORT™ mirna Expression Reporter Vector System ... This validated reporter system contains two mammalian expression vectors (see figure at right) ... Th...

Alu sequence

... PLoS ONE: an inclusive, peer-reviewed, open-access resource from the PUBLIC LIBRARY OF SCIENCE. ... Hotspots in the Alu sequence are targets for mirna seeds ... particular Alu sequence on one or both of your number 16 chromosomes. ... will determine how rare this Alu sequence is in the hu...