Biologists develop genome-wide map of miRNA-mRNA interactions
Researchers at New York University's Center for Comparative Functional Genomics and the University of California, Berkeley have used computational analyses to predict a genome-wide map of microRNA (miRNA) targets in the animal model organism, Caenorhabditis elegans (C. elegans). MicroRNAs bind to messenger RNA (mRNA) in a specific section, called 3'UTR, and are known to regulate them. Parts of th...miRNA-mediated silencing of mRNAs
Dr. Elisa Izaurrralde and colleagues (EMBL; Max-Planck-Institute for Developmental Biology-Tuebingen) provide new mechanistic insight into gene silencing by miRNAs MicroRNAs (miRNAs) are a novel class of small regulatory RNAs that silence the expres...Stability of mRNA/DNA and DNA/DNA duplexes modulates mRNA transcription
The distribution of the four nucleotides along the DNA sequence encodes the genetic information in living systems. However, do nucleic acids possess other attributes that contribute to their biological functions? Recent work of a team led by Stoyno Stoynov, working at the Bulgarian Academy of Sciences, suggests that thermodynamic stability of DNA/DNA and RNA/DNA duplexes influences mRNA transcrip...mScript mRNA Production System from EPICENTRE Biotechnologies
Description:Create 100% capped transcripts with the translation boosting Cap 1 structure. mScript offers higher yields of 100% capped (versus 40% to 80% capped using co-transcriptional systems) eukaryotic mRNA messages in about 2 hours. mScript also features the Cap 1 structure, which has been shown to be up to 50% more efficient in in vivo tranlsation studies. mScript is the only commecially...Quantikine mRNA Base Kit from R&D Systems
Description:...Comparison of Different Methods of mRNA Quantitation
<TABLE width="96%" border=0 align="left" cellPadding=8 cellSpacing=1> Nuclease Protection Assays <b...Measuring siRNA-mediated knockdown of IL-8 mRNA
By Genospectra, Inc., 6519 Dumbarton Circle, Fremont, CA 94555. A critical step in siRNA-mediated gene expression knockdown studies is the validation of expression inhibition. The QuantiGene Reagent System and the Molecular Devices LMax II microplate luminometer were used to measure IL-8 mRNA in PMA-induced HeLa cells, following siRNA t...Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes
The following protocol was optimized from a protocolusing 35S-labeled RNA probes [1, 2]. In research studies,it enables detection of the expression of rare mRNAs inthe cardiovascular system (e.g., of the proinflammatorycytokine granulocyte macrophage colony stimulating...Gene-Expression Analysis of TP, DPD, and TS Using the LightCycler mRNA Quantification KitsPLUS
In human cells, the enzymes thymidin phosphorylase(TP), dihydropyrimidine dehydrogenase (DPD), and thymidylatesynthase (TS) play a pivotal role in the metabolismof pyrimidines. In cancer disease medical care, thecytotoxic compound 5-fluorouracil (5-FU) is the standard...Bacterial mRNA Isolation, Fast and Easy
(patent pending) is currently the only commercially available bacterial mRNA purification kit. It uses a novel strategy that rapidly removes >95% of the 16S and 23S rRNA from total RNA of and other bacterial sp...