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loxP in Medical News

Genome Annotation by Mutation

... used short pieces of DNA known as loxP. Pieces of loxp act like signposts saying, Cut the DNA between these two signposts. In the new method, loxp is inserted in a chromosome on one mouse, and in a...ls. When the mice are bred, an offspring mouse has loxp DNA on two sites on the same chromosome and also h...
loxP in Biological News

Mutating the entire genome

... used short pieces of DNA known as loxP. Pieces of loxp act like signposts saying, “Cut the DNA between these two signposts.” In the new method, loxp is inserted in a chromosome on one mouse, and in a...ls. When the mice are bred, an offspring mouse has loxp DNA on two sites on the same chromosome and also h...
loxP in Biological Technology

Mammalian Expression Vectors with Interchangeable Selectable Markers

...ed out in vitro by a Cre-mediated recombination of loxp sites, and recombination products are selected ...linearized insertion modules, which are flanked by loxp sites and provide hygromycin, puromycin, or neo...ctors vectors ( Figure 1 ) that contain a loxp site that serves as the acceptor site for...
loxP in Biological Products

* Quick & Easy Conditional Knock Out Kit - loxP from Gene Bridges GmbH

Description: This kit is designed for the fast and simple integration of loxP-sites into large vector plasmids at any position (for the generation of transgenic mouse models). The included loxP-PGK-gb2-neo-loxP cassette is designed to allow neomycin / kanamycin selection in prokaryotic and eukaryotic cells. It ...
Company:Gene Bridges GmbH

FRT flanked, Pro and Euk. Neomycin Selection Cassette plus loxP site(FRT-PGK-gb2-neo-FRT-loxP) from Gene Bridges GmbH

Description: The FRT-PGK-gb2-neo-FRT-loxP template is designed to allow kanamycin/neomycin selection in prokaryotic and eukaryotic cells, respectively. It combines a prokaryotic promoter (gb2) for expression of kanamycin resistance in E.coli with a eukaryotic promoter (PGK) for expression of neomycin resis...
Company:Gene Bridges GmbH

loxP flanked Chloramphenicol Selection Cassette (loxP-cm-loxP) from Gene Bridges GmbH

Description:...iciency than the generally used Tn5 promoter. A synthetic polyadenylation signal terminates the chloramphenicol expression. The cassette is flanked by loxp sites for later excision by Cre recombinase. Unique Not I and Xho I sites flank the cassette for convenient cloning with restriction sites. Using t...
Company:Gene Bridges GmbH

loxP flanked, Pro and Euk. Neomycin Selection Cassette (loxP-PGK-gb2-neo-loxP) from Gene Bridges GmbH

Description:...gene (PGK) is used as eukaryotic promoter. A synthetic polyadenylation signal terminates the kanamycin/neomycin expression. The cassette is flanked by loxp sites for later excision by Cre recombinase. Unique Not I and Xho I sites flank the cassette for convenient cloning with restriction sites. Using t...
Company:Gene Bridges GmbH
loxP in Biological Dictionary

Recombinase

... bacteriophage that catalyzes site-specific recombination of DNA between loxp sites. The enzyme does not require any energy cofactors and Cre-mediate...uickly reaches equilibrium between substrate and reaction products. The loxp recognition element is a 34 base pair... Recombinase . From Wikipe...
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