isothiocyanate

Also in the Jan. 27 JNCI

...relations office, ncipressofficers@mail.nih.gov , 301-496-6641 Benzyl isothiocyanate Induces Apoptosis in Pancreatic Cancer Cells Benzyl isothiocyanate (BITC), a compound found in cruciferous vegetables, induces apoptosis in hu...

Detecting Cold, Feeling Pain: Study Reveals Why Menthol Fresh

...licit the sensation of burning pain through activation of the same receptor on sensory neurons. Most recently, they identified the receptor of isothiocyanate compounds, which constitute the pungent ingredients in such plants as wasabi and yellow mustard. In response to high temperatures, the receptor produc...

Turmeric And Cabbage effective against prostate cancer

... A new research conducted at the State University of New Jersey (Rutgers) revealed that curry spice turmeric in combination with phenethyl isothiocyanate (PEITC) is very effective in the treatment of prostate cancer.// PEITC is found abundantly in cauliflower, cabbage, watercress, winter cress, bro...

Broccoli Found to Block Cancer Progression In Both Animal and Human Studies

...kly (as cancer does) because of insertion of a gene known to be involved in cell growth and regulation. Laboratory tests showed that the derivative of isothiocyanate significantly pushed the human lung cells to commit 'suicide', compared to cells that did not have the gene, suggesting that its use may stop fast gro...

isothiocyanate in Medical Products

Testosterone EIA kit

Description:...om binding proteins, making it available for antibody binding. Testosterone then equilibrates with an anti-Testosterone antibody linked to fluorescein isothiocyanate (FITC) and alkaline phosphatase labelled Testosterone. An anti-FITC antibody bound to magnetic particles then separates the testosterone/testosterone ...
Company:Immunometrics

Estradiol EIA kit

Description:...m binding proteins, making it available for antibody binding. Estradiol then reacts successively with an anti-Estradiol antibody linked to fluorescein isothiocyanate (FITC) and alkaline phosphatase labelled Estradiol . An anti-FITC antibody bound to magnetic particles then separates the estradiol/estradiol label-an...
Company:Immunometrics

isothiocyanate in Medical Dictionary

Fluorescein

... Fluorescein has an absorption maximum at 494 nm and emission. Fluorescence ... Fluorescein is also known as a color additive ... Fluorescein isothiocyanate ... Remove excess fluorescein from the reaction mixture by gel exclusion ... If the fluorophore is not fluorescein , choose wavelengths that ar...

isothiocyanate in Biological News

Detecting cold, feeling pain: Study reveals why menthol feels fresh

... elicit the sensation of burning pain through activation of the same receptor on sensory neurons. Most recently, they identified the receptor of isothiocyanate compounds, which constitute the pungent ingredients in such plants as wasabi and yellow mustard. In response to high temperatures, the receptor produc...

Curry and cauliflower could halt prostate cancer

...of prostate cancer, particularly when combined with certain vegetables. The scientists tested turmeric, also known as curcumin, along with phenethyl isothiocyanate (PEITC), a naturally occurring substance particularly abundant in a group of vegetables that includes watercress, cabbage, winter cress, broccoli, Bru...

isothiocyanate in Biological Technology

Rice rolls out new nanocars

...e are winners. The ones that don't are either fluorescing molecules sitting by themselves or nanocars stuck in park. The dye tetramethylrhodamine isothiocyanate had the added attraction of emitting a polarized signal. Since dye molecules tended to line up with the chassis, the researchers could always tell wh...

Laser Capture Microdissection of Cells Labeled with Enhanced Green Fluorescent Protein

...or visualization. EGFP fluoresces green when excited by light of 488 nm and may be visualized by epifluorescence microscopy using standard fluorescein isothiocyanate filters (Chalfie et al., 1994) under wavelengths that are not deleterious to living tissues. Since EGFP consists of ~200 amino acid residues, it may b...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

...est tubes and stored at -80C until use. RNA Isolation Total RNA was isolated from 50 mg of tissue by the guanidinium isothiocyanate method, using a ToTALLY RNA kit (Ambion) according to the manufacturers protocol. To remove genomic DNA contamination, isolated ...

Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms

...s from the tautog (Tautoga onitis), a commercially and recreationally important Atlantic finfish, using a commercial guanidinium isothiocyanate DNA extraction kit (PureGene, Gentra). For the oyster, a ~400 bp fragment of the mitochondrial 16S RNA subunit was amplified usi...

Hands-free Tissue Disruption No Homogenization Needed

... kits (Figure 2A): single reagent, phenol-based lysis solution (Kit 1), followed by a glass fiber filter treatment, and a guanidinium isothiocyanate (GITC), glass filter-based method (Kit 2). However, RNA yields from the MELT protocols were up to three times greater (Figure 2B...

Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit

...yledon) and Zea mays (monocotyledon). The technology of the Perfect RNA Kit is based on cell lysis with a chaotropic guanidinium isothiocyanate solution, resulting in the rapid inactivation of cellular RNases. The RNA in the lysate is then selectively bound to a specially...

Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel

... Introduction The classic RNA isolation procedures are based on a mono-phasic solution of phenol and guanidinium isothiocyanate solutions, followed by organic extraction and alcohol precipitation of the RNA.1 TRIzol is the commercial version of this popula...

Total RNA Isolation Protocol 1,2,3

... cells, add 200 l 4 M Guanidinium isothiocyanate Solution (4M Guanidinium Isothioc...olayer, add 200 l 4 M Guanidinium isothiocyanate Solution directly to each well of... Add 100 l of the 4 M Guanidinium isothiocyanate Solution directly to each well of ...

New Microarray Labeling Kit for Preparing cDNA Probes

...be preparation. The presence of contaminants, such as carbohydrates, can increase background fluorescence following hybridization. The guanidinium isothiocyanate method used in the StrataPrep total RNA miniprep kit 2 has been used successfully in this application. The human -actin and additional targ...

isothiocyanate in Biological Products

Mouse Anti-IFN-alpha2 Monoclonal Antibody, Fluorescein isothiocyanate (FITC) Conjugated, Clone 225.C from eBioscience

Description: Fluorescein isothiocyanate (FITC) mouse anti-human IFN-alpha2 50 ug...
Company:eBioscience

Mouse Anti-IFN-alpha2 Monoclonal Antibody, Fluorescein isothiocyanate (FITC) Conjugated, Clone 225.C from eBioscience

Description: Fluorescein isothiocyanate (FITC) mouse anti-human IFN-alpha2 25 tests...
Company:eBioscience

Custom Production Service - Conjugation from AbD Serotec

Description:... Custom Production Service - Conjugation Alkaline Phosphatase Conjugation Horseradish Peroxidase Conjugation Biotin Conjugation Fluorescein isothiocyanate Conjugation R. Phycoerythrin Conjugation Rhodamine Conjugation Allophycocyanin Conjugation Alexa Fluor 488 Conjugation Alexa Fluor 647 Conjugatio...
Company:AbD Serotec

TRIzol Reagent from Invitrogen

Description:... TRIzol Reagents are ready-to-use monophasic solutions of phenol and guanidine isothiocyanate suitable for isolating total RNA DNA and proteins (16)....
Company:Invitrogen

TRIzol LS Reagent from Invitrogen

Description:... TRIzol Reagents are ready-to-use monophasic solutions of phenol and guanidine isothiocyanate suitable for isolating total RNA DNA and proteins (16)....
Company:Invitrogen

isothiocyanate in Biological Dictionary

Microfilament

....wikipedia.org /wiki/ Microfilament " ... Microfilament Actin cytoskeleton of mouse embryo fibroblasts , stained with Fluorescein isothiocyanate - phalloidin Microfilaments (or actin filaments microfilament n. Any of the minute fibers located throughout the cytoplasm of cells, composed...