Histidine-tagged Protein Purification Resin from R&D Systems
Description:Request Info...New gravity-flow column gives faster and simpler purification of histidine-tagged proteins
A. Bergh, M. Carlsson, A. Karlsson, and H. Lindgren GE Healthcare, Uppsala, Sweden Histidine-tagged proteins* can be quickly and simply purified on His GraviTrap columns prepacked with Ni Sepharose 6 Fast Flow without the need for a pump or purification system. The protein binding capacity (approx. 40 mg/column) is among the hi...Addition of imidazole during binding improves purity of histidine-tagged proteins
GPC Biotech AG, Martinsried, Germany The purity of histidine-tagged proteins* purified by metal chelate affinity chromatography can often be improved by optimizing the imidazole concentration in the sample and binding buffer to achieve a balance between purity and yield of the protein of...