LC-MALDI and MALDI-MS/MS for the Analysis of Complex Peptide Mixtures
... protein) was digested using trypsin. 5 uL of the digest supernatent (500 fmol per protein) was loaded onto a 300 um i.d. x 15 cm C18 column (LC Packi...ed for LCMALDI analysis using an SCX trap. 5 L of the digest mixture (500 fmol per protein) was loaded onto a C18 column and prepared as described above...ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)
...ase reagent is shown in Figure 2. The light emission in the presence of 100 fmol ATP/well (upper plot) was maximal within 1 seconds and remained at that lev...e Lmax microplate luminometer gives a detection limit of approximately 0.02 fmol ATP/well and a dynamic range of 5 logs with the ENLITEN ATP assay system....Novel MicroRNA Array Technology for Sensitive miRNA Profiling
...set corresponding to all known human and mouse miRNAs--allowing thorough analysis Detects as little as 0.1 fmol miRNA--allowing analysis of low amounts of starting material The miRNA array protocol (Figure 1), ...