Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion
Transactivators
Lisa Hexdall Li Xu Alan Greener Mary Buchanan John Bauer Chao-Feng Zheng Stratagene HLR-CREB, HLR-Elk1, and HLR-cJun, cell lines for...
Cell lines expressing recombinant aequorin and a G-protein coupled receptor for functional screening
Many G-protein coupled receptors (GPCRs) trigger, upon binding of an agonist, a transient increase in intracellular calcium concentration. This variation acts as an internal secondary messenger and is an important modulator of many physiological mechanisms (reviewed by Rink (1990), Tsunoda (1993) and by Santella & Carafoli (1997)). Measure...
Use of FlashPlate Technology for In Vitro Measurement of [35S]-GTPγS Binding in CHO Cells Expressing the Human 5-HT1B Receptor
SmithKline Beecham Pharmaceuticals, UK FlashPlate is a 96-well white polystyrene microplate with scintillant-coated wells designed for highvolume, in-plate radiobinding assays. This study investigates the use of FlashPlate as a platform for the measurement of G-protein act...
Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
SmithKline Beecham Pharmaceuticals, UK Due to recent technologies in the pharmaceutical industry that have resulted in an increase in the production of potential drug candidates, it has become vital that screening assays are high throughput. FlashPlate technology from PerkinElmer Life Sciences has pro...