Endonuclease

Enigma Diagnostics Announces Patent On Real-Time Monitoring of Amplification Reactions Upheld In Entirety Following Opposition From BD at European Patent Office

...ellectual property exclusively licensed to Enigma Diagnostics by the Secretary of State for Defence. The technology involves the use of a thermostable endonuclease to cut a probe that recognises a specific sequence in the target amplification product. The system may be applied to a wide variety of molecular diagn...

Endonuclease in Biological News

Just a little squeeze lets proteins assess DNA

... Seven years ago, she figured out the structure of a protein called HincII that snips up DNA. The protein is a type of enzyme called a restriction endonuclease and comes from Haemophilus influenzae bacteria. Since that time, Horton has been trying to learn how HincII interrogates the DNA to find the right...

Scientists spy enzyme that makes us unique

... a process at the heart of human individuality. The discovery of the T7 endonuclease 1 enzymes structure was made by using x-ray crystallography techniques. The...nds join together at random points in their sequence(1). Enzymes such as T7 endonuclease 1 are then responsible for severing the strands at this junction, thus crea...

Scientists identify specific enzymes that make meningitis hard to fight

...are therefore unaffected by the body's defences. The research team found that the two enzymes essential to the bacteria's repair mechanism are AP endonuclease and 3'-phosphodiesterase. Contrary to the scientists' expectations, these two enzymes carry out separate functions in the DNA repair process. Pro...

Removing DNA repair gene causes metabolic syndrome

... by their efforts to clone and crystallize homologs to the Escherichia coli endonuclease VIII gene. E. coli endonuclease VIII is part of a pathway of enzymes involved in repairing DNA damaged by f...

Endonuclease in Biological Technology

Transgenomic Awarded SBIR Grant to Support Development of SURVEYOR(R) Endonuclease Adaptor-ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation

OMAHA, Neb., Nov. 20 /PRNewswire-FirstCall/ -- Transgenomic (OTC Bulletin Board: TBIO) today announced that it has been awarded a Phase I Small Business Innovation Research (SBIR) award by the National Science Foundation (NSF) to support the development of its proposed SURVEYOR Endonuclease Adapto...

Transgenomic Introduces SURVEYOR(R) Endonuclease Adaptor-Ligated Libraries (SEAL) for Determining Whole Genome Sequence Variation

High Throughput Technology Identifies DNA Variations Between a Reference Genome and Test Genome OMAHA, Neb., June 17 /PRNewswire-FirstCall/ -- Transgenomic (OTC Bulletin Board: TBIO) today announced that it is developing SURVEYOR Endonuclease Adaptor-ligated Libraries (...

Frost & Sullivan Awards Precision Biosciences for Technology Innovation and Molecular Design Process in the Genomics Market

...research in order to identify best practices in the industry. About Precision Biosciences Precision's mission is to utilize its engineered endonuclease technology to become the world leader in the field of genomic molecular biology. Precision's proprietary Directed Nuclease Editor(TM) (DNE) technol...

Polycystic Kidney Disease Assay at Cornell University Uses Transgenomic's Surveyor Nuclease & WAVE Technology

...ent that we have invested in these technologies." Technical Information Transgenomic's SURVEYOR Nuclease is a proprietary mismatch-specific endonuclease that efficiently detects any mismatches in double-stranded DNA and cleaves at the site of DNA mutations. It identifies all base substitutions, inserti...

Alfacell's ONCONASE(R) and R-Amphinase Show Anti-Tumor Activity in Chronic Lymphocytic Leukemia and Acute Myeloblastic Leukemia Cells

...ic effect of ONCONASE and R-Amph was evident after 48 and 72 hours of treatment, respectively. Synergistic activity was seen in the combination of the endonuclease plus either purine analogue. The combination of doxorubicin with ONCONASE or R-Amph demonstrated an increase in pro-apoptotic activity when compared t...

GenomiPhi amplification of FFPE tissue DNA for array CGH

...e; PicoGreen™ dsDNA Assay Kit (Invitrogen) HyperLadder I DNA ladder (BioLine) Dpn II restriction endonuclease (New England BioLabs)

Methods DNA extraction from FFPE tissue slides Genomic DNA was isolated ...

Detection of Apolipoprotein Gene Variants by DGGE Using the DCode System

...A using a temperature cycler (Ericomp Inc., San Diego, CA). Both oligonucleotide primers had GC clamps. 2 Following restriction endonuclease digestion, the samples were subjected to DGGE. The amplification reactions were performed in 50 mM Tris-HCl, pH 9 (at ...

Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells

...5. Zimmerly, S., Guo, H., Eskes, R., Yang, J., Perlman, P. S. and Lambowitz, A. M., A group II intron RNA is a catalytic component of a DNA endonuclease involved in intron mobility, Cell, 83 , 529-538 (1995). Biolistic is a registered trademark of E.I.DuPont deNemours ...

Separation and Comparison of Proteins From Virulent and Nonvirulent Strains of the Fish Pathogen Flavobacterium psychrophilum, Using a 2-D Electrophoretic Approach

...ent 1 (40 mM Tris, pH 8.0). Bacteria were lysed by rapid freezethawing (10 cycles), and were vortexed between cycles for 12 min. endonuclease was added to a concentration of 150 U/ml and samples were incubated at room temperature for 30 min to digest DNA. Samples were t...

Recombinant HIV Reverse Transcriptase

...MV RT. Ambion's HIV RT is a high quality enzyme that has been tested for contaminating endonuclease and exonuclease activity. The enzyme is available in a convenient 500 U size. A 10X Reaction Buffer is supplied with the enzyme....

Endonuclease in Biological Products

Gelzyme enzyme from Invitrogen

Description:...The remaining carbohydrate molecules will not interfere with subsequent DNA manipulation. DNA isolated with Gelzyme enzyme can be used for restriction endonuclease digestions ligations and transformations. Gelzyme enzyme is ideal for isolating DNA from low melting temperature agarose gels. It is provided with Gel...
Company:Invitrogen

High Molecular Weight DNA Markers from Invitrogen

Description:...inear double-stranded DNA from 9 to 48 kb on low-percentage agarose gels. The 13 bands consist of 8.3- to 48.5-kb fragments generated from restriction endonuclease digests of Lambda DNA (cIind1ts857 Sam7). Ethidium bromide staining causes the four bands identified by an asterisk (8271 bp 8612 bp 12220 bp and 1939...
Company:Invitrogen

Plant DNAzol Reagent from Invitrogen

Description:...Reagent is specifically formulated for the isolation of genomic DNA from plants. Genomic DNA isolated with DNAzol Reagents can be used for restriction endonuclease digestion Southern blot analysis molecular cloning and PCR (12)....
Company:Invitrogen

Topoisomerase I (Vaccinia) from Molecular Cloning Laboratories (MCLAB)

Description:...e degree of supercoiling of naturally occurring DNA Detecting mutant plasmids that differ in length by only one base-pair Increasing restriction endonuclease digestion of resistant DNA substrates by "unwinding" the DNA coils to expose restriction sites ...
Company:Molecular Cloning Laboratories (MCLAB)

GenElute Agarose Spin Columns from Sigma-Aldrich

Description:... The maximum gel slice weight is approximately 200 mg. The purified DNA can be used in most downstream applications such as ligation, PCR, restriction endonuclease digestion, labeling and hybridization....
Company:Sigma-Aldrich

RNase Inhibitor from Applied Biosystems

Description:...mbinant enzyme used to inhibit RNase activity. It does not contain DNase or endonuclease activity. Inhibits RNase activity, preventing degradation of RNA template Lacks DNA endonuclease activity for better product yield ...
Company:Applied Biosystems

DNA Exonuclease III , E. coli from EPICENTRE Biotechnologies

Description:...r more in length, single-stranded DNA, or on thioester-linked nucleotides. The enzyme also has intrinsic RNase H, 3'-DNA phosphatase, and apurinic DNA endonuclease activities....
Company:EPICENTRE Biotechnologies

Taq DNA Polymerase, 500 U (2 x 250 U) without dNTP from Bioneer, Inc.

Description:...ed in the amplification and sequence testing of DNA through PCR. The quality of the Taq DNA polymerase has been tested by the Activity Test, SDS-Page, endonuclease Test, and others....
Company:Bioneer, Inc.

DNase I (RNase-free) (2 U/l) from Ambion

Description:... DNase I (RNase-free) (E.C. 3.1.21.1) is a nonspecific endonuclease that degrades double-and single-stranded DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides w...
Company:Ambion

EZ-96 DNA Methylation Kit - Deep Well from Zymo Research

Description:...to10 l provided elution buffer. *Input DNA: 500 pg - 2 g DNA per treatment with 200 - 500 ng being optimal. *Sample Sources: Plasmid, genomic, and endonuclease digested DNAs. *Equipment: Microcentrifuge (EZ DNA Methylation Kit) centrifuge w/ microplate carriers (EZ-96 DNA Methylation Kit)....
Company:Zymo Research

Endonuclease in Biological Definition

Restriction enzyme

... A restriction enzyme (or restriction endonuclease ) is an enzyme that cuts double-stranded DNA . The enzyme makes two incisions, one through each of the phosphate backbones of the double helix ...

Endonuclease in Biological Dictionary

Endonuclease

Endonucleases are enzymes that cleave the phosphodiester bond within a ... Restriction endonucleases (Restriction Enzymes) cleave DNA at specific sites, ... Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae" ... new site-specifi...

AP endonuclease

Apurinic/apyrimidinic ( AP ) endonuclease is an enzyme that is involved in the DNA base excision repair pathway (BER). Its main role in the repair of damaged or mismatched nucleotides in DNA is to create a nick in the... Base excision repair (BER) is a cellular mechanism that repairs dam...

S1 nuclease

... S1 nuclease is an endonuclease that is active against single-stranded DNA and RNA molecules. It is five times more active on DNA than RNA. Its reaction products are oligonucleotide...

Saccharomyces cerevisiae

...yces cerevisiae are single-celled fungi which that ... Saccharomyces cerevisiae cdc9, a structural gene for yeast DNA ligase which ... endonuclease alpha from Saccharomyces cerevisiae shows increased activity on ... synteny blocks of current Saccharomyces cerevisiae genes. We ... Sacch...

Ribonuclease

...can be divided into endoribonucleases and exoribonucleases, and comprise several sub-classes within the EC 2.7... Ribonuclease A (RNase A) is an endonuclease that cleaves single-stranded RNA. Bovine pancreatic RNase A is one of the classic model systems of protein science. Ribonuclease summary with ...

Retrotransposon

...uman LINE-1 retrotransposon induces DNA damage and apoptosis in cancer cells ... Jr, Boeke JD: Human L1 retrotransposon encodes a conserved endonuclease required ... inferred from 33 non-LTR retrotransposon clones. ... retrotransposon elements should be further investigated ... Morrison HG (2...

Restriction map

... X06403. ... ... a textual map showing the positions of restriction endonuclease cut sites. ... Restriction Map supports the entire IUPAC alphabet and several genetic codes. ... Restriction mapping . The endonuclease Ceu-I recognizes a 19 bp sequence. ... Draw a restriction map of the f...

Restriction enzymes

...s cleave foreign DNA, thus eliminating ... Restriction enzymes can be isolated from bacterial cells and used in the ... Finds restriction endonuclease cleavage sites in DNA sequences. ... Maps sites for restriction enzymes , a.k.a. restriction endonucleases, in DNA sequences. ... With more...

Restriction enzyme

...on enzymes , methylases, the microorganisms from which they have been isolated, recognition sequences, cleavage sites, ... Finds restriction endonuclease cleavage sites in DNA sequences. ... Maps sites for restriction enzymes , a.k.a. restriction endonucleases, in DNA sequences. ... A restri...

Restriction site

...plorer's Restricted Sites Zone? ... Because we are talking about the Restricted Sites zone you will likely want to ... Finds restriction endonuclease cleavage sites in DNA sequences. Supports linear and circular DNA and provides several ways to sort and filter output. CLC bio offers a wide r...