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electrocompetent in Biological Technology

Successful PCR amplification and subcloning of a GC-rich DNA fragment

...te screening, and ampicillin at 30 g/ml. A second set of ligation reactions were carried out, and 3 l aliquots used to transform electrocompetent DH5-a E. coli. After transformation, bacteria were grown at 37C for 40 minutes before plating as above. White colonies derived f...

Ustilago maydis

...0 Fax +49 6421 282 8971 e-mail: rfischer@mailer.uni-marburg.de Making electrocompetent cells: 1. Inoculate 50 ml of YEPS with a primary culture of Ust...

Saccharomyces cerevisiae

...USA Making electrocompetent cells: ...oporation of cells: Add <5 l (0.1 g) DNA to 65 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Staphylococcus epidermidis

...208 Making electrocompetent cells: ...oom temperature. Add 1-2 l plasmid DNA (100-500 ng) to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Streptococcus salivarius

...804 Making electrocompetent cells: ... Thaw cells on ice. Add 1 g plasmid DNA to 100 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Staphylococcus aureus

...208 Making electrocompetent cells: ...oom temperature. Add 1-2 l plasmid DNA (100-500 ng) to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several times. ...

Saccharomyces cerevisiae

...USA Making electrocompetent cells: ...oporation of cells: Add <5 l (0.1 g) DNA to 65 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Salmonella typhimurium

...477 Making electrocompetent cells: ...ls: Add 2 l plasmid DNA (20 pg to 20 ng) to 40 l of electrocompetent cells. Homogenize by gently mixing with pipette se...

Nocardia asteroides

...227 Making electrocompetent cells: ...on of cells: Add 2 l (0.1 g) plasmid DNA to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several ...

Pseudomonas putida

....de Making electrocompetent cells: ...tion of cells: Add 4 l plasmid DNA (1 g) to 40 l of electrocompetent cells. Homogenize by gently mixing with pipette several ...
electrocompetent in Biological Products

CopyRight pEZ BAC BamHI Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pEZ BAC Blunt Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pSMART VC BamHI Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pSMART VC Blunt Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pEZ BAC BamHI Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pEZ BAC Blunt Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pSMART VC Blunt Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pSMART VC BamHI Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

Transformance™ competent cell preparation service from Lucigen

Description:...217;s unrivalled expertise in producing competent cells. Lucigen’s Transformance Custom Competent Cell Service offers: Chemically competent or electrocompetent cells High transformation efficiencies Fast turnaround Quality control performance testing Large or small quantities Great value! ...
Company:Lucigen
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(Date:10/22/2014)... By Maureen Salamon ... -- A crucial part of conventional in vitro fertilization (IVF) ... can instead take place in a device inside the vagina, ... Colombia contend that the device, called an INVOcell, might sharply ... could also make the technology more accessible to those who ...
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(Date:10/18/2014)... In a sample of patients with undiagnosed, suspected genetic ... associated with a higher molecular diagnostic yield than traditional ... JAMA . The study is being released to ... meeting. , Exome sequencing, which sequences the protein­coding region ... genetic material present in a cell or organism), has ...
(Date:10/17/2014)... . Why do ... to reproduce? And why are there two sexes anyway? These ... of the research journal Molecular Human Reproduction published ... Bielefeld University Bielefeld has compiled this special issue on sperm ... to copulate with several males in quick succession – chimpanzees ...
(Date:10/17/2014)... available in German . ... few drugs. When treating overdoses, doctors are often limited to ... if there is a combination of drugs involved. So what ... swallows his grandmother,s pills? ETH professor Jean-Christophe Leroux from the ... an answer to this question. "The task was to develop ...
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