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electrocompetent in Biological Technology

Successful PCR amplification and subcloning of a GC-rich DNA fragment

...te screening, and ampicillin at 30 g/ml. A second set of ligation reactions were carried out, and 3 l aliquots used to transform electrocompetent DH5-a E. coli. After transformation, bacteria were grown at 37C for 40 minutes before plating as above. White colonies derived f...

Ustilago maydis

...0 Fax +49 6421 282 8971 e-mail: rfischer@mailer.uni-marburg.de Making electrocompetent cells: 1. Inoculate 50 ml of YEPS with a primary culture of Ust...

Saccharomyces cerevisiae

...USA Making electrocompetent cells: ...oporation of cells: Add <5 l (0.1 g) DNA to 65 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Staphylococcus epidermidis

...208 Making electrocompetent cells: ...oom temperature. Add 1-2 l plasmid DNA (100-500 ng) to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Streptococcus salivarius

...804 Making electrocompetent cells: ... Thaw cells on ice. Add 1 g plasmid DNA to 100 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Staphylococcus aureus

...208 Making electrocompetent cells: ...oom temperature. Add 1-2 l plasmid DNA (100-500 ng) to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several times. ...

Saccharomyces cerevisiae

...USA Making electrocompetent cells: ...oporation of cells: Add <5 l (0.1 g) DNA to 65 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Salmonella typhimurium

...477 Making electrocompetent cells: ...ls: Add 2 l plasmid DNA (20 pg to 20 ng) to 40 l of electrocompetent cells. Homogenize by gently mixing with pipette se...

Nocardia asteroides

...227 Making electrocompetent cells: ...on of cells: Add 2 l (0.1 g) plasmid DNA to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several ...

Pseudomonas putida

....de Making electrocompetent cells: ...tion of cells: Add 4 l plasmid DNA (1 g) to 40 l of electrocompetent cells. Homogenize by gently mixing with pipette several ...
electrocompetent in Biological Products

CopyRight pEZ BAC BamHI Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pEZ BAC Blunt Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pSMART VC BamHI Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pSMART VC Blunt Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pEZ BAC BamHI Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pEZ BAC Blunt Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pSMART VC Blunt Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pSMART VC BamHI Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

Transformance™ competent cell preparation service from Lucigen

Description:...217;s unrivalled expertise in producing competent cells. Lucigen’s Transformance Custom Competent Cell Service offers: Chemically competent or electrocompetent cells High transformation efficiencies Fast turnaround Quality control performance testing Large or small quantities Great value! ...
Company:Lucigen
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