Navigation Links
electrocompetent in Biological Technology

Successful PCR amplification and subcloning of a GC-rich DNA fragment

...te screening, and ampicillin at 30 g/ml. A second set of ligation reactions were carried out, and 3 l aliquots used to transform electrocompetent DH5-a E. coli. After transformation, bacteria were grown at 37C for 40 minutes before plating as above. White colonies derived f...

Ustilago maydis

...0 Fax +49 6421 282 8971 e-mail: rfischer@mailer.uni-marburg.de Making electrocompetent cells: 1. Inoculate 50 ml of YEPS with a primary culture of Ust...

Saccharomyces cerevisiae

...USA Making electrocompetent cells: ...oporation of cells: Add <5 l (0.1 g) DNA to 65 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Staphylococcus epidermidis

...208 Making electrocompetent cells: ...oom temperature. Add 1-2 l plasmid DNA (100-500 ng) to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Streptococcus salivarius

...804 Making electrocompetent cells: ... Thaw cells on ice. Add 1 g plasmid DNA to 100 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Staphylococcus aureus

...208 Making electrocompetent cells: ...oom temperature. Add 1-2 l plasmid DNA (100-500 ng) to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several times. ...

Saccharomyces cerevisiae

...USA Making electrocompetent cells: ...oporation of cells: Add <5 l (0.1 g) DNA to 65 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Salmonella typhimurium

...477 Making electrocompetent cells: ...ls: Add 2 l plasmid DNA (20 pg to 20 ng) to 40 l of electrocompetent cells. Homogenize by gently mixing with pipette se...

Nocardia asteroides

...227 Making electrocompetent cells: ...on of cells: Add 2 l (0.1 g) plasmid DNA to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several ...

Pseudomonas putida

....de Making electrocompetent cells: ...tion of cells: Add 4 l plasmid DNA (1 g) to 40 l of electrocompetent cells. Homogenize by gently mixing with pipette several ...
electrocompetent in Biological Products

CopyRight pEZ BAC BamHI Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pEZ BAC Blunt Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pSMART VC BamHI Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pSMART VC Blunt Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pEZ BAC BamHI Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pEZ BAC Blunt Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pSMART VC Blunt Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pSMART VC BamHI Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

Transformance™ competent cell preparation service from Lucigen

Description:...217;s unrivalled expertise in producing competent cells. Lucigen’s Transformance Custom Competent Cell Service offers: Chemically competent or electrocompetent cells High transformation efficiencies Fast turnaround Quality control performance testing Large or small quantities Great value! ...
Company:Lucigen
Other Tags
(Date:7/9/2014)... July 9, 2014 There has been a ... UT Southwestern Medical Center using MyChart, the online, interactive ... results, communicate with their healthcare providers, schedule appointments, and ... of patients actively using MyChart each year ... year increased more than 10-fold, according to a study ...
(Date:7/9/2014)... world have been linked to an emerging fungal ... National Institute for Mathematical and Biological Synthesis (NIMBioS) ... , In a series of mathematical models, ... of internal organs in frogs, could cause extinction ... are exposed to the virus every few years, ...
(Date:7/9/2014)... and have a reputation for being highly toxic, but when ... being being found to offer potential health benefits in a ... dementia. A new compound (AP39), designed and made at the ... by targeting delivery of very small amounts of the substance ... Scientists in Exeter have already found that the compound protects ...
(Date:7/9/2014)... liver is one of the most important organs in ... we utilize our food properly this is its ... from our organism this is its detoxification function. ... unhealthy food all damage the liver. The resulting diseased ... cirrhoses, and life-threatening liver failure. According to the German ...
(Date:7/9/2014)... poor printing on prescription labels handed out by ... errors in taking medication, according to new research ... National Institute for the Blind)., The study, published ... found that labels on prescription medications dispensed by ... for legibility. , By simply following recommended guidelines ...
Breaking Medicine News(10 mins):Health News:MyChart use skyrocketing among cancer patients, UT Southwestern study finds 2Health News:MyChart use skyrocketing among cancer patients, UT Southwestern study finds 3Health News:Study predicts ranavirus as potential new culprit in amphibian extinctions 2Health News:Rotten egg gas holds key to healthcare therapies 2Health News:Telemedicine for patients with chronic liver diseases 2Health News:Telemedicine for patients with chronic liver diseases 3Health News:Most prescription labels fail to meet guidelines, risking dosage errors 2
(Date:7/9/2014)... fungal pathogens pose a greater threat to biodiversity ... of amphibians, bats, corals, bees and snakes. New ... in the prestigious journal Nature reveals ... to a deadly chytrid fungus implicated in global ... important because it is the basis of vaccination ...
(Date:7/9/2014)... Guelph scientist that involved fitting bumblebees with tiny ... neonicotinoid pesticide hampers bees, ability to forage for ... professor in Guelph,s School of Environmental Sciences, and ... July 9 in the British Ecological Society,s journal ... how long-term pesticide exposure affects individual bees, day-to-day ...
(Date:7/9/2014)... fever but often can be defeated with antibiotics and ... beat. Now, scientists have built a new weapon against such ... the journal ACS Applied Materials & Interfaces , their ... of them than medicine alone. , David Leong, Jianping Xie ... wait, undetectable in the human body or in places that ...
Breaking Biology News(10 mins):USF study: Amphibians can acquire resistance to deadly fungus 2Bee foraging chronically impaired by pesticide exposure: Study 2
Other Contents