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electrocompetent in Biological Technology

Successful PCR amplification and subcloning of a GC-rich DNA fragment

...te screening, and ampicillin at 30 g/ml. A second set of ligation reactions were carried out, and 3 l aliquots used to transform electrocompetent DH5-a E. coli. After transformation, bacteria were grown at 37C for 40 minutes before plating as above. White colonies derived f...

Ustilago maydis

...0 Fax +49 6421 282 8971 e-mail: rfischer@mailer.uni-marburg.de Making electrocompetent cells: 1. Inoculate 50 ml of YEPS with a primary culture of Ust...

Saccharomyces cerevisiae

...USA Making electrocompetent cells: ...oporation of cells: Add <5 l (0.1 g) DNA to 65 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Staphylococcus epidermidis

...208 Making electrocompetent cells: ...oom temperature. Add 1-2 l plasmid DNA (100-500 ng) to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Streptococcus salivarius

...804 Making electrocompetent cells: ... Thaw cells on ice. Add 1 g plasmid DNA to 100 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Staphylococcus aureus

...208 Making electrocompetent cells: ...oom temperature. Add 1-2 l plasmid DNA (100-500 ng) to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several times. ...

Saccharomyces cerevisiae

...USA Making electrocompetent cells: ...oporation of cells: Add <5 l (0.1 g) DNA to 65 l of electrocompetent cells. Homogenize by gently mixing with pipette several times....

Salmonella typhimurium

...477 Making electrocompetent cells: ...ls: Add 2 l plasmid DNA (20 pg to 20 ng) to 40 l of electrocompetent cells. Homogenize by gently mixing with pipette se...

Nocardia asteroides

...227 Making electrocompetent cells: ...on of cells: Add 2 l (0.1 g) plasmid DNA to 50 l of electrocompetent cells. Homogenize by gently mixing with pipette several ...

Pseudomonas putida

....de Making electrocompetent cells: ...tion of cells: Add 4 l plasmid DNA (1 g) to 40 l of electrocompetent cells. Homogenize by gently mixing with pipette several ...
electrocompetent in Biological Products

CopyRight pEZ BAC BamHI Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pEZ BAC Blunt Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pSMART VC BamHI Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pSMART VC Blunt Cloning Kit (with electrocompetent cells) from Lucigen

Description: Error-free cloning of BAC (100+kb), fosmid (40kb), and large plasmid insert (10-20kb) DNAs. Greater insert stability than any othe BAC vector. On command 20-50 fold copy amplification for high yields and easy recombinant DNA purification. Available with blue-white screening (pEZ BAC) or transcripti...
Company:Lucigen

CopyRight pEZ BAC BamHI Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pEZ BAC Blunt Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pSMART VC Blunt Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

CopyRight pSMART VC BamHI Cloning Kit (without cells) from Lucigen

Description:...A purification. Available with blue-white screening (pEZ BAC) or transcription-free (pSMART VC) vectors for the highest insert stability. Kits include electrocompetent cells or no cells....
Company:Lucigen

Transformance™ competent cell preparation service from Lucigen

Description:...217;s unrivalled expertise in producing competent cells. Lucigen’s Transformance Custom Competent Cell Service offers: Chemically competent or electrocompetent cells High transformation efficiencies Fast turnaround Quality control performance testing Large or small quantities Great value! ...
Company:Lucigen
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Breaking Medicine News(10 mins):Health News:New research to tackle treatment for lung disease 2Health News:New research to tackle treatment for lung disease 3Health News:Take Action To Protect Your Fellow Citizens 2Health News:Take Action To Protect Your Fellow Citizens 3Health News:Researchers identify potential biomarker for AD 2Health News:Patient Engagement in Hospital Infection Control: Online Toolkit Provides a New Solution 2Health News:North America Carotenoids & Antioxidants (Nutraceuticals) Market is Expected to Reach $812.7 Million in 2018 - New Report by MicroMarket Monitor 2Health News:North America Carotenoids & Antioxidants (Nutraceuticals) Market is Expected to Reach $812.7 Million in 2018 - New Report by MicroMarket Monitor 3Health News:North America Carotenoids & Antioxidants (Nutraceuticals) Market is Expected to Reach $812.7 Million in 2018 - New Report by MicroMarket Monitor 4
(Date:7/25/2014)... wildfires have been raging this summer and some of the ... In this image collected by the Moderate Resolution Imaging ... a swath of smoke has descended over the Great Lakes ... is the fire image from July 23, 2014 (first image ... as it drifts off southeastward. In the image, it ...
(Date:7/25/2014)... Nearly 600 scientists from 25 countries and ... Meeting organized by the Genetics Society of America ... in Seattle. The conference will feature close to ... results on topics including gene expression and regulation, ... technologies, evolution, aging, and a variety of diseases. ...
(Date:7/25/2014)... of Adelaide has opened the way for the development of ... In Australia, annual barley production is second only to wheat ... of the most important diseases of barley. , Senior Research ... of special growths on the cell walls of barley plants ... , The research, by the ARC Centre of Excellence in ...
Breaking Biology News(10 mins):Yeast meeting to showcase latest breakthroughs in genetics and molecular biology 2New hope for powdery mildew resistant barley 2
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