Cold Spring Harbor Protocols features stem cell differentiation, plant RNAi methods

... use of viral vectors, with the Tobacco Rattle Virus (TRV) providing the most robust results. "Virus-Induced Gene Silencing as a Tool for Delivery of dsrna into Plants" outlines a simple procedure for introducing TRV-based vectors into commonly studied plants such as Arabidopsis and tomato. This method ...

Ancient immune defense mechanism is no match for HIV-1

...A silencing is a type of natural immune defense in which sequence-specific RNA degradation follows the recognition of double-stranded RNA (dsRNA). The dsrna is processed by a protein called Dicer, which chops the long strands of RNA into smaller pieces, resulting in the production of an RNA-induced silenci...

dsRNA in Biological Technology

Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit

Although the Silencer siRNA Labeling Kits were developed for labeling siRNA, they can be readily adapted for labeling long dsRNA. The following protocol can be used for t...

Quark Pharmaceuticals Announces Poster Presentations at RNAi Europe 2008

..., submitted by Hagit Ashush, is titled "Tandem and RNAstar molecules for simultaneous silencing several genes," and evaluates the potential of complex dsrna structures targeting two ("tandem") or three ("RNAstar") different genes in vitro. The multigene nature of pathogenesis for different diseases raises ...

Nastech Assigns Intellectual Property Related to the Development of RNA-Based Therapeutics to its Subsidiary, MDRNA, Inc.

...mplexes of Nucleic Acids and Organic Cations -- Cationic Peptides for siRNA Intracellular Delivery -- Formulations for Intracellular Delivery dsrna -- Compositions and Methods Employing Universal-Binding Nucleotides for Targeting Multiple Gene Variants with a Single siRNA Duplex -- RNAi T...

RNA Interference and Gene Silencing: History and Overview

... factor responsible for PTGS in plants is dsrna (1-3 ). ...Discovered in Nematodes The first evidence that dsrna could lead to gene silencing came from wo...s then that Fire and Mello first injected dsrna a mixture of both sense and antisense st...

Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect

... Using labeled siRNA, we found that the dsrna accumulated in the cytoplasm near the nucleus. Four hours po...e RISC resides. Previous research on RNAi suggests that long dsrna degrades mature (e.g. cytoplasmic) RNA (Montgomery et al., 1...

siRNA Expression Vectors with Selectable Markers

... expression vectors over siRNAs prepared by chemical synthesis, in vitro transcription or RNase III digestion of long dsrna include the ability to perform long term gene silencing studies and eliminating the need to work directly wi...

siRNA Design: It's All in the Algorithm

...of the sense strand becomes bound to the RISC and degrades transcripts with sequence complementary to the sense strand siRNA, or dsrna binding proteins that stimulate antiviral response pathways are more likely to be activated and induce the expression of antivir...

RNAi: Size Does Matter

... Everyone wants to design the dsrna that most efficiently silences the gene of interest. But how does dsrna length correlate with silencing eff...tion in the following experiment. dsrna of different sizes and directed aga...

RNAi: A How To for New Users

...c nuclease Dicer first cleaves the long dsrna into 2123 bp small interfering RNAs (si... a potent antiviral response upon introduction of dsrna longer than 30 bp, researchers transfec...uite simple: A specific dsrna that targets a particular gen...

RNAi in Non-Mammalian Cultured Cells

... Large-scale Transcription of Long dsrna for RNAi Large mass amounts of ready-to-use dsrna for RNAi experiments in non-mammalian systems can ...anscription, nuclease digestion and dsrna clean up. The kit incorporates a single step, high...

dsRNA in Biological Products

RNase III, E. coli from EPICENTRE Biotechnologies

Description:...pecifically digests double-stranded RNA (dsRNA) to dsrna fragments that have 2-base, 3'-overhangs. Complete digestion of dsrna results in dsrna fragments of 12-15 bp. RNase III can digest doubl...
Company:EPICENTRE Biotechnologies

MEGAscript RNAi Kit from Ambion

Description:... The MEGAscript RNAi Kit is optimized for the synthesis of large mass amounts of ready-to-use dsrna for RNAi experiments in non-mammalian systems. The MEGAscript RNAi Kit is based on Ambions patented high-yield transcription technol-ogy and includes ...
Company:Ambion

dsRNA Marker from Abnova Corporation

Description:... The dsrna Marker consists of ten double-stranded RNAs, 10, 2... 100, 200, 300, 400, 500 and 1,000 base pairs. The dsrna Marker is an ideal size marker for determinating s...randed RNAs. A twenty bp RNA band including in the dsrna Marker is adjusted to approximately 25 ng/µl...
Company:Abnova Corporation

ShortCut RNase III from New England Biolabs

Description:...5 bp) interfering RNAs (siRNA) suitable for RNA interference in mammalian cells. 1.3 units (1 l) of ShortCut RNase III is sufficient to convert 1 g of dsrna into siRNA suitable for RNA interference in mammalian cells....
Company:New England Biolabs

TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation

Description:...pression by utilizing sequence-specific, dsRNA-mediated destruction of the target messenger RNA (mRNA). Attempts to induce RNA interference using long dsrna in mammalian cell lines have been met with limited success, due in part to the induction of the interferon response, which results in a general inhibi...
Company:Mirus Bio Corporation

TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation

Description:...pression by utilizing sequence-specific, dsRNA-mediated destruction of the target messenger RNA (mRNA). Attempts to induce RNA interference using long dsrna in mammalian cell lines have been met with limited success, due in part to the induction of the interferon response, which results in a general inhibi...
Company:Mirus Bio Corporation

dsRNA in Biological Definition

RNA

...c material ( genomes ) of some kinds of viruses. Double-stranded RNA ( dsrna ) is used as the genetic material of some RNA viruses and is involved in so... two complementary strands, similar to the DNA found in all "higher" cells. dsrna forms the genetic material of some viruses . In eukaryotes, it may play a ...

Virus classification

...ses (single stranded DNA) 2.2 RNA viruses 2.2.1 Group III - dsrna viruses (double stranded RNA) 2.2.2 Group IV - (+)ssRNA viruses (positiv...pe species: Torque teno virus RNA viruses Group III - dsrna viruses (double stranded RNA) Family Birnaviridae Family Chrysov...

dsRNA in Biological Dictionary

RNA virus

...ely book brings together all of the key recent research on this disparate group of viruses , providing for the first time a single resource reviewing dsrna ... Double-stranded RNA viruses . Retroviruses. Latent Viruses . Lysogeny. Transduction. Viruses ... Either DNA or RNA , never both. DNA vi...

Drosophila

...hila Genome Project ... Drosophila RNAi collections - Whole genome RNAi screening ... Drosophila S2 cells 72 hours after the addition of dsrna to the pebble gene; ... Robo3 extracellular ( Drosophila ) 15G1a(EcR-A) ecdysone receptor (EcR-A) ... rhodopsin Drosophila . 4C71B7. protei...

Dicer

...d get the highest possible score by finding different combinations. ... Home > Web apps >Games> Dicer ... Dicer makes staggered cuts in dsrna to form siRNA duplexes with 3' overhangs, ... Dicer exhibits little sequence specificity for cleavage, though it favors ... Dicer contains a ...