La Jolla Pharmaceutical Announces Positive Interim Antibody Results From Riquent Lupus Phase 3 Trial
...lieve that the greater reductions in antibodies to dsdna observed with these higher doses of Riquent imply ...f treatment with Riquent on reducing antibodies to dsdna in 101 patients by measuring the percent of antibo...ent, the median percent reduction in antibodies to dsdna for Riquent-treated patients compared with placebo...La Jolla Pharmaceutical Provides Update on Interim Antibody Results From Riquent Lupus Phase 3 Trial
...treatment at which time separation between doses was also seen. The analyses assessed the impact of treatment with Riquent on reducing antibodies to dsdna in 101 patients by measuring the percent of antibody reduction from baseline compared with placebo following weekly treatment with 100 mg, 300 mg or 9...Autoimmunity ENA/DNA Screening Panel
Description:...es antigens detected in Auto 4 detecting the four common extractable nuclear antigens (ENA): RNP, Sm Antigen, SS-A (Ro) and SS-B (La) and also detects dsdna autoantibodies. This one assay is a direct substitute for other tests....Chimerix Completes Phase I Study and Initiates a Phase II Multi-dose Clinical Trial for CMX001
...completed a single and multi-dose Phase I study of CMX001 in healthy volunteers. This study supports the further development of the drug for multiple dsdna infections. The Company has initiated the first Phase II multi-dose clinical trial in patients. "The safety and high oral availability demonst...DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)
...ecular Probes, Inc. 4 The latter reagent binds to dsdna with high affinity and becomesfluorescent. The lim...poration. 5 The DNAQuant is specific for linear dsdna (including PCR fragments) and is not affected byco...uce ATP in an amount proportional to the amount of dsdna present, followed by addition of luciferin/lucifer...Amplification Efficiency of TaqMan Gene Expression Assays
...directions. Bound DNA was eluted in a 100 μL volume of elution buffer. Then, 10 μL of the elute was used for picogreen staining (PicoGreen dsdna quantitation kit, Molecular Probes) to estimate cDNA concentration. A 6-log dilution range was generated using 10-fold serial dilutions of t...GenomiPhi amplification of FFPE tissue DNA for array CGH
... Quant-iT™ PicoGreen™ dsdna Assay Kit (Invitrogen) Hype...s given below. DNA was quantitated using PicoGreen dsdna quantitation reagent.1. Add 2 ... for 10 min. Yield was determined by the PicoGreen dsdna method. Suitability for use in Phi29 DNA polymeras...
GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH
...hotometry because the completed reaction contains unused hexamers, deoxynucleotides in addition to amplification product. We used the PicoGreen™ dsdna method for accurate quantitation of the unpurified amplification product. Microarray fabrication and processing The fol...Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye
... for many applications 4 . SGI is a dsdna binding dye that has proven exceptionally useful f... enhanced approximately 1000-fold upon binding dsdna 5 , making it ideal for detection of am...1a) indicates that the concentration of dsdna in each sample rose above background fluorescence ...Prepare siRNA and miRNA Probes in Just 1 Hour
... labeled probe . Complete Kit for Probe Preparation The kit includes enough reagents to prepare 30 dsdna templates and to perform 30 in vitro transcription reactions. Each template construction reaction supplies sufficient template f...Mung Bean Nuclease from Promega
Description:...es the degradation of single-stranded DNA and RNA endonucleolytically to yield 5-phosphoryl terminated products. While the nuclease prefers ssDNA over dsdna by 30,000-fold, at very high concentrations the enzyme degrades double-stranded DNA from both ends (1,2). Mung Bean Nuclease has been used for transcr...DNA Quantitation Kit (Hoechst) from Bio-Rad
Description:...NA or protein. With this kit, DNA is stained with Hoechst 33258 dye, which can be detected with a VersaFluor or other fluorometer. The kit quantitates dsdna in the range of 10 ng per ml to 5 micrograms per ml and contains Hoechst 33258, calf thymus DNA standard, and assay buffer for 750 standard 2 ml react...SYBR Green I nucleic acid gel stain from Molecular Probes (Invitrogen)
Description:...sensitive nucleic acid gel stain that has bright fluorescence when bound to dsdna and low background in gels, making it ideal for detecting dsdna in gels using laser scanners or standard UV transilluminators. SYBR Green I...SYBR Green I nucleic acid gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)
Description:...ally sensitive nucleic acid gel stain has bright fluorescence when bound to dsdna and low background in gels, making it ideal for detecting dsdna in gels using laser scanners or standard UV transilluminators. SYBR Green ...