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dsDNA in Medical Technology

La Jolla Pharmaceutical Announces Positive Interim Antibody Results From Riquent Lupus Phase 3 Trial

...lieve that the greater reductions in antibodies to dsdna observed with these higher doses of Riquent imply ...f treatment with Riquent on reducing antibodies to dsdna in 101 patients by measuring the percent of antibo...ent, the median percent reduction in antibodies to dsdna for Riquent-treated patients compared with placebo...

La Jolla Pharmaceutical Provides Update on Interim Antibody Results From Riquent Lupus Phase 3 Trial

...treatment at which time separation between doses was also seen. The analyses assessed the impact of treatment with Riquent on reducing antibodies to dsdna in 101 patients by measuring the percent of antibody reduction from baseline compared with placebo following weekly treatment with 100 mg, 300 mg or 9...

Human Genome Sciences Reports That a Phase 2 Study of LymphoStat-B Showed Significant Reductions in Disease Activity in Patients with Active Systemic Lupus Erythematosus

...m Week 52 through Week 76 in the 24-week extension phase of the trial." Dr. Ginzler noted that reductions observed in activated B cells and anti- dsdna autoantibodies, as well as the improvements observed in health-related quality of life, were associated with therapeutic response. No increase in infe...
dsDNA in Medical Products

Autoimmunity ENA/DNA Screening Panel

Description:...es antigens detected in Auto 4 detecting the four common extractable nuclear antigens (ENA): RNP, Sm Antigen, SS-A (Ro) and SS-B (La) and also detects dsdna autoantibodies. This one assay is a direct substitute for other tests....
Company:GenBio
dsDNA in Medical Dictionary

B virus

... hepatitis B virus is a special problem for pregnant women. ... Herpes B virus Herpes B Virus Virus classification Group: Group I ( dsdna ) Family: Herpesviridae Subfamily: Alphaherpesvirinae Genus: Simplexvirus ... B : Causes and symptoms Diagnosis Treatment Prognosis Prevention Re...
dsDNA in Biological Technology

Chimerix Completes Phase I Study and Initiates a Phase II Multi-dose Clinical Trial for CMX001

...completed a single and multi-dose Phase I study of CMX001 in healthy volunteers. This study supports the further development of the drug for multiple dsdna infections. The Company has initiated the first Phase II multi-dose clinical trial in patients. "The safety and high oral availability demonst...

Denaturing HPLC (DHPLC)

... There are columns on the market for performing fragment analysis, they include the DNASep Column, the Helix DVB column and the Zorbax Eclipse dsdna Analysis Column. The ion-pairing agent used with either column is triethylammonium acetate (TEAA), which mediates binding of DNA to the sta...

Scalable Automation of the TempliPhi DNA Sequencing Template Amplification and DYEnamic ET Terminator Sequencing Kits on the Sciclone ALH 3000

...Kit (Amersham BioSciences P/N 25-6400-01) DYEnamic ET Terminator Sequencing Kit (Amersham BioSciences P/N US81095) PicoGreen dsdna Quantitation Kit (Molecular Probes P/N P-7589) Deck Layout: For the TempliPhi Kit, the deck layout is shown in Figure 1. Positi...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

...ecular Probes, Inc. 4 The latter reagent binds to dsdna with high affinity and becomesfluorescent. The lim...poration. 5 The DNAQuant is specific for linear dsdna (including PCR fragments) and is not affected byco...uce ATP in an amount proportional to the amount of dsdna present, followed by addition of luciferin/lucifer...

Amplification Efficiency of TaqMan Gene Expression Assays

...directions. Bound DNA was eluted in a 100 μL volume of elution buffer. Then, 10 μL of the elute was used for picogreen staining (PicoGreen dsdna quantitation kit, Molecular Probes) to estimate cDNA concentration. A 6-log dilution range was generated using 10-fold serial dilutions of t...

GenomiPhi amplification of FFPE tissue DNA for array CGH

... Quant-iT™ PicoGreen™ dsdna Assay Kit (Invitrogen) Hype...s given below. DNA was quantitated using PicoGreen dsdna quantitation reagent.

1. Add 2 ... for 10 min. Yield was determined by the PicoGreen dsdna method. Suitability for use in Phi29 DNA polymeras...

GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH

...hotometry because the completed reaction contains unused hexamers, deoxynucleotides in addition to amplification product. We used the PicoGreen™ dsdna method for accurate quantitation of the unpurified amplification product. Microarray fabrication and processing The fol...

Real-Time Quantification of Genomic DNA Using DyNAzyme II DNA Polymerase and SYBR Green I Dye

... for many applications 4 . SGI is a dsdna binding dye that has proven exceptionally useful f... enhanced approximately 1000-fold upon binding dsdna 5 , making it ideal for detection of am...1a) indicates that the concentration of dsdna in each sample rose above background fluorescence ...

Prepare siRNA and miRNA Probes in Just 1 Hour

... labeled probe . Complete Kit for Probe Preparation The kit includes enough reagents to prepare 30 dsdna templates and to perform 30 in vitro transcription reactions. Each template construction reaction supplies sufficient template f...

Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue

... of DNA we strongly recommend cleaning up the samples after amplification. The amplified products can be measured with the PicoGreen dsdna Quantitation Assay (Molecular Probes Inc. Product # P-7589). Another method of detecting the amplified products is spectrophotom...
dsDNA in Biological Products

DNAclear Kit from Ambion

Description:... DNA is eluted with preheated Nuclease-free Water. The DNAclear Kit can be used to remove free nucleotides, oligonucleotides, proteins, and salts from dsdna samples. The DNA that is recovered from the Microfilter Cartridge is ready for any application that requires highly pure DNA including in vitro transc...
Company:Ambion

Mung Bean Nuclease from Promega

Description:...es the degradation of single-stranded DNA and RNA endonucleolytically to yield 5-phosphoryl terminated products. While the nuclease prefers ssDNA over dsdna by 30,000-fold, at very high concentrations the enzyme degrades double-stranded DNA from both ends (1,2). Mung Bean Nuclease has been used for transcr...
Company:Promega

DNA Quantitation Kit (Hoechst) from Bio-Rad

Description:...NA or protein. With this kit, DNA is stained with Hoechst 33258 dye, which can be detected with a VersaFluor or other fluorometer. The kit quantitates dsdna in the range of 10 ng per ml to 5 micrograms per ml and contains Hoechst 33258, calf thymus DNA standard, and assay buffer for 750 standard 2 ml react...
Company:Bio-Rad

SYBR Green I nucleic acid gel stain from Molecular Probes (Invitrogen)

Description:...sensitive nucleic acid gel stain that has bright fluorescence when bound to dsdna and low background in gels, making it ideal for detecting dsdna in gels using laser scanners or standard UV transilluminators. SYBR Green I...
Company:Molecular Probes (Invitrogen)

SYBR Green I nucleic acid gel stain*10,000X concentrate in DMSO**special packaging* from Molecular Probes (Invitrogen)

Description:...ally sensitive nucleic acid gel stain has bright fluorescence when bound to dsdna and low background in gels, making it ideal for detecting dsdna in gels using laser scanners or standard UV transilluminators. SYBR Green ...
Company:Molecular Probes (Invitrogen)

SYBR Green I nucleic acid gel stain*10,000X concentrate in DMSO* from Molecular Probes (Invitrogen)

Description:...ally sensitive nucleic acid gel stain has bright fluorescence when bound to dsdna and low background in gels, making it ideal for detecting dsdna in gels using laser scanners or standard UV transilluminators. SYBR Green ...
Company:Molecular Probes (Invitrogen)
dsDNA in Biological Definition

Virus classification

...lassification by genome type 2.1 DNA viruses 2.1.1 Group I - dsdna viruses (double stranded DNA) 2.1.2 Group II - ssDNA viruses (single str... known. Classification by genome type DNA viruses Group I - dsdna viruses (double stranded DNA) Order Caudovirales (tailed bacterioph...
dsDNA in Biological Dictionary

Polymerase

...nisms, where it functions to ... EPICENTRE develops and manufactures reagents and kits for genomics, proteomics, ... DisplaceAce™ DNA Polymerase . dsdna Conversion Kit for RiboMultiplier™ sRNA ... ...

Intercalation

...ategy to yield well-ordered uniform ... Intercalation of an agent into dsdna leads to lengthening of the double helix. ... After the hydrodynamic studies revealed intercalation of SG into dsdna at low ... Northeastern University professor Mark C. Williams and collea...

Double stranded

...can use to give your hair a ... Personal Care " Hair Care " Braiding " How to Double Strand Twist Your Hair ... High-affinity autoantibodies to dsdna ( double - stranded DNA) detected by the Farr ... The first international standard for antibodies to double stranded DNA. ... Explains arthr...

DNase

...es. ... Fig. 4: Real-time PCR of putative DNase HS site and random coordinates: ... in the presence of Mg2+, DNase I cleaves each strand of dsdna independently, in ... Studies of DNA-protein interactions by DNase I footprinting (1) ... ...

DNA polymerase

...egin a new chain (de novo) ... EPICENTRE develops and manufactures reagents and kits for genomics, proteomics, ... DisplaceAce™ DNA Polymerase . dsdna Conversion Kit for RiboMultiplier™ sRNA ... Invitrogen Life Science > Brands > Product Brands > PlatinumTaq DNA Polymerase ... Once di...

DNA ligase

...t is DNA ligase ? Meaning of DNA ligase medical term. What does DNA ligase mean? ... ligase chain reaction. satellite DNA . Z-DNA ... dsdna fragments in 30 min. T3 DNA ligase was shown to be over 5-fold more efficient ... analysis of T4 DNA ligase showed that the inactive ... Fi...

Bacteriophage lambda

...n for lambda to enter the lytic or lysogenic cycle when it first ... BACTERIOPHAGE LAMBDA . LAMBDA is a tailed bacteriophage with a linear dsdna genome characterized by ... bacterium is protected from superinfection by lambda . ... ... of primary products of bacteriophage lambda recom...
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