Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
1. The aRNA was subjected to cDNA microarray analysis. Amplified RNA was labeled with Cy3 or Cy5 by direct incorporation of Cy3-dUTP or Cy5 dutp
in the reverse transcription reaction using random hexamer as primer. Labeled aRNA from IM and INTER sites were co-hybridized to TwinChip Mouse 6K cDN...
Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
◊ Gene-specific probes
◊ pCRII TOPO cloning kit (Invitrogen Cat. #K460001)
◊ p 33 -labeled dutp
Cold formaldehyde (Sigma)
◊ DEPC-treated PBS (Ambion Cat. #9625)
◊ Triethanolamine (Sigma ...
Microgenomic Expression Profiling
...he second round of amplification, complimentary DNA (cDNA)isgeneratedthroughfirstandsecond strand synthesis, and then used as template for amino-allyl dutp
incorporation during a second round of RNA amplification in preparation for fluorescent dye labeling. aRNA quality is typically assessed at critical s...
Recombinant HIV Reverse Transcriptase
...on) for the indicated times. Reactions were performed using
the manufacturer-supplied reaction buffer in the presence of * 32 -P
and 0.5 U/l ribonuclease inhibitor. The percentage cDNA conversion
was determined by measuring the amount of cDNA made via scintillation
In Situ Cell Death Detection Kit
...by labeling the free 3-OH termini with terminal transferase (TdT), which attaches labeled
nucleotides to all 3OH-ends (TUNEL reaction; TdT-mediated dutp
labeling). This labeling is more sensitive than other methods, and is the method
used by the Roche Applied Science In situ Cell
PCR Polymerases Application Profiles
...ompared to Taq DNA Polymerase.
Defines whether an enzyme system can be used with dutp
dTTP to apply procedures for PCR carry-over prevention using uracil
DNA glycosylase (UNG).
Combine High Yield, Great Accuracy and the
Prevention of Carry-over Contamination by Using
the Novel Expand High FidelityPLUS PCR System
... deliver high yield combined with improved accuracy
to prevent carry-over contamination
when performing amplifications up to ...n every lab.
Until now, carry-over prevention via the incorporation
and subsequent treatment with Uracil-DNA
Glycosylase was only possible w...
Preventing Carry-over Contamination with Uracil-DNA Glycosylase
...to avoid such contamination.
One common strategy is substituting dutp
for dTTP during PCR amplification,
to produce uracil-containing ...es that all PCR-reactions in the lab have to
be carried out with dutp
instead of dTTP.
PCR reaction components
...ering with polymerase
activity and decreasing primer annealing. For prevention of carry-over contamination
a higher concentration of dutp
is usually used in place of dTTP (for details,
Introducing molecular tags, such as digoxigenin
(DIG) or biotin-labeled dutp
into the PCR product, as it was amplified
made PCR an invaluable tool for medical diagnostics. Such labeled
dUTP in Biological Products
CyScribe First-Strand cDNA Labeling System - dUTP with CyScribe GFX Purification Kit from GE Healthcare, formerly Amersham Biosciences
Description: CyScribe First-Strand cDNA Labeling System - dUTP
with CyScribe GFX Purification Kit, 50 reactions. For the generation and purification of Cy3- and Cy5-labeled cDNA.Optimized reagents are packaged together for complete cDNA labeling and purification.Flexible and optimized protocols can be used for ...
Company:GE Healthcare, formerly Amersham Biosciences
Flow Cytometry Kit for Apoptosis from Sigma-Aldrich
Description:...al DNA. Features and Benefits: Greater incorporation of Br-dUTP, resulting in improved detection than found by using biotin- or digoxigenin-conjugated dutp
or by using fluorochrome (fluorescein or BODIPY)-conjugated deoxynucleotides. Packaging: The kit is shipped in two parts, both wet ice. Upon receipt, ...
GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems
Description:...products from previous PCR amplifications in which dutp
has been incorporated, without degrading native nu...s susceptible to degradation involves substituting dutp
for dTTP in the PCR mixture, and pretreating all s...dU-containing DNA,2 ribouracil residues in RNA and dutp
itself are not substrates for UNG.3
Expand High Fidelity PLUS PCR System; Thermostable DNA polymerase mixture from Roche Applied Science
Description:...to Expand High FidelityPLUS Enzyme Blends unique ability to Deliver Higher Yields Improve accuracy for high-fidelity applications, and Incorporate dutp
to prevent carry-over contamination for amplification reactions up to 5 kb....
Company:Roche Applied Science
Thermostable dUTPase from GenScript Corporation
Description:...eading DNA polymerases). It removes contaminating dutp
present in PCR reactions and dNTP solutions. The ...a proofreading DNA polymerase reaction can prevent dutp
misincorporation by maintaining dutp
levels below their inhibitory concentrations despi...
dUTP in Biological Dictionary
.... ... Prokaryotes by Terminal Deoxyribonucleotide Transferase-Mediated dutp
... Terminal deoxyribonucleotide transferase (TdT)-mediated dutp
nick end labeling ... deoxyribonucleotide explanation. ... guanine ...
... The clones from the cpDNA library constructed from Nicotiana tabacum (9) were used as probes. The cpDNA probes were labeled with digoxigenin dutp
by random priming. ... Chloroplast DNA ( cpDNA ) sequence comparisons have been a widely used tool in ... Second, cpDNA intergenic regions ha...