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dNTP in Medical News

TriLink BioTechnologies, Inc. Announces Sales and License Agreement With Glen Research Corporation for CleanAmp(TM) Amidites

... TriLink's CleanAmp(TM) technology offers a less expensive approach to high stringency PCR. CleanAmp(TM) is a unique solution, where the primer or dntp components of the PCR reaction mix, rather than the enzymes are modified. Making the CleanAmp(TM) Amidites available through Glen Research allows inst...
dNTP in Medical Technology

TriLink Awarded a Phase II Grant for a Second Hot Start PCR Project

... $750,000 to continue its investigation of modified dNTPs to improve the fidelity of PCR reactions. Phase I studies demonstrated that TriLink's unique dntp modifications enhanced PCR performance by increasing amplicon formation while reducing off-target amplicon formation. In the second phase of the progr...
dNTP in Biological News

NHGRI seeks DNA sequencing technologies fit for routine laboratory and medical use

...epare genomic DNA for the sequencing process. Jingyue Ju, Ph.D., Columbia University, New York $950,000 (2 years) DNA Sequencing with Reversible dntp and Cleavable Fluorescent ddNTP Terminators This team will develop a hybrid strategy that uses a mixture of chemically modified DNA constituents ca...
dNTP in Biological Technology

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

... The transphosphorylation reaction is catalyzed by the enzyme nucleoside diphosphate kinase (NDPK). In this reaction, the terminal phosphate of the dntp is transferred to ADP to form ATP. The ATP is then measured using a luciferin/ luciferase reagent to produce light in proportion to the amount of ATP ...

Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

... ◊ CapSure HS LCM Caps (Arcturus, Cat. # LCM0214) ◊ FastStart Taq DNA Poymerase (Roche, Cat. # 2 032 926) ◊ dntp Mix PCR Grade (Invitrogen, Cat. # 18427-013) ◊ UltraPure TM Agarose (Invitrogen, Cat. # 15510-027) ◊ UltraPure TM ...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...erse transcriptase enzyme (Invitrogen, Cat. #18064-014) ◊ 10mM dntp mix (Invitrogen, Cat. #18427-013) ◊ Taq DNA polymerase (Invitrog...t strand buffer (50mM Tris-HCl pH 8.3, 75mM KCl, 3mM MgCl2), 5mM DTT, 0.3mM dntp mix, 20U SuperScript II reverse transcriptase enzyme, 2.5U Taq DNA polymera...

Use of the DCode System to Detect the Food-Borne Bacterial Pathogen Listeria monocytogenes

...tion was carried out in a mixture containing 5 ml of 10x cloned Pfu polymerase buffer (Strategene, San Diego, CA), 50 mM of each dntp (Sigma Chemical Co., St. Louis, MO) 0.6 mM of each primer pair, 1 ml containing 2.5 units of cloned Pfu or PfuTurbo (DNA polymer...

Real-Time PCR: General Considerations, Rev A

...hing a new assay: MgCl 2 concentration (3.06.0 mM) dntp concentration (200600 M each dNTP) Increasing the [dNTP] will r...NA Polymerase and 1x PCR buffer (Life Technologies), 0.2 mM each dntp (Advantage Ultrapure dNTPs, Clontech), and SYBR Green I (Molecul...

Detection of K-ras Point Mutations in the Pancreas by Constant Denaturing Gel Electrophoresis Using the DCode System

...lify the K-ras DNA PCR buffer was added to a final concentration of 12 mM Tris/HCl, pH 8.0, 50 mM KCl, 0.1% Nonidet, 0.2 mM each dntp and 2 mM MgCl 2 . The reactions contained in a total volume of 25 l 50 ng of 3-primer (cta ttg ttg gat cat att cg), 100 ng of 5-...

PCR-Grade Nucleotides Larger Pack Sizes Now Available

...P, and dTTP) in separate vials, is now available in a 4 x 125-mol pack size. This larger pack size is sufficient for 6,250 100-l PCRs at a final dntp concentration of 0.2 mM. The 500-mol pack size of single dNTPs provides 25,000 reactions. Dideoxynucleoside Trip...

PCR reaction components

...inimize polymerase error rate. Imbalanced dntp mixtures will reduce Taq DNA Polymerase fidelity. ...increase Mg2+ concentration. Increases in dntp concentration reduce free Mg2+, thus interfering w...with uracil-DNA glycosylase ). The final dntp concentration should be 50500 M (each dNTP). The m...

Trouble-shooting: Low yield

...just the pH to 7.0. Dilute an aliquote of the neutralized NTP or dntp appropriately and read the optical density at the wave lengths g...ct/solutions: Utilization of dNTP's - dntp concentration should be 20-200 M each ...

Pwo DNA Polymerase

... (standard concentration, 2.00 mM) dntp concentration: at least 200 M for each dntp Caution: Lower deoxynucleotide concentrations can increase f...
dNTP in Biological Products

Taq DNA Polymerase, 500 U (2 x 250 U) without dNTP from Bioneer, Inc.

Description: The Polymerase gene from Thermus aquaticus (strain YT1) is cloned and expressed in E. coli, then highly purified to produce Taq DNA polymerase. It is used in the amplification and sequence testing of DNA through PCR. The quality of the Taq DNA polymerase has been tested by the Activity Test, SDS-Pa...
Company:Bioneer, Inc.

MasterAmp Taq PCR Core Kit from EPICENTRE Biotechnologies

Description:... Complete PCR kit. Includes: MasterAmp™ Taq DNA Polymerase, 10X PCR Buffer, MasterAmp™ 10X PCR Enhancer, dntp Mix, 2.5 mM each, 25 mM MgCl2, Enzyme Dilution Buffer, Control Template and Primers Mix...
Company:EPICENTRE Biotechnologies

Taq DNA Polymerase -Klein Taq from AppliChem GmbH

Description:...nucleases/ nickases and exonucleases. *Unit definition: One unit of activity is defined as the amount of enzyme required to incorporate 10 nmoles of dntp into acid-insoluble DNA fraction in 30 minutes at 72 C....
Company:AppliChem GmbH

BioPrime Plus Array CGH Indirect Genomic Labeling Module from Invitrogen

Description:...20C. The BioPrime Plus Systems and Modules contain the Alexa Fluor modified dntp mixes or amine modified dntp mixes and the Alexa Fluor NHS Esters. Store at -20C. The standard BioPrime ...
Company:Invitrogen

BioPrime Array CGH Genomic Labeling System from Invitrogen

Description:...20C. The BioPrime Plus Systems and Modules contain the Alexa Fluor modified dntp mixes or amine modified dntp mixes and the Alexa Fluor NHS Esters. Store at -20C. The standard BioPrime ...
Company:Invitrogen

Thermostable dUTPase from GenScript Corporation

Description:...iosus) maximizes the efficiency of high-fidelity PCR (using proofreading DNA polymerases). It removes contaminating dUTP present in PCR reactions and dntp solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their...
Company:GenScript Corporation
dNTP in Biological Dictionary

Nick translation

... Kit. CGH Reagents. Hybridization Reagents. Microscope ... Nick Translation Kit, dCTP (for use with labeled dCTP) ... Nick Translation Kit, dntp (for use with any radioactive or nonradioactive labeled nucleotide) ... ... Preparation " Nucleic Acid Labeling " Labeling Enzymes " Nick Transl...

Klenow Fragment

...lenow Fragment is also active in many restriction ... Klenow Fragment is also active in many restriction enzyme ... an appropriate 5mM dntp solution and 1–4 units of Klenow Fragment to the reaction ... DNA Polymerase I, Klenow - Fragment from E. coli. AppliChem. 1000U; ... DNA Pol...
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