Navigation Links
dGTP in Biological Technology

Generate High-Quality, Directional Plasmid cDNA Libraries

...I restriction site near its 5 end. First-strand synthesis takes place in the presence of 5-methyl dCTP and nonmethylated dATP, dgtp and dTTP. Second-strand synthesis is then performed using DNA polymerase I, cloned RNase H 3 and nonmethylated dNTPs. The resul...

Randomize Gene Sequences with New PCR Mutagenesis Kit

...CRs). In contrast, to achieve high mutation frequencies with Taq (>4.9-6.6 mutations per kb 2,3 ), it is typical to increase the dgtp concentration (final conditions: 0.5 mM MnCl 2 ; 0.2 mM dATP; 1 mM dCTP/TTP/dGTP), which leads to significant bias in Taqs ...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

... PCR amplification, the PCR product is purified and treated with Pfu DNA polymerase in the presence of dATP. In the absence of dTTP, dgtp and dCTP, the 3 to 5-exonuclease activity of Pfu DNA polymerase removes at least 12 and 13 nucleotides at the respective 3' ends...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

...Following PCR amplification, the PCR product is treated with Pfu DNA polymerase in the presence of dATP. In the absence of dTTP, dgtp and dCTP, the 3 - to 5-exonuclease activity of Pfu DNA polymerase removes 12 to 13 nucleotides at the respective ends of the PC...
Other Tags
(Date:4/17/2014)... the April issue of Immunity , Kevin J. ... Research, says it,s time to take a fresh look ... sepsis, which kills millions worldwide every year, including more ... into the bloodstream to fight an injury or infection ... maintaining good health without inflammation, wounds and infections ...
(Date:4/17/2014)... synthetic biology team has created a new technology ... that could travel the body and selectively target ... cell-based, biological devices that monitor and modify human ... biology. However, no existing technology enabled bioengineers to ... state and respond in a customized fashion. , ...
(Date:4/17/2014)... MAYWOOD, Il. (April 17, 2014) Doctors who ... disease called lymphangioleiomyomatosis (LAM) can face an agonizing ... progression of the disease and help relieve shortness ... lung transplants, and sirolimus can cause potentially fatal ... said pulmonologist Dr. Daniel Dilling, medical director of ...
(Date:4/17/2014)... 16, 2014) The cause of neuronal death in ... proposes that neurons may be mistaken for foreign invaders ... to the way autoimmune diseases like type I diabetes, ... The study was published April 16, 2014, in ... likely controversial, idea in Parkinson,s disease; but if true, ...
(Date:4/17/2014)... By discovering a new mechanism that allows blood to ... UC Irvine and the Salk Institute have opened the ... stroke-induced brain damage. , A complex and devastating neurological ... primary reason for disability in the U.S. The blood-brain ... blood-borne material into the brain, causing the permanent deficits ...
Breaking Medicine News(10 mins):Health News:Feinstein Institute researcher publishes new perspective on sepsis 2Health News:Building 'smart' cell-based therapies 2Health News:Patients with rare lung disease face agonizing treatment dilemma 2Health News:Patients with rare lung disease face agonizing treatment dilemma 3Health News:Is Parkinson's an autoimmune disease? 2Health News:Study IDs new cause of brain bleeding immediately after stroke 2
(Date:4/18/2014)... as soon as they,re planted may be good news for ... In the wild, a plant whose seeds sprouted at the ... than just an insurance policy against late frosts or unexpected ... advantages too: Plants whose seeds put off sprouting until ... in a team of researchers working at the National Evolutionary ...
(Date:4/17/2014)... densely forested and most densely populated quadrant of the ... values and practices. Disturbances associated with those needs, such ... have set the stage for management issues of considerable ... The report Five anthropogenic factors that will radically ... United States was published recently by the ...
(Date:4/17/2014)... for chili peppers. Information about archaeological remains of ancient ... the appearance of words for chili peppers in ancient ... and highlight the value of multi-proxy data analysis. Their ... nine papers presented in a special feature issue of ... on plant and animal domestication edited by Dolores ...
Breaking Biology News(10 mins):Plants with dormant seeds give rise to more species 2Five anthropogenic factors that will radically alter northern forests in 50 years 2Five anthropogenic factors that will radically alter northern forests in 50 years 3Chickens to chili peppers 2Chickens to chili peppers 3
Other Contents