dGTP

Generate High-Quality, Directional Plasmid cDNA Libraries

...I restriction site near its 5 end. First-strand synthesis takes place in the presence of 5-methyl dCTP and nonmethylated dATP, dgtp and dTTP. Second-strand synthesis is then performed using DNA polymerase I, cloned RNase H 3 and nonmethylated dNTPs. The resul...

Randomize Gene Sequences with New PCR Mutagenesis Kit

...CRs). In contrast, to achieve high mutation frequencies with Taq (>4.9-6.6 mutations per kb 2,3 ), it is typical to increase the dgtp concentration (final conditions: 0.5 mM MnCl 2 ; 0.2 mM dATP; 1 mM dCTP/TTP/dGTP), which leads to significant bias in Taqs ...

Expression and Purification of Recombinant Proteins That Have Native Amino Acid Sequence

... PCR amplification, the PCR product is purified and treated with Pfu DNA polymerase in the presence of dATP. In the absence of dTTP, dgtp and dCTP, the 3 to 5-exonuclease activity of Pfu DNA polymerase removes at least 12 and 13 nucleotides at the respective 3' ends...

New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences

...Following PCR amplification, the PCR product is treated with Pfu DNA polymerase in the presence of dATP. In the absence of dTTP, dgtp and dCTP, the 3 - to 5-exonuclease activity of Pfu DNA polymerase removes 12 to 13 nucleotides at the respective ends of the PC...