Generate High-Quality, Directional Plasmid cDNA Libraries
...I restriction site near its 5 end.
First-strand synthesis takes place in the presence of 5-methyl dCTP and
nonmethylated dATP, dgtp
and dTTP. Second-strand synthesis is then performed
using DNA polymerase I, cloned RNase H 3 and nonmethylated dNTPs.
Randomize Gene Sequences with New PCR Mutagenesis Kit
...CRs). In contrast, to achieve
high mutation frequencies with Taq (>4.9-6.6 mutations per kb 2,3 ),
it is typical to increase the dgtp
concentration (final conditions: 0.5
mM MnCl 2 ;
0.2 mM dATP; 1 mM dCTP/TTP/dGTP), which leads to significant bias in Taqs
Expression and Purification of Recombinant Proteins That Have Native Amino
... PCR amplification, the PCR product is purified and treated with Pfu DNA
polymerase in the presence of dATP. In the absence of dTTP, dgtp
the 3 to 5-exonuclease activity of Pfu DNA polymerase removes
at least 12 and 13 nucleotides at the respective 3' ends...
New Yeast Cloning System for Producing Proteins with Native Amino Acid
...Following PCR amplification, the PCR product
is treated with Pfu DNA polymerase in the presence of dATP. In
the absence of dTTP, dgtp
and dCTP, the 3 -
to 5-exonuclease activity of Pfu DNA polymerase removes 12 to 13
nucleotides at the respective ends of the PC...