GenomiPhi amplification of FFPE tissue DNA for array CGH
...il in reference 3. Genomic DNA (500 ng–2 µg) was labeled using
the random priming method in the presence of Cy3- and Cy5-labeled dctp
and Klenow fragment. Cy3- and Cy5 labeled probes were separated on a 2% agarose gel for size analysis and imaged using a Typhoon 8600 Variable Mode Im...
PCR Grade Deoxynucleotides
PCR Nucleotide Mix PLUS
2 x 100l
Premixed solution of dATP, dGTP, dctp
(10 mM each)
and dUTP (30 mM)
Nucleotide mix for UNG-mediated decontamination
Generate High-Quality, Directional Plasmid cDNA Libraries
...-primer that contains an
Xho I restriction site near its 5 end.
First-strand synthesis takes place in the presence of 5-methyl dctp
nonmethylated dATP, dGTP and dTTP. Second-strand synthesis is then performed
using DNA polymerase I, cloned RNase H 3 and n...
Pfu DNA Polymerase as a Hot Start Alternative
... to the multiple cloning region. Primer-extension
reactions were carried out in each enzymes recommended PCR buffer in the
presence of 200 M dATP, dctp
and dGTP, 200 M TTP (including 10 M [ 3 H]TTP;
specific activity of 20.9 Ci/mmole) and 1 g primer-annealed M13 DNA per 100-l
reaction. Reactions w...
...or fluorescence labelling of DNA using ... Nick Translation Kit. CGH Reagents. Hybridization Reagents. Microscope ... Nick Translation Kit, dctp
(for use with labeled dCTP) ... Nick Translation Kit, dNTP (for use with any radioactive or nonradioactive labeled nucleotide) ... ... Preparat...