GenomiPhi amplification of FFPE tissue DNA for array CGH
...il in reference 3. Genomic DNA (500 ng–2 µg) was labeled using the random priming method in the presence of Cy3- and Cy5-labeled dctp and Klenow fragment. Cy3- and Cy5 labeled probes were separated on a 2% agarose gel for size analysis and imaged using a Typhoon 8600 Variable Mode Im...Generate High-Quality, Directional Plasmid cDNA Libraries
...-primer that contains an Xho I restriction site near its 5 end. First-strand synthesis takes place in the presence of 5-methyl dctp and nonmethylated dATP, dGTP and dTTP. Second-strand synthesis is then performed using DNA polymerase I, cloned RNase H 3 and n...Pfu DNA Polymerase as a Hot Start Alternative
... to the multiple cloning region. Primer-extension reactions were carried out in each enzymes recommended PCR buffer in the presence of 200 M dATP, dctp and dGTP, 200 M TTP (including 10 M [ 3 H]TTP; specific activity of 20.9 Ci/mmole) and 1 g primer-annealed M13 DNA per 100-l reaction. Reactions w...