Scientists rid stem cell culture of key animal cells
Tackling a pressing and controversial technical barrier in stem cell biology, scientists at the WiCell Research Institute and the University of Wisconsin-Madison have crafted a recipe that allows researchers to grow human embryonic stem cells in the absence of mouse-derived "feeder" cells, long thought to be a source of potential contamination for the therapeutically promising cells. The...Cancer related gene p53 not regulated as indicated by previous tissue culture research
The cellular cascade of molecular signals that instructs cells with fatally damaged DNA to self-destruct pivots on the p53 tumor suppressor gene. If p53 is inactivated, as it is in over half of all human cancers, checks and balances on cell growth fail to operate, and body cells start to accumulate mutations, which ultimately may lead to cancer. Not surprisingly, the regulation of this vital safe...New classification of eukaryotes has implications for AIDS treatment, agriculture and beyond
New classification conveys important information about the biochemistry and metabolism of disease-causing organisms. The new classification recognizes 6 major clusters of organisms, rather than the 4 traditional Kingdoms. Here are three examples. 1) Pneumocystis, an opportunistic pathogen causing mortality in AIDS patients and immunocompromised individuals, is now known to be a fungus, in...Open Source Biotechnology alliance for international agriculture
CAMBIA & IRRI (The International Rice Research Institute) today announced a majorjoint venture to advance the BiOS Initiative - a new strategy that will galvanize agricultural research focused on poverty alleviation and hunger reduction. The ventureis catalyzed by a 2.55M USD grant to CAMBIA from The Ministry of Foreign Affairs of Norway. The BiOS Initiative ?Biological Innovation for...Corning Proculture Spinner Flask,15L,Vertical S/Arm, No. Of S/A 4,Cent Neck 100mm from Sigma-Aldrich
Description:This CLS number is a new product number, created to easily match Cornings product number. If showing no availability yet, please order under the old Sigma-Aldrich number (Z71,487-9) or contact customer service for assistance. ID clarifier: centre neck 100 mm, No. of sidearm 4 Mfr Desig: Corning 4510-15L...Mitochondria Isolation Kit for Tissue and Cultured Cells from BioChain
Description:Components in this kit are prepared with pure chemicals according to our proprietary technology. Biochains Mitochondria Isolation Kit is designed to isolating intact and active mitochondria in a fast and easy way. One kit is consisted of reagents enough for performing 100 isolations (enriching mitochondria from 10 20 g tissue or from 1x10 cells)....Osteoclasts Culture Kit from B-Bridge International
Description:Osteoclast cells form from a hematopoietic stem cells called monocytes. Osteoclasts resorb bone by attaching to the bone surface and lowering the surrounding pH to an acidic level of around 4.5. The bone mineral is then solubilized and the collagen degraded. Osteoclast differentiation and function is controlled primarily by Macrophage Colony-Stimulating Factor (M-CSF) and Receptor for Activation...Rat Hair Follicle Stem Cell Culture from Celprogen
Description:* Source rat adult and fetal tissue * T25 plated cells $550.00 * T75 plated cells $750.00 * 120 population doublings * This product requires Celprogen Stem Cell complete growth medium M55007-08 and extracellular matrix * E55007-08-T25 or E55007-08-T75...Carrier 3 50 ml tissue culture tubes, aluminium, for HS-4 rotor from Thermo Scientific
Description:Forma Refrigerated Console Orbital Shakers combine high durability and performance with class-leading ergonomics, unequalled programming power. The patented triple-counterbalanced heavy-duty mechanism prevents vibration, allowing the unit to run with heavy out-of-balance loads. The patented HEPA filtration system reduces contamination. The heavy stainless steel interior has coved corners and a...Big Blue Rodent Cell Cultures for Mutagenesis Studies
Rat and mouse embryonic cell lines are useful for in vitro mutagenesisstudies Stratagene Cloning Systems, Inc. The Big Blue rat cell line and the Big Blue transgenic mouse embryoniccell line are useful for in vitro mutagenesis studies. These cell lines are partof the Big Blue transgenic rodent mutagenesis assay system...Simple Isolation of RNA from Tissue and Cultured Cells
Stratagene total RNA miniprep kit efficientlyisolates high-quality total RNA from 10 culturedcells or 10 to 40 mg of tissue and includes an on-column DNA-removal step.Additionally, multiple sa...Easy preparation of total RNA from cultured cells with TRIzol and Eppendorf Phase Lock Gel
Christian Bauer, Oliver Klotzsche, CCS Cell Culture Service GmbH, Falkenried 88, D-20251 Hamburg, Germany, Frank Apostel, Eppendorf AG, Germany The classic RNA isolation procedures are...The QIAGEN Guide to Animal Cell Culture
Welcome to a new series of articles aimed at providing useful hints for culturing animal cells (i.e., cells derived from higher eukaryotes such as mammals, birds, and insects ). This article introduces different types of animal cell cultures. The series...The QIAGEN Guide to Animal Cell Culture
Welcome to the second of a new series of articles aimed at providing useful hints for culturing animal cells. This article describes considerations for working with animal cell cultures as well as information on cell culture cont...Third generation Small pox vaccine from Cell-culture
Richard Greenberg and colleagues compared their Cell culture small pox vaccine// (CCSV) derived from cell culture with calf lymph derived vaccine. Small pox vaccine currently used is a live, attenuated vaccine virus derived from calf lymph. This vaccine produces adverse effects in vaccinated, 15-42 develop serious or life threatening adverse reactions of every million. 1 to 2 people every million...Low Cost Tissue Culture Vaccine For Encephalitis
The Indian Ministry of Health is in search of a new cheaper tissue culture vaccine for Japanese encephalitis. So far the Gorakhpur Government has reported 200 deaths and 700 patients with infection. // Hundreds of people are infected and die every year due to the endemic infection of Encephalitis in Uttar Pradesh. This year the death toll touched 228 in Gorakhpur, with 67 patients were admitted i...Resident Physicians Need More Preparations To Treat Patients From Different Cultures
Research shows that resident physicians are often less prepared to treat patients from different cultures, especially those with practices that differ from certain medical practices // . According to an article in the September issue of JAMA, many resident physicians report a lack of preparedness in caring for patients with specific cultural characteristics, such as those who have beli...Bulgaria Agriculture Minister ban import of poultry from Macedonia and Croatia
Deputy Agriculture Minister Dimitar Peychev said that Bulgaria is to ban the imports of live birds, eggs and poultry meat from Macedonia and Croatia after both countries reported suspected bird flu outbreaks. The first case of bird flu in Europe has been reported from farm// in Balikesir province near the Aegean Sea in Western Turkey. Following the news of bird flu all farm animals and birds are...DNA Underwent Changes Due To Culture
Natural selection is reported to have been responsible for much of the DNA among human beings during the course of the past 50,000 years. The tendency of human beings to live in settlements and cultivate fields can also be traced // to this natural selection process. One way to look for genes that have recently been changed by natural selection is to study mutations called single-nuc...Pathfinder Chlamydia Culture Confirmation Test
Description:The Pathfinder Chlamydia Culture Confirmation provides clear and reliable staining of intracellular inclusions (elementary bodies and reticulate bodies) in tissue culture. The monoclonal antibody used in this kit is directed against the Chlamydia lipopolysaccharide....E. Coli Enrichment Culture Strip
Description:Inquire...