Master molecular switch may prevent the spread of cancer cells to distant sites in the body

...ompletion of the screen. The screen consists of about 15,000 different cdnas (DNA that has been synthesized from messenger RNAs) that each express a dif...ced over wells containing each cDNA and cells that "glowed, indicated those cdnas that produced proteins that promoted the epithelial splicing program. It wa...

cDNAs in Medical Technology

Emisphere Technologies, Inc. Reports Clinical Data on Oral Delivery of GLP-1 and PYY

...ndocrine cells in two principal major molecular forms, as GLP-1(7-36) amide and GLP-1(7-37). The peptide was first identified following the cloning of cdnas and genes for proglucagon in the early 1980s. The main target of action of glucagon-like peptide-1 (GLP-1) is the islet, where the hormone stimulates ...

cDNAs in Biological News

Researchers identify gene linked to aggressive progression of liver cancer

...es relevant to the progression of HCC, including invasion, metastasis, resistance to chemotherapy, the formation of new blood vessels, and senescence. cdnas are complementary DNAs that are generated from mRNAs to analyze gene expression profiles. "AEG-1 also activates multiple intracellular signaling pa...

cDNAs in Biological Technology

Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit

Katrine Verdun, Richard Rouse, and Gary Hardiman BIOGEM, Division of Biology, University of California San Diego, La Jolla, CA 92093-0349 e-mail: ghardiman@ucsd.edu www.biogem.ucsd.edu Introduc...

Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A

... amounts of the initial template molecules than their respective cdnas (the difference in C T ) between the RNA and cDNA in each sample...to the relative expression of GAD67 in the pooled sample. Pooled cdnas were run in every plate simultaneously with experimental samples...

Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A

...Molecular Probes) as described by the manufacturer. First-strand cdnas were synthesized from equal amounts of total RNA (1 g/reaction) ...t 2 cycles earlier than in the mix A-based reactions with macula cdnas for template (Figure 1). In the iQ supermix reactions containing...

Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System

... for up to a 10 million-fold difference in starting template concentrations between 2 PCR targets. Plasmids containing the human cdnas for GAPDH and α-tubulin were multiplexed over varying concentrations. The α-tubulin plasmid was held constant at 10 9 copies ...

Detection of Mutations in the Fas Antigen of Lymphoma Tumors by RT-PCR and Denaturing Gradient Gel Electrophoresis

...troduces a unique restriction site for the enzyme DraI which is not present in the wild type cDNA sequence. Cleavage of the FasIII cdnas with DraI results in an 368 bp fragment when the polymorphic codon is expressed, while this enzyme does not cut the wild type ...

Array Analysis: The Basics

... nucleotides and target specific, oligo dT, or random-sequence primers to create two labeled cDNA populations. The two cdnas are hybridized to two identical arrays. After washing, the hybridized signal on each array is detected and a...

Get the 5'-End

... very efficient at selectively amplifying the shortest targets in a mixed population. RLM-RACE selects cdnas That Contain the True 5'-End In order to add selectivity to RACE, several variations to the basic ...

Expand Long Template PCR System

...ing whole genomes Mapping intron/exon structures by amplifying cdnas Analyzing homologous recombination events Charact...loned sequences carried in lambda-phage Amplifying full-length cdnas or long fragments of cDNA (RACE) Expand Reverse Transcr...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

...ined at a later point in time. However, isolation of full-length cdnas by plaque screening is extremely labor-intensive when the sought.... K., and Martin, G. R. (1988) Rapid production of full-length cdnas from rare transcripts: amplification using a single gene-speci...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

...ined at a later point in time. However, isolation of full-length cdnas by plaque screening is extremely labor-intensive when the sought... M. K., and Martin, G. R. (1988) Rapid production of full-length cdnas from rare transcripts: amplification using a single gene-specifi...

cDNAs in Biological Products

SizeSep 400 Spun Columns from GE Healthcare, formerly Amersham Biosciences

Description:... SizeSep 400 Spun Columns, 10. *SizeSep 400 Spun Columns: For rapid intermediate purification of cdnas > 400 base pairs in length. - *Prepacked with Sepharose CL-4B and pre-equilibrated in distilled water containing 0.15% Kathon CG/IcP Biocide. *...
Company:GE Healthcare, formerly Amersham Biosciences

Custom Splice Arrays from ExonHit Therapeutics Inc.

Description:...vents starting with your gene list. Proprietary algorithms are used to identify splice events and design microarray probes from high quality, spliced cdnas aligned to the human genome. Each splice event contained in a custom SpliceArray is profiled with a patent protected combination of six exon and junct...
Company:ExonHit Therapeutics Inc.

cDNAs in Biological Definition

Molecular genetics

...nockout transgenics and overexpression mapping, cloning, and sequencing gene expression measuring spatial and temporal regulation cdnas imprinting bacterial and phage molecular genetics eukaryotic molecular genetics external links Atlas of Genetics and Cyto...

cDNAs in Biological Dictionary

G1 phase

...erphase, lasting from the end of ... Many G1 - phase -specific mRNAs have been identified from various normal or ... Single-stranded, G1 - phase cdnas were isolated by oligo(dA) chromatography. ... G1 phase information including symptoms, causes, diseases, symptoms, treatments, and other medic...

Directional cloning

... enzymes and lower ... Stratagene's pBluescript II, pCMV-Script and pAD-GAL4-2.1 XR library construction kits offer directional cloning of cdnas and high-efficiency transformation into ... Printable Version E-mail this page. Techology Transfer Staff. Available ... Title: System for the Seq...

CDNA array

...s ten ... hybridization of entire cDNA populations to nucleic acid arrays —a method adopted ... cDNA Expression Arrays include hundreds of cdnas spotted on positively ... cDNA arrays and related types of array technologies have been demontrated to be ... CURRENT ARRAYS BEING PRODUCED...