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cDNA in Medical News

Master molecular switch may prevent the spread of cancer cells to distant sites in the body

...ferase gene and "glow" when it is spliced in the epithelial cell pattern. Cells with this reporter were individually placed over wells containing each cdna and cells that "glowed, indicated those cDNAs that produced proteins that promoted the epithelial splicing program. It was from this screen that ESRP1...

Boston Medical Center/Boston University School of Medicine researcher receives award

...ndidate for hosting the foreign insulin gene. Wolfe is recognized as one of the worlds leading authorities on GIP, and he initially cloned the GIP cdna and ascertained its central role as principal mediator of the enteroinsular axis. This axis functions as the hormonal connection between the intestin...

Cellectricon Launches World's First System for High Throughput RNAi Screening

... genome-wide RNAi screening." About the Cellaxess(R)HT system Cellaxess(R)HT addresses the critical need for high throughput delivery of siRNA and cdna to hard to transfect cells in several cell-based screening assays. Cellaxess(R)HT is the only fully automated system on the market allowing for high t...

Veterinary researchers study poultry virus as human cancer treatment

...wcastle Disease virus to treat prostate cancer. Reverse genetics is the process of generating a recombinant virus from cloned complimentary DNA or cdna copy, explains Subbiah. Through the reverse genetics system, recombinant viruses can be designed to have specific properties that make them attractive...

Researchers Discover Key to Body’s Ability to Detect Subtle Temperature Change

... in real-time in live cells,” Zheng said. In the current experiments, cdna coding for particular subunits is linked to cdna coding for fluorescent proteins and then added to a culture of human embryo...

Researchers Discover Two Proteins Involved In Cellular Destruction Of HIV Virus

...ribonucleic acid or RNA, which gets converted into cdna once it enters the cell. The researchers at Ohio i...air proteins participate in the destruction of HIV cdna in cells," Professor Fishel said. "This process reduces the pool of HIV cdna that can integrate into host chromosomes, thereby ...

Genetic indicates memory

.... They say this study provides a deeper understanding. Rats were put through a standard learning task and then researchers used technology called cdna to screen thousands of genes. They looked at genes in the hippocampus area, a brain region known to be important in learning and memory. The researche...
cDNA in Medical Products

GENios Plus multi label reader

Description:... The DNA Expert: Detection of PCR, cdna and Oligonucleotides The Tecan GENios is an optimal microplate reader for a variety applications that are quantified by Fluorescence, Absorbance or...
Company:Tecan

Genios Multi-Detection Microplate Reader

Description:... The DNA Expert: Detection of PCR, cdna and Oligonucleotides The Tecan GENios is an optimal microplate reader for a variety applications that are quantified by Fluorescence, Absorbance or...
Company:Tecan

MagPrep® Streptavidin Beads

Features:...DNA sequences or mRNAs using appropriate biotinylated probes or primers. The beads are compatible with downstream applications such as sequencing and cdna synthesis, and can also be used for isolation of biotinylated antibodies or other biotinylated targets. ...
Company:EMD Biosciences, Inc.
cDNA in Biological News

New genomic technique uncovers coral transcriptome

... Using a new technique for cdna preparation combined with the latest sequencing methods, researchers have uncovered the larval transcriptome of a reef-building coral ( Acropora mille...

Major international study challenges notions of how genes are controlled in mammals

...tone scientific papers in Nature Genetics and other journals on April 20. FANTOM4, the fourth stage of the Functional Annotation of the Mammalian cdna collaboration, is led by Dr. Yoshihide Hayashizaki of OSC. Dr. Jose Dostie, a biochemist at McGill's Faculty of Medicine joined the FANTOM4 collaborat...

Researchers identify gene linked to aggressive progression of liver cancer

... team found that the expression of AEG-1 gradually increases as the tumor becomes more and more aggressive. Using microarray technology, they analyzed cdna from the tumor cells and determined that AEG-1 modulates expression of genes relevant to the progression of HCC, including invasion, metastasis, resis...

Human genomics in China

...ism detection, as well as for expressed sequence tag (EST) and full-length cdna cloning and sequencing. All these achievements greatly strengthened the Ch... a "two 1%" goal with respect to the genomic sequencing and the full-length cdna identification, respectively, and coordinated the efforts of Shanghai and ...

Genomics of large marine animals showcased in the biological bulletin

...esponse genes in the estuarine anenome Nematostella vectensis, by Adam M. Reitzel, James C. Sullivan, Nikki Traylor-Knowles, and John R. Finnerty cdna sequences for transcription factors and signaling proteins of the hemichordate, Saccoglossus kowalevskii: Efficacy of the EST approach for evolutionar...

Genetic Engineering & Biotechnology News reports on cancer biomarkers

...h.D., harnesses antibodies in patients' serum for the detection of cancer-specific epitopes using peptides selected for IgG binding from phage-display cdna libraries. Other biomarker-related projects covered in the GEN article include those at Sienna Cancer Diagnostics, Geron, Quest Diagnostics Nichol...

Novel antigen-cloning technique may boost efforts to develop a melanoma vaccine

...plementary DNA (cDNA) library. Using the resulting cdna library, the investigators can then express sequen... in recipient cells to find the cell or cells with cdna able to stimulate T cells. The old approach t...transfer them into recipient cells, along with the cdna library. With her new technique, Herlyn expre...

Cracking the egg

...ur during transition to the two-cell stage embryo. The researchers performed a large-scale survey of thousands of fragments of mouse egg cDNA. The cdna fragments are reverse coded from messenger RNA and therefore indicate which genes are being expressed. They identified 5,400 active genes in the fully...

Researchers discover which organs in Antarctic fish produce antifreeze

...h an AFGP gene probe. A clear picture of strong AFGP mRNA expression came into focus in the pancreatic tissues in all notothenioids tested. The use of cdna cloning and sequencing showed that the mRNA all encode secreted AFGPs. An RNA analysis from tissues of a single notothenioid unveiled the anterior ...

Study shows that cells have a natural defense against HIV

... genes in the form of DNA. This DNA copy ?known as cdna ?then travels to the cell nucleus. There, it becom... proviral levels because they were making less HIV cdna or because the HIV cdna was being destroyed before it integrated. To a...
cDNA in Biological Technology

OriGene Announces 37,000 ORF cDNA Clones in a GFP Vector

ROCKVILLE, Md., Oct. 31 /PRNewswire/ -- OriGene Technologies, Inc. announces its flagship products, TrueORF cDNA clones, are available now in a GFP (green fluorescence protein) vector, enabling easy transfection monitoring and protein expression. With this launch, over 25,000 human ORF (open re...

Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

Se-Jin Yoon and Kyung-Ah Lee Infertility Medical Center, CHA General Hospital, Seoul 135-081, Korea Abstract Laser Capture Microdissection (LCM) is an innovative technique which permits the rapid and reliable procurement of pure cell populations from heterogeneous tissue sec...

Inoculation of Viral RNA and cDNA to Potato and Tobacco Plants Using the Helios Gene Gun

Tuija Kekarainen and Jari P. T. Valkonen, Department of Plant Biology, Swedish University of Agricultural Sciences (SLU), P.O. Box 7080, SE-75007 Uppsala, Sweden Introduction Particle bombardment is a physical method widely used for gene transfe...

RNA & cDNA Purification Using RNAClean

RNAClean is a SPRI -based nucleic acid purification system that efficiently isolates RNA or cDNA from in vitro transcription, cDNA or RNA labeling and antisense RNA amplification reactions. As gene expression profiling analysis and the use of ...

RNAClean - RNA & cDNA in vitro Reaction Purification

Agencourts RNAClean kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic RT and IVT reactions such as cDNA and cRNA. The kit is formatted for microarray gene expression analysis...

GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro transcribed cRNA

A crucial step in gene expression analysis is target labeling. To improve the critical steps in the target-labeling process, QIAGEN has developed the GeneChip Sample Cleanup Module specifically for use with Affymetrix GeneChip ...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient Ulrich Genschel Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg ...

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient

Optimizing the Direct Amplification of Missing cDNA 5' Ends Using the Eppendorf Mastercycler gradient Ulrich Genschel Institute of General Botany, University of Hamburg Christian Rohrer, Eppendorf AG, Hamburg ...

Normalizing cDNA libraries using the EppendorfThermomixer

During many experiments in molecular biology, the individual mRNA expression pattern of a cell is examined in order to investigate the reaction of a cell to exogenous influences such as viral infections or medical treatment. To ...

Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes

New GeneConnection discovery microarrays provide more than 4,000 unique human cDNA sequences Stratagene introduces the first in a series of human cDNA microarrays on glass slides. These GeneConnection discovery microarrays II which are based on the ...

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cDNA in Biological Products

TRIMMER-Direct cDNA Normalization Kit from Evrogen JSC

Description: cDNA normalization is essential for hunting and analyzing the rare transcripts using high-throughput approaches, such as EST sequencing projects, microarrays, functional screenings etc. The Evrogen TRIMMER-DIRECT kit is developed for normalization of cDNA enriched with full-length sequences and int...
Company:Evrogen JSC

T7Select1-1 OrientExpress cDNA Cloning System Random Primer from Novagen

Description: ...
Company:Novagen

CyScribe First-Strand cDNA Labeling System - dCTP with CyScribe GFX Purification Kit from GE Healthcare, formerly Amersham Biosciences

Description: CyScribe First-Strand cDNA Labeling System - dCTP with CyScribe GFX Purification Kit, 50 reactions. For the generation and purification of Cy3- and Cy5-labeled cDNA.Optimized reagents are packaged together for complete cDNA labeling and purification.Flexible and optimized protocols can be used for ...
Company:GE Healthcare, formerly Amersham Biosciences

CyScribe First-Strand cDNA Labeling Kit with CyScribe GFX Purification Kit from GE Healthcare, formerly Amersham Biosciences

Description: CyScribe First-Strand cDNA Labeling Kit with CyScribe GFX Purification Kit, 25 reactions. For the generation and purification of Cy3- and Cy5-labeled cDNA.Optimized reagents are packaged together for complete cDNA labeling and purification.Flexible and optimized protocols can be used for performing...
Company:GE Healthcare, formerly Amersham Biosciences

CyScribe First-Strand cDNA Labeling System - dUTP with CyScribe GFX Purification Kit from GE Healthcare, formerly Amersham Biosciences

Description: CyScribe First-Strand cDNA Labeling System - dUTP with CyScribe GFX Purification Kit, 50 reactions. For the generation and purification of Cy3- and Cy5-labeled cDNA.Optimized reagents are packaged together for complete cDNA labeling and purification.Flexible and optimized protocols can be used for ...
Company:GE Healthcare, formerly Amersham Biosciences

Human Skeletal Muscle RACE-Ready cDNA from Ambion

Description: ...
Company:Ambion

Src cDNA Expression Kit (wild type) from Upstate

Description: Src cDNA Expression Kit (wild type)...
Company:Upstate

SuperScript Indirect cDNA Labeling System from Invitrogen

Description: ...
Company:Invitrogen

3' RACE System for Rapid Amplification of cDNA Ends from Invitrogen

Description: ...
Company:Invitrogen

ULS cDNA Synthesis and Labeling Kit (with Biotin) from KREATECH Biotechnology

Description: The ULS cDNA Synthesis and Labeling Kit generates labeled cDNA for use in microarray hybridization experiments. First unmodified cDNA is made via a reverse transcription from mRNA into cDNA using an Oligo(dT) primer. Unlike other methods, there are no unnatural modified nucleotides used in the reve...
Company:KREATECH Biotechnology

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cDNA in Biological Definition

Bioinformatics

...ion analysis The expression of many genes can be determined by measuring mRNA levels with multiple techniques including microarrays , expressed cdna sequence tag ( EST ) sequencing, serial analysis of gene expression (SAGE) tag sequencing, massively parallel signature sequencing (MPSS), or by me...

Bioinformatics

...ion analysis The expression of many genes can be determined by measuring mRNA levels with multiple techniques including microarrays , expressed cdna sequence tag ( EST ) sequencing, serial analysis of gene expression (SAGE) tag sequencing, massively parallel signature sequencing (MPSS), or by me...

Molecular biology

...iction enzyme sites, or to mutate (change) particular bases of DNA. PCR can also be used to determine whether a particular DNA fragment is found in a cdna library . Gel electrophoresis Main article: Gel electrophoresis Gel electrophoresis is one of the principal tools of molecular biology....

Polymerase chain reaction

... gel (note that the band remains in the same location relative to the ladder, it will just appear fatter or brighter). By running samples of amplified cdna from differently treated organisms one can get a general idea of which sample expressed more of the gene of interest. A quantative RT-PCR method has b...

Splicing (genetics)

... exons , 5' cap , leader , trailer and poly-A tail ↓ translation ↓ polypeptide See also : genetics -- cdna ...
cDNA in Biological Dictionary

Vitelline membrane

... > egg characteristics > egg membranes > vitelline membrane ... Human protein-coding gene VMO1. Represented by 37 ESTs from 22 cdna libraries. ... Homo sapiens vitelline membrane outer layer 1 homolog (chicken), mRNA (cDNA ... The dictionary term vitelline membrane was f...

Viral oncogene

...ne for v-myb myeloblastosis viral oncogene homologue (avian), exon 15 ... Human protein-coding gene FOSB. Represented by 916 ESTs from 176 cdna libraries. ... Homo sapiens FBJ murine osteosarcoma viral oncogene -like B variant 8 (FOSB) mRNA, ... ... by tumor viruses that do not themsel...

Untranslated region

...... article "Three prime untranslated region ". Read more ... 3'UTR RNA from Alpha-Tropomyosin Gene Suppresses Tumor Activity. ... Tropomyosin-2 cdna lacking the 3' untranslated region riboregulator induces ... Untranslated , region ,biological ,biology dictionary,biology terminology,biology...

Ubiquitin

...s. ... Proteolysis, the ubiquitin -proteasome system, and renal diseases. ... Mouse protein-coding gene Ubc. Represented by 2568 ESTs from 329 cdna libraries. ... Ubiquitin , becomes conjugated to proteins, marking them for selective ... Ubiquitons and Zomes ( Ubiquitin and Proteasome) ... U...

Tubulin

... Zebrafish protein-coding gene LOC572064. Represented by 26 ESTs from 16 cdna libraries. ... Tubulin beta (LOC572064) SELECTED PROTEIN SIMILARITIES ... Norway rat protein-coding gene Tuba3a. Represented by 47 ESTs from 2 cdna libraries. ... Rattus norvegicus tubulin , alpha 3A, mRNA (cDNA clone MG...

Transport protein

... to this group. ... Organic cation transport protein . IPR011701. Major ... Mouse protein -coding gene Vat1. Represented by 685 ESTs from 170 cdna libraries. ... Mus musculus vesicle amine transport protein 1 homolog (T californica), mRNA ... ... Auxin Transport Proteins ... fluore...

Topoisomerase

... Fruit fly protein-coding gene Top3beta. Represented by 62 ESTs from 17 cdna libraries. ... protein similar to vertebrate topoisomerase (DNA) III be...... Fruit fly protein-coding gene Top1. Represented by 93 ESTs from 19 cdna libraries. ... Topoisomerase I, nuclear enzyme that relieves torsional ...

TATA box

... addition to the TATA Box elements, their extension sequences will also be ... Mouse protein-coding gene Tbp. Represented by 157 ESTs from 81 cdna libraries. ... Mus musculus TATA - box binding protein N-terminal domain splice variant mRNA, ... The human TATA box -Binding Protein (h...

Synaptonemal complex

...l complex . ... synaptonemal complex . synaptosome. Synarchy. synartesis ... Mouse protein-coding gene Sycp3. Represented by 58 ESTs from 25 cdna libraries. ... Mus musculus synaptonemal complex protein 3, mRNA (cDNA clone IMAGE:6774302) ... Human protein-coding gene SYCP1. Represen...

Subcloning

.... The Scientist 1998, 12 ... I promptly borrowed his TOPO TA Cloning kit and set about subcloning those pesky PCR products. ... Subcloning your cdna of interest ... multiple fragments, unsuitable for subcloning directly. ... digestion strategy or PCR subcloning (see instructions below) ... ...
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