Researchers use RNAi libraries to identify regulators of apoptosis and chemoresistance
When it comes to the deadly skin cancer melanoma, studying functional tissue rather than cell lines may better provide insight into the disease's development, according to new research from a Howard Hughes Medical Institute predoctoral fellow at Stanford University School of Medicine. Though multiple genetic alterations are associated with melanoma development, scientists have not been abl...Human Apoptosis Kit 50 tests from BD Biosciences Pharmingen
Description:The BD CBA Human Apoptosis Kit can be used to quantitatively measurecleaved PARP, Bcl-2, and active Caspase-3 protein levels in a single sample. TheKit performance has been optimized for analysis of specific protein in cell lysates....Apo-Direct Apoptosis Detection Kit from eBioscience
Description:The APO-DIRECT Kit is a 2-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; termi...ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit from CHEMICON
Description:The ApopTag Fluorescein Direct In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the direct TUNEL method. The DNA fragments are tagged with a directly labeled fluorescein nucleotide. The kit provides fluorescence staining for 40 samples which are analyzed using flow cytometry....ApopTag Fluorescein In Situ Apoptosis Detection Kit from CHEMICON
Description:The ApopTag Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a Fluorescein reporter molecule. It provides indirect immunofluorescence staining for 40 samples. Results are analyzed using either flow cytometry or fluorescence microscopy....ApopTag Red In Situ Apoptosis Detection Kit from CHEMICON
Description:The ApopTag Red In Situ Apoptosis Detection Kit detects apoptosis in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody with a rhodamine fluorochrome. The kit provides indirect immunofluorescence staining for 40 samples. Results are analyzed using either fluorescence microscopy or flow cytometry (requires a special laser and filters for rhodamine)....RT-PCR Primers for the Study of Apoptosis
Wei-ping Yang Danny Hoang Douglas McKenzie PeterPingerelli Stratagene The apoptotic response is a complex biological process that requires theregulation of a variety of genes that interact with and respond to intracellularand extracellular stimuli. Stratagene is introducing RT-PCR primer<sup...A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
Detection of early apoptotic events has become quiterelevant since many studies have demonstrated thatabnormalities in cell death regulation cause a variety ofdiseases and pathological conditions accompaniedeither by cell accumulation (e.g., cancer) or by cell loss(e...Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
This Application Note describes how the Agilent 2100 bioanalyzer canbe used to analyze apoptotic cell samples. Performance of two assaysfor apoptosis detection is demonstrated and the data are compared tothose obtained with a conventional flow cytometer. Histogram quality...Measuring multiple apoptosis parameters with the Agilent 2100 bioanalyzer
A simplified and fast protocol for the analysis of DNAfragmentation during apoptosis This Application Note describes a simplified and fast protocol for apoptosisDNA fragmentation analysis with the Agilent 2100 bioanalyzerusing the DNA...Citrinin, a fungal toxin, induces apoptosis via mitochondrial pathway
The Mycotoxin citrinin (CTN) is a secondary metabolite produced by several fungal species that belong mainly to the genus Penicillium and Monascus. It is widely considered as a hazard contaminant of foods// and feeds, including corn, wheat, rice, barley and nuts, and is known to be cytotoxic and genotoxic to various mammalian cells. CTN has been suggested as a causative factor in renal disease am...A Combination Of Drugs Used For Anti-Angiogenesis And Apoptosis More Effective
Imantanib is a drug which prevents the growth of new blood vessels, thereby inhibiting the growth of tumour(anti-angiogenesis). //It has been discovered that using Imantanib (Glivec [1]) and a drug that induces cell death (apoptosis) works better. It was found that the combination was more effective in stopping the growth of Ewing's sarcoma in mice than when either drug was given separately....