A fresh look at existing therapies: Researchers explore ways to teach approved drugs new tricks
... Manuel Hidalgo, M.D., Ph.D., suspected that pancreatic cancer − a disease in which EGFR-targeted therapies have proved to be of use, but where amplifications
or mutations in the EGFR gene are rare − is an example of a cancer driven by pathway addiction. The problem, however, is that no one knows what ...
New Mouse Model to Aid in Cancer-causing Genes Hunt
...mphoma, or T-ALL, and the cancer cells showed rampant genome instability resulting in intricate pattern of mutations, chromosomal rearrangements, gene amplifications
and gene deletions similar to those in human solid tumors.
Researchers characterized these mouse tumors by performing high-resolution array-C...
The TripleMaster PCR System for three different amplifications
Christian Rohrer, Eppendorf AG
Standard PCR components (Taq polymerase and reaction buffers) are used for
the general amplification of relatively short DNA fragments ...
ZyGEM Releases Application Note Showing its DNA Extraction Kits Rapidly Produce High Yields of Quality DNA
...evant segment of DNA using PCR reagents supplied in the kits and also with an external amplification system. The prep GEM(TM) Tissue extractions and amplifications
yielded superior results based on both qualitative and quantitative PCR comparisons of the amplified DNA.
"This new application note focusing on ...
GenomiPhi amplification of FFPE tissue DNA for array CGH
...ificity of FFPE DNA
amplification from GenomiPhi amplifications
performing conventional PCR on select human g...s of mean intensities from
DOP-PCR and GenomiPhi amplifications
hybridization characteristics to be simil...om GenomiPhi amplifications. DOP-PCR and GenomiPhi amplifications
showed hybridization characteristics to be similar...
GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH
... produced similar DNA yields, sensitivity and performance. We also showed that BAC DNA generated by the GenomiPhi method detected all 8p12 chromosomal amplifications
that were also detected by the DOP-PCR method in an MDA-MB-134 breast cancer line.
Quantitation of GAD67 Gene Expression in Prefrontal Cortex of Schizophrenia Patients Using the iCycler iQ Detection System and Molecular Beacons, Rev A
... in serial dilutions of cDNA derived from a pooled sample (see above) was
measured (Figure 2A). Figure 2B shows C T ) values of these amplifications
plotted against the logarithm of the relative initial amount of the cDNA.
Theoretically, there should be an inverse linear relat...
Rapid, Reproducible Real-Time Quantitative RT-PCR Using the iCycler iQ Real-Time PCR Detection System and iQ Supermix, Rev A
...d at 20C in a
constant-temperature freezer. Again, only the ACTB-derived transcripts
were amplified (Figure 1B). Traces for late amplifications
(C T > 34) of
the 128 bp primer set represent primer-dimers and not specific product,
as determined by melt-curve and gel analy...
Quantitative Analysis of Protein-DNA Associations in Vivo Using Real-Time PCR, Rev A
...em, and continuous SYBR Green I fluorescence monitoring
was performed according to Bio-Rad protocols. Cycling parameters for all
were 30 sec at 95C, 30 sec at 55C, and 30 sec at 72C,
for 30 cycles. Amplified promoter fragments were subcloned into the T/A
Real-Time Multiplex PCR from Genomic DNA Using the iCycler iQ Detection System
on the amplification efficiencies of both targets. Large differences required
high- (>90%) and equal-efficiency PCR amplifications
of both genes. Amplification
efficiencies of the GAPDH and α-tubulin plasmids were determined prior
to multiplexing and fou...
Temporal Temperature Gradient Electrophoresis: A Powerful Technique to Screen Mutations
3) which contains a 40 base-pair (bp) GC clamp. 9 amplifications
carried out using 100 ng DNA template in 1x PCR buffer (Per....
Mutant and wild-type -thalassemia DNA were isolated from blood. amplifications
were carried out using 100 ng DNA template in 1x PCR buffer, 0.2...
Identification of Disease Causing Mutations in Phenylketonuria by Denaturing Gradient Gel Electrophoresis Using the DCode System
this method by using multiplex PCR* amplifications
combined with Multiplex
evaluated (Michiels et al. 1996). 3 amplifications
out in 20 l reaction mixtur...arable efficiencies,
different exon PCR amplifications
were combined. Moreover, to make sure
GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems
Description:...Designed to degrade PCR products from previous PCR amplifications
without degrading native nucleic acid templates, w...pecifically degrade PCR products from previous PCR amplifications
in which dUTP has been incorporated, without degra...ntaining DNA templates. Products from previous PCR amplifications
are eliminated by excising uracil residues using U...