Laser Capture Microdissection of muscle fiber populations and expression analysis by RT-PCR

...d all MIFs are positive for slow MyHC by immuno-staining. Since mRNA is sensitive to degradation, total time from thawing the slides to dehydration by xylenes was reduced to 32 minutes by combining the histochemical and immunohistochemical reactions and the direct coupling of the antibody to Alexa Fluor 48...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

...s). Slides were immediately placed in 75% ethanol for 30 seconds for fixation, followed by a 15 second distilled water rinse. NOTE: All alcohols and xylenes should be prepared fresh. Steps using 100% EtOH are particularly sensitive, as small amounts of water at this stage may interfere with microdissection...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...atase enzyme completely inhibits the reaction and does not produce any visible staining.

It is important that the alcohols and the xylenes be prepared fresh and not be reused since insufficient dehydra- tion will dramatically affect the ability to perform successful LCM.

...

Xylenes in other areas

Xylene - Wikipedia, the free encyclopedia

The term xylene or xylol refers to a mixture of three aromatic hydrocarbon ... Xylenes are a starting material for the production of other chemicals. ...

Xylenes | Technology Transfer Network Air Toxics Web site | US EPA

Xylenes are released into the atmosphere as fugitive emissions from industrial ... EPA has classified mixed xylenes as a Group D, not classifiable as to human ...

Toxicological Review of Xylenes (PDF)

xylenes, 900 million pounds for o-xylene, 5–8 billion pounds for p-xylene, and 169 million ... Xylenes are distributed throughout the body, but show the ...

ATSDR - ToxFAQs™: Xylenes

Exposure to xylene occurs in the workplace and when you use paint, gasoline, ... Is there a medical test to show whether I've been exposed to xylenes? ...

Xylenes (EHC 190, 1997)

... in Publication Data Xylenes. ... 92% of mixed xylenes is blended into ... At room temperature the xylenes are colourless liquids with an aromatic ...

UW EH&S Xylene distillation and reuse

Hundreds of gallons of xylenes are used each year in pathology laboratories as a ... Xylenes are also used as solvents in some applications because they are less ...