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Xylene in Medical Products

Shandon Consul Coverslipper

Description:...andon Consul is a high throughput automatic glass coverslipper. It provides the best optics for microscopes and image analysis systems. It minimizes xylene exposure with a built-in activated charcoal filter and it integrates with almost all stainers in the market....
Company:ThermoFisher Scientific
Xylene in Biological News

Creation of a new material capable of eliminating pollutants by the hydrocarbon industry

...co Marn . The aim of this study was to prepare and describe a series of new materials monolithic carbon aerogels as adsorbers of benzene, tolene and xylene (BTX). Highly Pollutant The study of the elimination of volatile organic compounds from anthropogenic sources road traffic in cities, solvents,...

Soft-cell approach cuts animal tests

...ma, and pneumonia; fire combustion products, including cyanide, hydrogen sulphide, and ammonia; and, the volatile organic compounds (VOCs) xylene and toluene, found in solvents used by the printing, painting, and petrochemical industries. The in vitro method "opens new possibilities for ...
Xylene in Biological Technology

A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays

...ion was completed, the tissues were immediately processed into paraffin blocks by taking them through a series of graded ethanols, followed by xylene and finally infiltrated with paraffin using an automatic tissue processor. c. Once the tissue was embedded into paraffin block, 7 m ...

Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays

...lace the slides to 100% ethanol for 30 seconds. 13) Place the slides to xylene for 5 minutes. 14) Remove slides from xylene and air dry for 5 minutes. ISOLATION OF SPECIFIC CELL POPULA...

Laser Capture Microdissection (LCM) for the Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

...ylin for ~ 5 sec.
  • Sections are subsequently dehydrated in graded ethanols (95% 2X, 1 min each, 100% 3X, 3 min each ) and cleared in xylene (3X, 2 min each). Allow slides to air dry under a vacuum hood for 30 min (see Special Considerations, Note 2).
  • ...

    Heteroduplex Analysis Using the DCode System

    ...tem. 5 l (200300 ng) of each amplified sample was mixed with 5 l 2x gel loading dye (70% glycerol, 0.05% bromophenol blue, 0.05% xylene cyanol, 2 mM EDTA) and electrophoresed on the DCode system at 100 V for 16 hours at room temperature. After electrophoresis, the...

    DNA Isolation From 50 to 20 mg of Paraffin-Embedded Tissue

    ...10 g) of finely minced tissue in a 1.5 ml capped tube. Add 300 l xylene and incubate 5 min with constant mixing at room temperature. ...scard the xylene. 3. Repeat steps 1 and 2 twice (for a total of 3 xylene washes). 4. Add 300 l of 100% ethanol to the tube and incubate 5 ...

    Preparative Nondenaturing Gel Electrophoresis Used in the Purification of an Esterase Involved in Insecticide Resistance, Rev C

    ..., approximately 60 mM phosphate buffer (pH 6.8). The concentrated sample was mixed with a 5x solution of glycerol and marker dye xylene Cyanole FF to give a final concentration of 10% glycerol and 0.02% dye marker. The sample, approximately 1.6 mg of protein, was ...

    Detection of p53 Gene in Breast Cancer by Denaturing Gradient Gel Electrophoresis and the DCode System

    ...me and the total sample volume made up to 100 l. We added 100 ml 2x gel loading dye (70% glycerol, 0.05% bromophenol blue, 0.05% xylene cyanol, 2 mM EDTA) to these samples, and electrophoresed them on the DCode system at 130 V for 2.5 hours at 56 C. After electrop...

    DGGE Analysis Using the DCode System, Rev B

    ...t delivery system. Mutant sample (200300 ng) was mixed with 5 l of 2x gel loading dye (70% glycerol, 0.05% Bromophenol Blue, 0.05% xylene Cyanole, 2 mM EDTA) and electrophoresed on the DCode system at 130 V for 3 hr at a buffer temperature of 56C. After electrophoresi...

    Optimum FFPE RNA Isolation Kit

    ...essive Amount of Paraffin Used for Embedding Tissues Two times xylene treatment at room temperature should be sufficient for complete ...ous 37-55C treatment can be done for up to 30 minutes. After the xylene deparaffinization, it is crucial that the 100% ethanol is comple...

    Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue

    ...d by the User FFPE tissue xylene JumpStart Taq DNA Polymerase, Product ... microcentrifuge tube. 2. Add 1200 l of xylene and vortex for 30 seconds. 3. Centrifug...e: This protocol can be performed without usage of xylene starting with step number 10. As a resu...
    Xylene in Biological Products

    Gel Loading Dye (Bromophenol Blue, Xylene Cyanol/Glycerol) from Mo Bio Laboratories, Inc.

    Description: Makes gels easy to load and track during electrophoresis. 10X concentrate of Bromophenol Blue, Xylene Cyanol, glycerol solution in TE...
    Company:Mo Bio Laboratories, Inc.

    Xylene Resistant (X/R) Red System, 1000 Tests (SIGNET) from Covance Research Products, Inc

    Description:... Signets xylene Resistant (X/R) Red System consists of concentrated X/R Red Chromogen, X/R Red Activator and X/R Red Buffer. The X/R Red solution reacts with alkaline...
    Company:Covance Research Products, Inc

    Electrophoresis Loading Dye, 6X from Research Organics, Inc.

    Description:... Each bottle contains: Bromophenol Blue 0.25% w/v , xylene Cyanol FF 0.25%, Glycerol solution 30% w/v...
    Company:Research Organics, Inc.

    Gel Loading Dye (Bromophenol Blue/Glycerol) from Mo Bio Laboratories, Inc.

    Description:... Makes gels easy to load and track during electrophoresis. 10X concentrate of Bromophenol Blue, xylene Cyanol, glycerol solution in TE...
    Company:Mo Bio Laboratories, Inc.

    Clarion mounting medium - DAB NBT TNBT Vega Red and Fuchsin from GeneTex

    Description:...s phenomenon is very commonly found in toluene and xylene mounting media due to the fact that the solvent is..., toluene, Hemo-De (limonene), and with all common xylene substitutes of petroleum origin. The absence of xylene makes Clarin an excellent choice where BCIP/NBT ch...
    Company:GeneTex

    Gel Loading Solution (Type I) from Sigma-Aldrich

    Description:... for non-denaturing polyacrylamide and agarose gel electrophoresis of nucleic acids and offers the advantage of two tracking dyes (bromphenol blue and xylene cyanole). Sucrose adds density to facilitate sample loading....
    Company:Sigma-Aldrich

    PAP Pen from ScyTek Laboratories

    Description:...in the target area on the slide. PAP Pen contains a special formulation which is insoluble in alcohol and acetone. It can be removed, if desired, by xylene after the staining procedure is completed....
    Company:ScyTek Laboratories

    One-Step Deparaffinization Solution from Lab Vision

    Description:... of paraffin removal consists of dipping the slides in a series of baths of xylene and graded alcohols. This procedure is time-consuming which takes more than 30 minutes and requires special handling, as xylene is a highly toxic chemical that emits noxious fumes. This product is a xyle...
    Company:Lab Vision

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    Xylene in Biological Dictionary

    Agarose Gel

    ... denaturing agarose gel method for RNA electrophoresis is modified from " ... Add 10X native agarose gel loading buffer (15% ficoll, 0.25% xylene cyanol, 0.25 ... I Agarose gel prepared for DNA analysis - The first lane contains a DNA ladder ... Troubleshooting DNA agarose gel electro...
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