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Xho in Biological Technology

New Competent Cells for Highest Transformation Efficiencies

...ficiency and the potential size bias, two plasmid vectors were used. Stratagenes pcmv-script vector (4.2 kb) was digested with xho I and EcoR I; Stratagenes two-hybrid pAD-GAL4 vector (7.2 kb) was also digested with the same two enzymes. A mouse brain cDNA li...

Generate High-Quality, Directional Plasmid cDNA Libraries

...ligo(dT) linker-primer that contains an xho I restriction site near its 5 end. Fi... ligated to EcoR I adaptors and digested with xho I. Since any internal xho I sites are hemimethylated and protected from ...

Versatile Reporter Vectors for Monitoring Viral Transduction

...umanized, red-shifted A. victoria GFP (EGFP) (data not shown). The coding sequence for hrGFP was inserted between the EcoR I and xho I sites in the pFB vector to produce the pFB-hrGFP vector (Figure 1A). The vector was then tested in retrovirally transduced cel...

A New Lambda Vector for Mammalian Expression

...ust plaque size. We constructed a cDNA library in the EcoR I and xho I sites of the Lambda ZAP-CMV vector using poly(A)+ RNA from hum...picked colonies, and the DNA was digested with either EcoR I and xho I or Not I and Kpn I restriction enzymes. All 12 clones containe...

Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries

...e-stranded cDNA was synthesized using Stratagenes cDNA synthesis kit, and size-selected cDNA was inserted between the EcoR I and xho I sites of the plasmid pFB ( Figure 1A ). The pFB vector has an extended packaging site and uses the viral splice don...

Efficiently Insert Unique Restriction Sites into Plasmid Vectors

...ecognition sites for Bgl II, Pst I, and xho I. ...ely with Stratagenes Bgl II, Pst I, and xho I restriction enzymes. Plasmid DNA was ...y chosen clones. The Bgl II, Pst I, and xho I restriction digestion patterns for se...

An Epitope Tagging Vector for Gene Expression in Mammalian Cells

...mmarized in figure 2. Expression in the pCMV-Tag1 Vector The firefly luciferase gene was cloned into the Bgl II and xho I sites of the pCMV-Tag1 vector such that the luciferase protein was tagged with the FLAG epitope at the N-terminus and the c-my...

Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System

... fluorescent protein (GFP) between the Bgl II and xho I sites into pShuttle-CMV vector. The pShuttle-C...loning the sequence for E. coli LacZ between the xho I and Hind III sites in pShuttle-CMV vector. The...r, and the green fluorescent protein between the xho I and Sal I sites in the pShuttle vector. Ef...
Xho in Biological Products

pLivSelect Cloning Kit A from Bio S&T

Description:.... pLivSelect is also the ideal platform for robot colony picking. This cloning kit can be used for ends generated by Xba I, Nhe I, Hpa I, Hind III and xho I....
Company:Bio S&T

pLivSelect Cloning Kit R-A from Bio S&T

Description:.... pLivSelect is also the ideal platform for robot colony picking. This cloning kit can be used for ends generated by Xba I, Nhe I, Hpa I, Hind III and xho I. Dry-state and stable at room temperature....
Company:Bio S&T

pLivSelect Cloning Kit C from Bio S&T

Description:...is cloning kit is customized as vector DNA is digested using customer-selected group of five restriction enzymes from : Xba I, Nhe I, Hpa I, Hind III, xho I, Aat II, Xma I, Nco I, Mfe I and Sac I....
Company:Bio S&T

pMIR-hU6-Luc, Human U6 Luciferase Control Vector from Mirus Bio Corporation

Description:...anti-sense strand expression cassettes). PCR Expression Cassettes can be cloned using either the Hind III or EcoR I site upstream of the promoter and xho I or BamH I site downstream of the siRNA and termination sequence. The cloning sites used must be unique in the PCR product. Hind III and Xho I are re...
Company:Mirus Bio Corporation

pMIR-mU6, Mouse U6Template (Vector) from Mirus Bio Corporation

Description:...e previous sections can be cloned into the siXpress(TM) Template if the target-specific downstream primer used to generate the PCR products include an xho I or BamH I restriction site. For additional instruction on molecular biology procedures, refer to Sambrook et al....
Company:Mirus Bio Corporation

pMIR-mU6-Luc, Mouse U6 Luciferase Control Vector from Mirus Bio Corporation

Description:...e previous sections can be cloned into the siXpress(TM) Template if the target-specific downstream primer used to generate the PCR products include an xho I or BamH I restriction site. For additional instruction on molecular biology procedures, refer to Sambrook et al....
Company:Mirus Bio Corporation
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