WGA

Inaugural Back of the Envelope Awards fund creative health research

...led whole-genome association (WGA) studies. Feng said he hopes to provide researchers with new algorithms and better software that effectively combine wga study results. Lungu will test the viability of using versatile, tiny carbon "nanotubes" in monitoring devices that measure air quality. Environme...

Fit & Fresh(TM) Launches Filtered Water Bottle

...d about developing a great solution to two problems," said Wesson. Learn more about Fit & Fresh at http://www.fit-fresh.com Learn more about the wga at http://www.wqa.org ...

WGA in Biological Technology

Amplification of genome-representative DNA from limited sources with GenomePlex WGA technology for use in genetic alterations studies

Modern technologies developed in recent years have prompted new and exciting approaches to support biomedical research. The ability to study the entire human genome using limited sources of genomic material to detect genetic disordersmade possible through Sigma-Aldrich Biotechnolo...

International Survey Shows People Over Forty Ignore Risk of Blindness

...d Glaucoma Association and the World Glaucoma Patient Association Both the wga and WGPA exist to better the lives of glaucoma patients around the world. The wga attempts to optimize the quality of glaucoma science and care through commu...

Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

...mplification before attempting WGA. Any wga method to be applied to formalin-fixed DNA must be...gths of nucleic acid stretches, ~1kb or less. Four wga methods have been described and are in common us...mer interactions. There are limits to all wga techniques, and tissue fixation by formaldehyde is...

Development of a CRF1 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System

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Protocol Human recombinant CRF1 receptor membrane preparation was used in conjunction with [ 125 I]Tyr 0 -CRF ligand and wga PS SPA Imaging Beads. Non-specific binding (NSB) was determined in the presence of 200 nM urocortin. The standard assay format was as follows:

Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System

...paration was used in conjunction with [0-methyl- 3 H]Rauwolscine ligand and wga YOx SPA Imaging Beads. Non-specific binding (NSB) was determined in the pre...ise stated. Wells also contained 5 µg of membrane and 250 µg of wga YOx SPA Imaging Beads. Precoupled bead and membrane was added in a 20-&micr...

The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System

...l Human recombinant serotonin 5-HT 2C , edited form, receptor membrane preparation was used in conjunction with [ 3 H]Mesulergine ligand and wga PEI type A PS SPA Imaging Beads. Non-specific binding (NSB) was determined in the presence of 4-µM mianserin. The standard assay format was as f...

GenomiPhi amplification of FFPE tissue DNA for array CGH

...uch method is degenerate oligonucleotide–primed PCR (DOP-PCR).

In contrast to DOP-PCR, GenomiPhi™ DNA Amplification Kit provides wga using a simple isothermal method (4). This method employs the unique biochemical properties of Phi29 DNA polymerase, a highly processive enzyme with e...

Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System

...tion was used in conjunction with [ pyridinyl -5- 3 H]Pyrilamine ligand and wga YOx SPA Imaging Beads. Non-specific binding (NSB) was determined in the pre...ise stated. Wells also contained 5 µg of membrane and 500 µg of wga YOx SPA Imaging Beads. Bead and membrane were premixed immediately prior to...

The development of a calcitonin-gene related peptide (CGRP) receptor-binding assay using the LEADseeker Multimodality Imaging System

... Protocol Human recombinant CGRP receptor membrane preparation produced in CHO-K1 cells was used in conjunction with [ 125 I]CGRP and wga PS LEADseeker SPA Imaging Beads. Nonspecific binding (NSB) was determined in the presence of 500-nM CGRP. The standard assay format was as follows. ...

The development of a melanocortin MC5 receptor binding assay using the LEADseeker Multimodality Imaging System

...ant melanocortin MC 5 receptor membrane preparation produced in CHO-K1 cells was used in conjunction with [ 125 I] NDP-α-MSH (2000 Ci/mmol) and wga PEI Type B PS SPA Imaging Beads. Non-specific binding (NSB) was determined in the presence of 1-µM NDP-α-MSH. The standard assay format wa...

WGA in Biological Products

Glycoprotein Isolation Kit WGA from Pierce Biotechnology, Inc.

Description: Glycosylation is a post-translational modification that plays an important role in biological functions, including immune regulation, inflammation, cell-to-cell adhesion, and cell signaling. Pierce Biotechnology has developed two lectin based Glycoprotein Isolation Kits, Concanavalin A (ConA) and W...
Company:Pierce Biotechnology, Inc.

GenomePlex Single Cell Whole Genome Amplification Kit from Sigma-Aldrich

Description:... fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. wga is achieved by PCR amplification of the library molecules using universal oligonucleotide primers....
Company:Sigma-Aldrich