VersaFluor Cuvette Fluorometer
Description:... The versafluor
cuvette fluorometer is a fast and simple way to quantitate DNA, RNA, and proteins, to detect apoptosis, and to perform reporter enzyme assays with the...
Quantitative Analysis of GFP in Plant Extracts Using the VersaFluor Fluorometer System
Adrian R. Elliott, Peer M. Schenk and Christopher P.L. Grof
CSIRO Tropical Agriculture, Brisbane, Queensland
The Green Fluorescent Protein (GFP) from Aequorea victoria is a useful
reporter gene in higher organisms...
Measuring Intracellular Enhanced Green Fluorescent Protein With the VersaFluor Fluorometer
Contributed by Jeff Wilson, and Andrew Segal, M.D., Genitrix, LLC,
The Green Fluorescent Protein (GFP) derived from Aqueorius victoria has
recently become a popular choice as a reporter gene. GFP can be used...
Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
Nicholas N. Lyssenko and Mark L. Tucker, Soybean and Alfalfa Research Lab,
USDA, ARS, Bldg. 006, BARC-West, Beltsville, MD, USA 20705
In 1987 Jefferson et al. described the use of β-glucuronidase
(GUS) as a marker to ...
Quantitation of RNA for Hybridization Blots Using the VersaFluor Fluorometer
Matthew W. Fields, Section of Microbiology, Cornell University, Ithaca,
New York 14853
Biologists frequently monitor the effect of environmental stimuli on living
organisms by quantitating mRNA levels. The relative abunda...
Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
Wenda Ribeiro and Margaret Saha, Department of Biology, College of William
and Mary, Williamsburg, Virginia 23187
DNA sequence analysis is an integral part of genetic research. Recently,
the use of automated sequen...
The iCycler iQ Detection System for TaqMan Assays, Rev B
... A stock of plasmid pEFGP-Actin encoding β-actin (Clontech) was quantitated
using PicoGreendye (Molecular Probes) binding with the versafluor
(Bio-Rad), then diluted to 10 9 copies/l. A 10-fold dilution series was
prepared from 10 9 to 10 copies/l, and the...
Qualitative and Quantitative Assessment of Plasmid DNA Isolation, Rev A
...re used for plasmid purification
for miniprep and midipreps, respectively. Plasmid concentrations were
quantitated with Bio-Rads versafluor
fluorometer and Hoechst dye 33258
DNA quantitation kit. Plasmid quality was assessed by four criteria: restriction
Thermal Printer, for VersaFluor fluorometer, 120 V from Bio-Rad
Description: The thermal printer, 120 V, is used to record fluorescence data acquired with the VersaFluor
fluorometry systems. The printer includes a cable and power adaptor....
DNA Quantitation Kit (Hoechst) from Bio-Rad
Description:...ed DNA (dsDNA) in solution, even in the presence of RNA or protein. With this kit, DNA is stained with Hoechst 33258 dye, which can be detected with a versafluor
or other fluorometer. The kit quantitates dsDNA in the range of 10 ng per ml to 5 micrograms per ml and contains Hoechst 33258, calf thymus DNA standa...
Thermal Printer Paper, 10 rolls from Bio-Rad
Description:... Thermal printer paper is used with the thermal printer for the versafluor
fluorometry systems. Supplied as 10 rolls....