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Uracil in Medical Technology

Alchemia Reports Preliminary Positive Efficacy Data From HyCAMP Phase II Trial

...ne 2006. In the randomised trial, 80 patients with metastatic colorectal cancer who had previously failed treatment with the anti-cancer drug 5-fluoro uracil were eligible to receive up to eight cycles of chemotherapy in the form of irinotecan or HyCAMP(TM) intravenously. The primary endpoint of the trial w...
Uracil in Biological News

New tool isolates RNA within specific cells

...on the UO project as a postdoctoral fellowship funded by the National Institutes of Health and HHMI. Cleary's group built its tool with the enzyme uracil phosphoribosyltransferase (UPRT), a nucleotide salvage enzyme that prepares nucleotides for incorporation into newly synthesized RNA. By altering the ...

SNPs affect folate metabolism in study of Puerto-Rican adults

...s for altered folate metabolism and the content of uracil in blood DNA. In a study published in October's A...olve these SNPs that affected the concentration of uracil in DNA. uracil accumulation has the potential to cause DNA breaka...

News tips from the Journal of Neuroscience

...nclude increased levels of homocysteine, increased uracil incorporation into DNA, and increased DNA strand breaks due to uracil excision by uracil-DNA glycosylase (UNG). To inves...genotype, suggesting that an increased presence of uracil in DNA contributes to some but not all effects of ...

Scientists confirm that parts of earliest genetic material may have come from the stars

...ence Letters , provides evidence that life's raw materials came from sources beyond the Earth. The materials they have found include the molecules uracil and xanthine, which are precursors to the molecules that make up DNA and RNA, and are known as nucleobases. The team discovered the molecules in r...

Finding that 1-in-a-billion that could lead to disease

...threatening genetic damage actually recognize DNA errors. Appearing online next week in Nature, the Hopkins team describes how the UDG enzyme (for uracil DNA glycosylase) scrutinizes the shape of DNA building blocks by holding onto them and testing their fit into a specially sized pocket. The UDG pocket...
Uracil in Biological Technology

Glossary for The BioPharm Guide to Fermentation and Cell Culture

...to thymine (T). In RNA molecules, adenine joins to uracil (U). . batch culture Large-scal...tosine (C), guanine (G), thymine (T), or uracil (U). nucleus The largest organelle, a s...d on ribose, and with the base uracil (U) in place of thymine (T). Various forms of RNA ...

Transformation of Filamentous Fungi by Microprojectile Bombardment

...Kansas City, Kansas, and maintained on solid YG medium [0.5% yeast extract, 2% glucose, 2% agar, pH 6.3] supplemented with 0.12% uracil and 0.12% uridine. Plates were incubated for 68 days at 37 C at which time conidia were harvested in 0.9% sterile saline. The re...

Transformation of Nematodes With the Helios Gene Gun

...ivated on NGM agar plates (Brenner, 1974). Wild-type worms (strain N2 var. Bristol) are fed on Escherichia coli OP-50 (exogenous uracil dependent mutant) seeded on those plates. The surface of a densely populated NGM agar plate is subsequently rinsed with tap water. A drop ...

Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

... al., 1992b). Yeast The effects of microparticle size, helium pressure, and target distance on transformation of the uracil auxotroph S. cerevisiae st 948 with the uracil-containing plasmid YEp352 are shown in Table 3. The initial experiment (Table 3, ...

Quantitation of Lymphangiogenesis Using the iCycler iQ Real-Time PCR Detection System and Scorpions Detection System, Rev A

...tection system, the plasmid standards and breast cancer cDNA were simultaneously assayed in duplicate 15 l reactions as follows: uracil DNA glycosylase supermix (7.5 l), forward primer/probe (0.3 l), reverse primer (0.3 l), plasmid or specimen cDNA (1 l), water (5...

PCR Polymerases Application Profiles

... Defines whether an enzyme system can be used with dUTP instead of dTTP to apply procedures for PCR carry-over prevention using uracil DNA glycosylase (UNG). ...

Uracil-DNA Glycosylase

...5 units Description Uracil-DNA Glycosylase (UNG) (from E. coli) catalyzes the removal of uracil from single- and double-stranded DNA that has been synthesized in the presence of dUTP. The apyriminic sites formed by UNG are susce...

Epitope-Tagging Vectors for Functional Analysis in Yeast

...gene encodes orotidine-5 -phosphate decarboxylase, which is required for uracil biosynthesis. Plasmids bearing URA3 can be counter-selected by plating th...in amino acid biosynthesis, the URA3 gene product is involved in uracil biosynthesis. Marker gene activity has been shown to influence v...
Uracil in Biological Products

GeneAmp PCR Carry-over Prevention Kit from Applied Biosystems

Description:...ture, and pretreating all subsequent PCR mixtures with the enzyme, AmpErase uracil N-glycosylase (UNG) prior to PCR amplification.1 Products from PCR amplific...lates. Products from previous PCR amplifications are eliminated by excising uracil residues using UNG, and degrading the resulting abasic polynucleotide with ...
Company:Applied Biosystems
Uracil in Biological Definition

Anticodon

...s inosine which allows that base to pair with cytosine , adenine , and uracil on the codon. The first base on the anticodon can also have a uracil which can base pair with guanine as well as adenine. ...

Genetic code

...midine bases cytosine ( C ) and thymine ( T ) are smaller and only consist of one aromatic ring. In RNA however, thymine ( T ) is substituted by uracil ( U ) and the deoxyribose is substituted by ribose . Overall, there are 4 3 = 64 different codon combinations. For example, the RNA sequence UUU...

Genetic code

...midine bases cytosine ( C ) and thymine ( T ) are smaller and only consist of one aromatic ring. In RNA however, thymine ( T ) is substituted by uracil ( U ) and the deoxyribose is substituted by ribose . Overall, there are 4 3 = 64 different codon combinations. For example, the RNA sequence UUU...

Growth curve

...nsitive to the toxic effects that conventional anticancer drugs have on the cancer cells . Many conventional anticancer drugs (for example, 5-Fluoro uracil ) interfere with DNA replication and can cause the death of cells that attempt to replicate their DNA and divide. A rapidly growing tumor will have ...

Nucleoside

... Deoxyadenosine dA Guanine Guanosine G Deoxyguanosine dG Thymine Thymidine T Deoxythymidine dT uracil Uridine U Deoxyuridine dU Cytosine Cytidine C Deoxycytidine dC Nucleosides are glycosylamines made by a...

Nucleotide

... (r) or deoxyribonucleotide (d). The second letter indicates the nucleobase: G: Guanine A: Adenine T: Thymine C: Cytosine U: uracil not present in DNA, but takes the place of Thymine in RNA The third and fourth letters indicate the length of the attached phosphate chain (Mon...

Purine

...h their complementary pyrimidines thymine and cytosine . purine pyrimidine A T G C In RNA , the complement of A is uracil instead of thymine: purine pyrimidine A U G C Adenine Guanine These hydrogen bonding modes are fo...

Pyrimidine

...o nitrogen atoms at positions 1 and 3 of the six-membered ring. Three nucleobases found in nucleic acids , namely cytosine , thymine , and uracil , are pyrimidine derivatives. In DNA and RNA , these bases form hydrogen bonds with their complementary purines . purine pyrimidine A ...

RNA

... to each pentose ring; as well as by the use of uracil , instead of thymine . One of the main functions ...rent bases: adenine , guanine , cytosine , and uracil . The first three are the same as those found in DNA, but uracil replaces thymine as the base complementary to ad...

Wobble base pair

...t modified base is inosine which can basepair with three bases including uracil , adenine , and cytosine . Another critical base pair is the G-U base pair which allows uracil to base pair with two bases: guanine and adenine . Links ...
Uracil in Biological Dictionary

Uracil

Uracil is a common and naturally occurring pyrimidine derivative. Originally discovered in 1900, it was isolated by hydrolysis of yeast nuclein that was found in bovine thymus and spleen, herring sperm, and wheat germ. It is... uracil n. ( Abbr. U ) A pyrimidine base, C 4 H 4 N 2 O 2...

Untranslated RNA

...RNA molecules that are processed into short (17 ... ... acid ( RNA ) is a ... RNA . 2.1 Untranslated Regions. 2.2 Anti-sense mRNA. 3 RNA ... uracil is appropriate for RNA , where quantity is important but ... RNA is created in the transcription; after splicing and polyadenylation it is ...

Thymine

...sts, thymine may be derived by methylation of uracil at the 5th carbon. In RNA,... A thymine dime...e five main ... Nucleobases: Adenine - Thymine - uracil - Guanine - Cytosine - Purine - Pyrimidine ... ...leic acid DNA. In RNA thymine is replaced with uracil in most ... Nucleobases: Adenine - Thymine -...

RNA

... Information about RNA in the free online English dictionary and encyclopedia. ... RNA also differs from DNA in having the pyrimidine base uracil instead of ... RNA's sister Web sites. ReligionWriters.com. ReligionLink.com. ReligionHeadlines.org ... Why join RNA ? How to join or renew. E-...

Ribonucleotides

...eotide in which a purine or pyrimidine base is linked to a ribose molecule. The base may be adenine (A), guanine (G), cytosine (C), or uracil (U). Note that thymine (T), which is found in deoxyribonucleotides, is not found as a ribonucleotide in living beings. Ribonucleotides have one, ...

Pyrimidine

...degraded or salvaged for ... Pyrimidine is a heterocyclic aromatic organic compound similar to benzene and ... Nucleobases: Adenine - Thymine - uracil - Guanine - Cytosine - Purine - Pyrimidine ... pyrimidines , purine pyrimidine ... pyrimidine ... The simplest member, pyrimidine itself...

Purine

...uch as anchovies, broth, and dried ... Purine is a heterocyclic, aromatic, organic compound, consisting of a ... Nucleobases: Adenine - Thymine - uracil - Guanine - Cytosine - Purine - Pyrimidine ... Online Medical Dictionary and glossary with medical definitions ... The purine bases are guan...

Guanine

...atson-Crick base pairing forms three ... Nucleobases: Adenine - Thymine - uracil - Guanine - Cytosine - Purine - Pyrimidine ... Compare Cheap Prices fo... a ... The main ones are cytosine, guanine , adenine , thymine and uracil , abbreviated ... Guanosine is a nucleoside comprising guanine attache...

Deoxyribonucleotide

... polymer composed of four deoxyribonucleotide units with a nitrogenous base of adenine, guanine, thymine or cytosine. Under certain conditions, uracil can ... Processes and reagent kits employing terminal deoxynucleotide transferase are provided for terminally chemically ... labeled deoxyribonuc...

Deamination

...rolytic deamination of cytosines in DNA creates uracil and, if unrepaired, ... It decreases the rate of cytosine deamination to uracil by 7-fold. ... Enzymes mediating tRNA adenosi...s shown in the above figure, it may be mutated to uracil by deamination . ... ... of our nitrogenous w...
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