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Unmethylated in Medical News

Stem cells give clues to understanding cancer and make breakthrough in childhood leukaemia

... that in some leukaemias there is a gene involved in differentiation that is methylated, Dr Esteller said. In cultured cells we see that if we put the unmethylated gene back into the cell, we stop the growth of the cells in culture, and also in mouse models. This gene is acting as a tumour suppressor. The hope...

Liver cancer marker could yield blood test for early detection

... PCR, which combines real-time PCR, a technique that enables researchers to simultaneously detect and amplify a given gene, with an enzyme that breaks unmethylated DNA apart. With this new technique, Chans team was able to separate out the altered methylated DNA, thus developing a more sensitive technique for de...
Unmethylated in Biological News

The evolutionary foundation of genomic imprinting in lower vertebrates

...ntal differentially methylated region that was hypermethylated in sperm but unmethylated in eggs. The authors also indicated that, unlike mammalian imprinting gene DMR, the unmethylated DMR of maternal allele could not resist methylation reprogramming an...

NYU and MSKCC research provides model for understanding chemically induced cancer initiation

...in which the carcinogenic residue is sandwiched between adjacent base pairs in the double helix. "Such conformational differences in methylated and unmethylated DNA sequences may be significant because of potential alterations in the cellular processing of these lesions by DNA transcription, replication, and r...
Unmethylated in Biological Products

RNA Cap Analog, Unmethylated from EPICENTRE Biotechnologies

Description: A modified nucleotide structure called a ?cap? is present on the 5?-end of most naturally-occurring eukaryotic mRNAs and many viral RNAs. Substitution of Cap Analog for a portion of the GTP present in an in vitro transcription reaction results in the synthesis of transcripts with a cap on the 5'-en...
Company:EPICENTRE Biotechnologies

Bacterial Strain BMH 71-18 mutS, Glycerol Stock (noncompetent) from Promega

Description:... BMH 71-18 mutS and ES1301 mutS are mismatch repair minus strains of E. coli. Use of these strains prevents repair of the newly synthesized unmethylated strand (1), leading to high mutation efficiencies and making them helpful in such systems as the GeneEditor and Altered Sites II Mutagenesis Systems. ...
Company:Promega

Bacterial Strain ES1301 mutS, Glycerol Stock (noncompetent) from Promega

Description:... BMH 71-18 mutS and ES1301 mutS are mismatch repair minus strains of E. coli. Use of these strains prevents repair of the newly synthesized unmethylated strand (1), leading to high mutation efficiencies and making them helpful in such systems as the GeneEditor and Altered Sites II Mutagenesis Systems. ...
Company:Promega

CpG WIZ® DAP-KINASE AMPLIFICATION KIT from CHEMICON

Description:...ic primers designed to distinguish methylated from unmethylated DNA. The CpGenome™ DNA Modification Kit (S78...he sequence differences between the methylated and unmethylated DNA. The primer sets in the kit are engineered to ... sequence differences. U Primer Set will anneal to unmethylated DNA that has undergone a chemical modification. M ...
Company:CHEMICON

CpGenome™ Fast DNA Modification Kit from CHEMICON

Description:...on on a DNA sample. In the bisulfite reaction, all unmethylated cytosines are deaminated and sulfonated, convertin...specifically amplify either a bisulfite-sensitive, unmethylated strand or a bisulfite-resistant, methylated strand...abeled as 'U') designed to be complementary to the unmethylated bisulfite converted DNA sequence. No product will ...
Company:CHEMICON

EZ-96 DNA Methylation Kit - Deep Well from Zymo Research

Description:...ditions. The kit is designed to reduce template degradation, minimize DNA loss during treatment and clean-up, and result in the complete conversion of unmethylated cytosines. The recovered, treated DNA template is ideal for Methylation Specific PCR (MSP) followed by methylation analysis using restriction endonucl...
Company:Zymo Research

EZ DNA Methylation Kit from Zymo Research

Description:...ditions. The kit is designed to reduce template degradation, minimize DNA loss during treatment and clean-up, and result in the complete conversion of unmethylated cytosines. The recovered, treated DNA template is ideal for Methylation Specific PCR (MSP) followed by methylation analysis using restriction endonucl...
Company:Zymo Research

EZ-96 DNA Methylation Kit from Zymo Research

Description:...ditions. The kit is designed to reduce template degradation, minimize DNA loss during treatment and clean-up, and result in the complete conversion of unmethylated cytosines. The recovered, treated DNA template is ideal for Methylation Specific PCR (MSP) followed by methylation analysis using restriction endonucl...
Company:Zymo Research

EpiTect Bisulfite Kit from QIAGEN

Description:... The EpiTect Bisulfite Kit enables complete conversion of unmethylated cytosines to uracils in less than 6 hours. The highly sensitive method utilizes an innovative protection against DNA degradation and a spin-column-bas...
Company:QIAGEN
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