Best New Product Awards Winners Named at Molecular Medicine Tri-Conference

... Seahorse Biosciences Discovery Informatics Labmatrix by BioFortis Best New Product Award Winners ClearPoint Peptides By spiking protein tryptic digests with internal heavy isotope labeled peptide standards, Mass Spectrometry becomes an absolute quantitation (AQUA) method. With a choice of hea...

Tryptic in Biological Technology

Four Independent Research Groups Present Data Generated by Pressure Cycling Technology (PCT) at the ASMS Conference on Mass Spectrometry and Allied Topics in Denver, CO

... discussed the effect of high pressure (up to 35,000 psi) -- with pressure cycling technology (PCT) and with an on-line high pressure system -- on the tryptic digestion of proteins. They reported that complete digestion of proteins could be achieved in just 60 seconds. The researchers further reported that h...

Americas Third Coast nurtures biotechnology

... plates. The plated fractions can then be analyzed repeatedly by MALDI-TOF MS. The MALDILC Systems HPLC-coupled fraction collection simplifies complex tryptic digests, which provides more readable MS spectra. The MALDILC System automates what has been a historically manual procedure. Lucigen ( www.lucig...

Testing Compressed Air Lines for Microbiological Contamination

... compressed air system. The agar used can be a broad spectrum agar such as Soybean Casein Digest Agar (SCDA), commonly referred to as tryptic Soy Agar (TSA) which will allow the growth of most non-fastidious organisms. A selective agar may be used if you are trying to isolate a cer...

Fundamentals of Fermentation: Techniques For Benchtop Fermentors

... used in a fermentation vessel. Shake flask media is generally of a much simpler composition. LB Broth (Difco catalog number 0446-17-3 ) and tryptic Soy Broth (Difco catalog number 0370-07-5) are standard shake flask media. An example of a more complex media used in a recombinant...

Glossary for The BioPharm Guide to Fermentation and Cell Culture

... a polypeptide (protein) chain. trypsin, tryptic digestion Trypsin allows the growth of cells as i... cells from their attached substratum. tryptic fragment analysis Quantitating the resultant fragments caused by tryptic digestion. turbidostat A variation on a...

Protein Depletion for Plasma and Serum Proteomic Analysis

... peptide fragments. With or without another separation step, the tryptic peptides are analyzed by mass spectrometry to establish their id... are proteolytically digested into peptides. The peptides in the tryptic digest are then separated by multidimensional separation techniq...

Testing Compressed Air Lines for Microbiological Contamination

...in your compressed air system. The agar used can be a broad spectrum agar such as Soybean Casein Digest Agar (SCDA), commonly referred to as tryptic Soy Agar (TSA) which will allow the growth of most non-fastidious organisms. A selective agar may be used if you are trying to isolate a certain type ...

Complex Peptide Mixture Analysis on the Finnigan LTQ Linear Ion Trap Mass Spectrometer

... faster decisions on proteomics research samples. Acknowledgements We would like to thank Terry Zhang for providing the plasma tryptic digest sample used in this report, and Rohan Thakur, Gargi Choudhary, Julian Phillips, and Jae Schwartz for their technical assistance. ...

Fully Automated Nano-Electrospray Coupled with a Finnigan LCQ Deca XP Plus for Sub-fmol Proteomic Analysis

... charged precursor ions. Results and Discussion The tryptic digest of horse myoglobin was analyzed by nanospray liquid chromatography a...low rates of 100 nL/min. Two different amounts (250 fmol and 500 fmol) of a tryptic myoglobin digest were analyzed three time each and the elution time of each...

Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma

...d MIDAS workflow on the MIDAS TRAQ system to detect and quantitate selected tryptic peptides, where each peptide represents a specific candidate protein biomar...he MIDAS workflow software designer. From the protein sequence, theoretical tryptic peptides are determined and the fragmentation pattern of the peptide by MS/...

Tryptic in Biological Products

ProteoExtract Protein Precipitation Kit from Calbiochem

Description:...y be re-suspended in the buffer of choice for a wide range of proteomics downstream applications such as isoelectric focusing (IEF), 2DGE, 1DGE or for tryptic digestion prior to mass spectrometry and peptide separation schemes. • The ProteoExtract™ Protein Precipitation Kit can be used with virtua...
Company:Calbiochem

ProteoSpin Micro Detergent Clean Up Kit from Norgen Biotek Corporation

Description:...g SDS, Triton X-100, CHAPS, NP-40, and Tween 20. Effective detergent removal - Removes more than 95% of detergents for many protein samples, allowing tryptic digestion. High recovery - Recoveries of up to 95% for many proteins. Fast processing time - In contrast to longer dialysis procedures, the easy-to-...
Company:Norgen Biotek Corporation

Protein Identification Service via MALDI MS/MS from Protagen AG

Description:... This protein identification Service includes 1. Scanning of gel(s) 2. Excision/picking of protein spots 3. High-sensitive tryptic in-gel digestion and peptide extraction 4. Preparation of samples for MALDI-MS 5. Acquisition of PMF (Peptide Mass Fingerprint) spectra via MALDI-MS (...
Company:Protagen AG