Trypsin in Biological News
Reversible 3-D cell culture gel invented
...quired amount from the liquefied gel.
Unlike conventional cell culture, trypsin
is not required to detach the cultured cells from the solid media. As trypsin
is an enzyme that is known to damage cells, especially in stem cell
Cellular self-eating promotes pancreatitis
... cells surges. Pancreatitis occurs when the enzyme trypsin
dissolves cells from within. Normally, pancreatic cells fashion and discharge an inactive form of trypsin
called trypsinogen, which remains inert until it r...he small intestine. But if trypsinogen converts to trypsin
before its release, it can damage or kill a pancre...
Trypsin in Biological Technology
Pressure BioSciences, Inc. Announces the Market Release of its PCT-Enhanced Trypsin Digestion Application for Proteomics
SOUTH EASTON, Mass., Aug. 6 /PRNewswire-FirstCall/ -- Pressure
BioSciences, Inc. (Nasdaq: PBIO ) ("PBI" or "the Company") today announced
the market release of its pressure cycling technology ("PCT")-enhanced
trypsin digestion application for proteomics, a major new addition to its
Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update
...y of several new and important
PCT-based products and applications, including the use of PCT to
significantly enhance (hours to minutes) the important trypsin
phase required prior to protein identification by mass spectrometry
Richard T. Schumacher, Founder, President, and CEO of Pressure
Four Independent Research Groups Present Data Generated by Pressure Cycling Technology (PCT) at the ASMS Conference on Mass Spectrometry and Allied Topics in Denver, CO
..., as compared to current
Similar to the trypsin
digestion study carried out by scientists at
PNNL,...PCT to "dramatically
reduce" the time required for trypsin
digestion of proteins and to
"simplify the procedu...mpared with current sample preparation
methods for trypsin
digestion of proteins; the importance of
Glossary for The BioPharm Guide to Fermentation and Cell Culture
...ids in a polypeptide (protein) chain.
trypsin, tryptic digestion trypsin
allows the growth of cells as indepen
dent microorganisms distinct from t...epeated culture through cycles of growth, trypsinization, and
is also used to remove anchorage-dependent cells from
their attached subs...
Protein Depletion for Plasma and Serum Proteomic Analysis
... and the individual protein spots
or fractions are digested with trypsin
to yield peptide fragments. With
or without another separation s...fferent
multi-dimensional separation techniques are subjected to trypsin
and analyzed by LC-mass spectrometry. Multi-dimensiona...
Fully Automated Nano-Electrospray Coupled with a Finnigan LCQ Deca XP Plus for Sub-fmol Proteomic Analysis
... reduced and alkylated proteins were digested by adding 20 μL modified trypsin
(0.5 mg/mL, Promega, Madison, WI, USA). The mixture was incubated for 6 hours at 37C, then an additional 20 μL trypsin
(0.5 mg/mL) was added and incubation was continued for 16 hours at 37C.
HEK293 Calcium Flux Assay
...h hand to displace cells and verify cell lifting under microscope. Stop the trypsin
reaction by adding 10 mL of cell growth media and triturate cells. Seed a T...h hand to displace cells and verify cell lifting under microscope. Stop the trypsin
reaction by adding 5 mL of cell growth media. Triturate cells and add to a ...
Calcium Flux Assays using the LabChip 3000
...DTA. The EDTA was aspirated and 2 mL of Trypsin-EDTA solution was added. After ~ 5 minutes, it was confirmed that the cells were suspended and 1 mL of trypsin
inhibitor solution was added to stop the reaction.
9 mL of cell preparation buffer (See Section IV) was added...
Fluorescence assay for determination of protease activity
...y quenched BODIPY FL-casein substrate releasing fluorescent peptides. The trypsin
used as standard was diluted in 10 mmol/l Tris-HCl buffer, pH 7.8 of which ...20-041 VICTOR 2 V with stacker
OTHER PRODUCTS NEEDED
enzyme from bovine pancreas, activity 10700 units/mg (Sigma T-8253)
Affinity Depletion Cartridges for Removal of Human Serum Albumin and Immunoglobulins from Human Serum
...t molecular weight of human Albumin using Voyager DE STR Biospectrometry Workstation (ABI) from in-gel digested peptides using 10ug/mL modified bovine trypsin
in 25mM ammonium bicarbonate.
Anti-HSA and anti-human IgG ELISA kits (Bethyl Laboratory) were run according to the manufacture protocol. The...
Trypsin in Biological Products
Porcine TRYPSIN Native from AbD Serotec
Description: NATIVE, ENZYME PORCINE TRYPSIN...
Pig Trypsin from BIOPUR
(Pig Pancreas) (TPCK Treated, Highly Purified)...
Trypsin-EDTA (0.05% Trypsin EDTAo4Na)(1X) liquid from Invitrogen
Description: Porcine parvovirus and mycoplasma tested. Contains 0.5 g/L of trypsin
(1:250) and 0.2 g/L of EDTA4Na in Hanks' Balanced Salt Solution without CaCl2 MgCl2 6H2O and MgSO4 7H2O. Contains phenol red....
Rabbit Protein Phosphatase 1 (PP1) from Upstate
Description:...gCl2, 1mM EGTA, 10 Properites: For selective in vitro dephosphorylation of proteins Purification: DEAE chromatography, divalent cation activation plus trypsin
digestion, and gel filtration chromatography Quality Assurance: routinely evaluated by dephosphorylation of proteins
SilverQuest Silver Staining Kit from Invitrogen
Description:...raditional silver stains are eliminated with the SilverQuest kit because the reagents used do not modify protein side chains and do not interfere with trypsin
Protein Purification Plate Centrifuge compatible from ABgene
Description:... Quick and simple protocol less than 40 minutes Purifies full-length proteins from SDS-PAGE gels MALDI-TOF compatible Protocol can be followed by trypsin
digest if required Plates are centrifuge-compatible
Applications N-terminus sequencing Immuno-labelling Protein quantification MALDI-TO...
2-D SDS-PAGE Standards, 500 microliter from Bio-Rad
Description:... These 2-D standards consist of seven reduced, denatured proteins (equine myoglobin, soybean trypsin
inhibitor, bovine carbonic anhydrase, rabbit muscle GAPDH, bovine muscle actin, BSA, and hen egg white conalbumin) with pIs ranging from 4.5 to 8.5 an...
Trypsin in Biological Definition
...ition in animals relies on digestive enzymes such as salivary amylase , trypsin
and chymotrypsin . Their primary role is for the digestion of food and ma...ismutase : transforms superoxide into oxygen and hydrogen peroxide
: digests proteins
Tyrosine kinases : those enzymes which transfer phosp...
Conversion of predecessor-proteins (proenzymes , zymogens , prehormones ) into their final structures.
Examples of proteases are trypsin
and chymotrypsin .
Trypsin in Biological Dictionary
...rs many protein analysis products. These include in vitro expression systems, protein purification and labeling systems, expression vectors, trypsin
and ... In the PISA ( protein in situ array ) method, tagged functional proteins are made ... This enables a protein array to be made in a ...
...ining condensed chromosomes. The metaphase chromosomes are treated with trypsin
and stained with Giemsa. Dark bands that take up the stain are strongly A,T...l content from favorite medical titles, including Harrison's Online. ... trypsin
G-banding stain information including symptoms, causes, diseases, symptom...