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Trypsin in Medical News

Arterial infusion using gabexate mesilate: Is it effective therapy for severe acute pancreatitis?

... The level of protease inhibitor in pancreatic tissues after CRAI using nafamostat was 5-fold higher than that delivered by intravenous injection, and trypsin activities in pancreatic tissues are significantly suppressed by CRAI. On the other hand, the level of protease inhibitor in pancreatic tissues after ...

Pressured proteins: A little pressure in proteomics squeezes 4-hour step into a minute

... The researchers incubated albumin with the enzyme trypsin at several different pressures for one minute each, then counted how many pieces into which trypsin cut albumin. At 10,000 pounds-per-square-inch up to 35,000 psi, trypsin appeared to maximally cleave albumin. For these te...

Monoclonal antibody Hb3: A marker for colon cancer progression or as a therapeutic target?

...en Ca-Hb3 which is recognized by colorectal carcinoma monoclonal antibody Hb3, Ca-Hb3 was isolated by SDS-PAGE followed by digestion with trypsin. The trypsin peptides were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Applying bioinformatics to analyze the proteins which were identi...

The most important candidate genes for pancreatic stone formation

...he gene variants could predispose the reg1 protein to increased cleavage by trypsin and form fibrils that may precipitate and obstruct the duct by forming prot... the established susceptibility genes for TCP, such as pancreatic secretory trypsin inhibitor and cathepsin B (encoded by SPINK1 and CTSB respectively). On...
Trypsin in Biological News

Reversible 3-D cell culture gel invented

...quired amount from the liquefied gel. Unlike conventional cell culture, trypsin is not required to detach the cultured cells from the solid media. As trypsin is an enzyme that is known to damage cells, especially in stem cell culture...

Cellular self-eating promotes pancreatitis

... cells surges. Pancreatitis occurs when the enzyme trypsin dissolves cells from within. Normally, pancreatic cells fashion and discharge an inactive form of trypsin called trypsinogen, which remains inert until it r...he small intestine. But if trypsinogen converts to trypsin before its release, it can damage or kill a pancre...
Trypsin in Biological Technology

Pressure BioSciences, Inc. Announces the Market Release of its PCT-Enhanced Trypsin Digestion Application for Proteomics

SOUTH EASTON, Mass., Aug. 6 /PRNewswire-FirstCall/ -- Pressure BioSciences, Inc. (Nasdaq: PBIO ) ("PBI" or "the Company") today announced the market release of its pressure cycling technology ("PCT")-enhanced trypsin digestion application for proteomics, a major new addition to its PCT family...

Pressure BioSciences, Inc. Reports Second Quarter 2008 Financial Results and Provides Business Update

...y of several new and important PCT-based products and applications, including the use of PCT to significantly enhance (hours to minutes) the important trypsin digestion phase required prior to protein identification by mass spectrometry Richard T. Schumacher, Founder, President, and CEO of Pressure BioScie...

Four Independent Research Groups Present Data Generated by Pressure Cycling Technology (PCT) at the ASMS Conference on Mass Spectrometry and Allied Topics in Denver, CO

..., as compared to current methods. Similar to the trypsin digestion study carried out by scientists at PNNL,...PCT to "dramatically reduce" the time required for trypsin digestion of proteins and to "simplify the procedu...mpared with current sample preparation methods for trypsin digestion of proteins; the importance of ProteoSol...

Glossary for The BioPharm Guide to Fermentation and Cell Culture

...ids in a polypeptide (protein) chain. trypsin, tryptic digestion trypsin allows the growth of cells as indepen dent microorganisms distinct from t...epeated culture through cycles of growth, trypsinization, and subculture. trypsin is also used to remove anchorage-dependent cells from their attached subs...

Protein Depletion for Plasma and Serum Proteomic Analysis

... and the individual protein spots or fractions are digested with trypsin to yield peptide fragments. With or without another separation s...fferent multi-dimensional separation techniques are subjected to trypsin digestion and analyzed by LC-mass spectrometry. Multi-dimensiona...

Fully Automated Nano-Electrospray Coupled with a Finnigan LCQ Deca XP Plus for Sub-fmol Proteomic Analysis

... reduced and alkylated proteins were digested by adding 20 μL modified trypsin (0.5 mg/mL, Promega, Madison, WI, USA). The mixture was incubated for 6 hours at 37C, then an additional 20 μL trypsin (0.5 mg/mL) was added and incubation was continued for 16 hours at 37C. ...

HEK293 Calcium Flux Assay

...h hand to displace cells and verify cell lifting under microscope. Stop the trypsin reaction by adding 10 mL of cell growth media and triturate cells. Seed a T...h hand to displace cells and verify cell lifting under microscope. Stop the trypsin reaction by adding 5 mL of cell growth media. Triturate cells and add to a ...

Calcium Flux Assays using the LabChip 3000

...DTA. The EDTA was aspirated and 2 mL of Trypsin-EDTA solution was added. After ~ 5 minutes, it was confirmed that the cells were suspended and 1 mL of trypsin inhibitor solution was added to stop the reaction. Suspending Cells 9 mL of cell preparation buffer (See Section IV) was added...

Fluorescence assay for determination of protease activity

...y quenched BODIPY FL-casein substrate releasing fluorescent peptides. The trypsin used as standard was diluted in 10 mmol/l Tris-HCl buffer, pH 7.8 of which ...20-041 VICTOR 2 V with stacker OTHER PRODUCTS NEEDED trypsin enzyme from bovine pancreas, activity 10700 units/mg (Sigma T-8253) ...

Affinity Depletion Cartridges for Removal of Human Serum Albumin and Immunoglobulins from Human Serum

...t molecular weight of human Albumin using Voyager DE STR Biospectrometry Workstation (ABI) from in-gel digested peptides using 10ug/mL modified bovine trypsin in 25mM ammonium bicarbonate. Anti-HSA and anti-human IgG ELISA kits (Bethyl Laboratory) were run according to the manufacture protocol. The...
Trypsin in Biological Products

Porcine TRYPSIN Native from AbD Serotec

Description: NATIVE, ENZYME PORCINE TRYPSIN...
Company:AbD Serotec

Pig Trypsin from BIOPUR

Description: Trypsin (Pig Pancreas) (TPCK Treated, Highly Purified)...
Company:BIOPUR

Trypsin-EDTA (0.05% Trypsin EDTAo4Na)(1X) liquid from Invitrogen

Description: Porcine parvovirus and mycoplasma tested. Contains 0.5 g/L of trypsin (1:250) and 0.2 g/L of EDTA4Na in Hanks' Balanced Salt Solution without CaCl2 MgCl2 6H2O and MgSO4 7H2O. Contains phenol red....
Company:Invitrogen

Rabbit Protein Phosphatase 1 (PP1) from Upstate

Description:...gCl2, 1mM EGTA, 10 Properites: For selective in vitro dephosphorylation of proteins Purification: DEAE chromatography, divalent cation activation plus trypsin digestion, and gel filtration chromatography Quality Assurance: routinely evaluated by dephosphorylation of proteins Company:Upstate

SilverQuest Silver Staining Kit from Invitrogen

Description:...raditional silver stains are eliminated with the SilverQuest kit because the reagents used do not modify protein side chains and do not interfere with trypsin digestion....
Company:Invitrogen

Protein Purification Plate Centrifuge compatible from ABgene

Description:... Quick and simple protocol less than 40 minutes Purifies full-length proteins from SDS-PAGE gels MALDI-TOF compatible Protocol can be followed by trypsin digest if required Plates are centrifuge-compatible Applications N-terminus sequencing Immuno-labelling Protein quantification MALDI-TO...
Company:ABgene

2-D SDS-PAGE Standards, 500 microliter from Bio-Rad

Description:... These 2-D standards consist of seven reduced, denatured proteins (equine myoglobin, soybean trypsin inhibitor, bovine carbonic anhydrase, rabbit muscle GAPDH, bovine muscle actin, BSA, and hen egg white conalbumin) with pIs ranging from 4.5 to 8.5 an...
Company:Bio-Rad

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Trypsin in Biological Definition

Enzyme

...ition in animals relies on digestive enzymes such as salivary amylase , trypsin and chymotrypsin . Their primary role is for the digestion of food and ma...ismutase : transforms superoxide into oxygen and hydrogen peroxide trypsin : digests proteins Tyrosine kinases : those enzymes which transfer phosp...

Proteolysis

...amino acids Conversion of predecessor-proteins (proenzymes , zymogens , prehormones ) into their final structures. Examples of proteases are trypsin and chymotrypsin . ...
Trypsin in Biological Dictionary

Protein array

...rs many protein analysis products. These include in vitro expression systems, protein purification and labeling systems, expression vectors, trypsin and ... In the PISA ( protein in situ array ) method, tagged functional proteins are made ... This enables a protein array to be made in a ...

G-banding

...ining condensed chromosomes. The metaphase chromosomes are treated with trypsin and stained with Giemsa. Dark bands that take up the stain are strongly A,T...l content from favorite medical titles, including Harrison's Online. ... trypsin G-banding stain information including symptoms, causes, diseases, symptom...
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