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Tris in Medical Technology

Tris Pharma Announces Acceptance of Its First Two NDAs

... SOUTH BRUNSWICK, N.J., May 13 /PRNewswire/ -- tris Pharma, a privately owned specialty pharmaceutical...ormulations of a cardiovascular drug. If approved, tris Pharma's liquid and solid dosages will provide an ...ablets, and film strips. Using these technologies, tris Pharma is developing products that are targeted to...
Tris in Medical Products

STACHROM HEPARIN

Description:...omogenic substrate CBS 31.39, ~13 moles/vial, freeze-dried; purified bovine ATIII, freeze-dried; purified bovine factor Xa, 45nKat/vial, freeze-dried; tris buffer....
Company:Diagnostica Stago, Inc.
Tris in Medical Dictionary

Immunofluorescence

...ofluorescence flow cytometry showed that expression of the 1 integrin ... For immunofluorescence staining, sections were quenched for 15 min in tris ... Immunofluorescence microscopy on sections of paraformaldehyde-fixed oocytes was ... Translations of immunofluorescence . immunofluorescenc...
Tris in Biological Technology

Edison Innovation Fund Helps Tris Pharma Expand

New Jersey Provides Support to Emerging Leader in Life Sciences R&D to Ensure Continued Growth TRENTON, N.J., Dec. 6 /PRNewswire/ -- The New Jersey Economic Development Authority (EDA) announced today that Tris Pharma, Inc. will expand its pharmaceutical manufacturing c...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

...modifications to accommodate a 96-well format. 1. The enzyme and buffer solutions were thawed and kept on ice. 2. TE buffer (10 mM tris and 1 mM EDTA in sterile, deionized water, pH adjusted to 7.4) was sterilized by filtration through an 0.2 m filter. 3. The luciferin/luciferas...

Measurement of Molecular Beacons in the SpectraMax Gemini Spectrofluorometer (MaxLine Application Note #36)

...oplates used were Nunc brand, black 384well microplates, surface-treated to minimize binding (cell culture-treated). Allsamples were prepared in 10 mM tris buffer, pH 8, containing 1 mM MgCl 2 , and the final concentrations of beacon and target were 0.3 M and 1.6 M respectively, in a total volume of 54 L....

Evaluation of FlashPlate in a Helicase Assay

...ular Biology Resources Inc., Milwaukee, WI) Assay Buffer (50 mM tris HCl, pH 7.5, 50 mM NaCl, 1 mM DTT, 0.5 mM MnC l 2 , 20% glycerol and 1 mM... 200 μl of PBS at room temperature, and once with 200 μl of 50 mM tris (pH 7.5), 50 mM NaCl at 37C. Each well contained approximately 10,000 to 12...

Fluorescence assay for determination of protease activity

...-HCl buffer, pH 7.8 of which 100 μl was added to the well. After addition of 100 μl substrate, 10 μg/ml, diluted in 10 mmol/l tris HCl buffer, pH 7.8, the plate was incubated overnight at room temperature and then measured. DETECTION The detection was done us...

Coated Plate Immunoassay on the MicroBeta - eg. TSH - IRMA

...aining 0.5% BSA Saturation Buffer formulation: 50 mM tris = 0.606 g tris (Sigma) 0.9% NaCl = 0.9 g conc HCl 0.30 mL ...

Development of an adrenergic α2a receptor binding assay using the LEADseeker Multimodality Imaging System

...cine hydrochloride (Sigma, R104)

Costar™ solid white 384-well microplate (Corning, 3705)

Buffer: 50-mM tris pH 7.4 5-mM MgCl 2 50-mM NaCl

GraphPad Prism™ software v4.0 (GraphPad Software) ...

The development of a serotonin 5-HT2C receptor-binding assay using the LEADseeker Multimodality Imaging System

... 384-well white flat-bottom polystyrene (Corning 3705) not-treated microplate, non-sterile Binding buffer: 50-mM tris pH 7.4, 0.1 % (w/v) ascorbic acid, 10-µM pargyline. GraphPad Prism™ software (GraphPad...

Development of an NK2 neuropeptide receptor binding assay using LEADseeker Multimodality Imaging System

...

Costar™ solid white 384-well microplate (Corning)

Protease-free BSA (Sigma)

Buffer: 50 mM tris pH 7.4 5 mM MgCl 2 2 mM EDTA 0.2% BSA (w/v)

GraphPad Prism&...

Development of a histamine H1 receptor binding assay using the LEADseeker Multimodality Imaging System

...

Protease-free BSA (Sigma, A3059)

Buffer: 50-mM tris pH 7.4 5-mM MgCl 2 0.2% BSA (w/v)

Graphpad Prism™ software v4.0 (GraphPad ...

Tris in Biological Products

Benzonase from Sigma-Aldrich

Description:... Benzonase is a trademark of Merck KGaA. Physical form: Solution in 50% glycerol containing 20 mM tris HCl, pH 8.0, 2 mM MgCl 2 , and 20 mM NaCl Preparation Unit Definition: One unit will digest sonicated herring sperm DNA to acid-soluble oligonucleotid...
Company:Sigma-Aldrich

Mouse Anti-PSD-95, PDZ Domain Monoclonal Antibody, Unconjugated, Clone K28/86.2 from Upstate

Description:... K28/86.2, PDZ Domain GenBank Accession Number : NM_007864 Immunogen : GST fusion to residues 77-299 of human-PSD-95 (PDZ Domain) Formulation: 0.01M tris pH 7.4, 0.075M NaCl containing 0.05% sodium azide Quality Assurance: routinely evaluated by immunoblot on rat brain microsomal protein preparation Rec...
Company:Upstate

UltraClean GelSpin (S) Kit Sample from Mo Bio Laboratories, Inc.

Description:...o the silica spin filter membrane and washed once to remove traces of agarose and salt. The DNA is recovered from the spin filter membrane in water or tris buffer. Rapid Protocol - Purify DNA from agarose gels in as little as 5 minutes. Easy Format - Utilizes a silica spin filter method. Kit rem...
Company:Mo Bio Laboratories, Inc.

B-Phycoerythrin Labeling Kit-NH2 from Abnova Corporation

Description:...other proteins without any activation process. Filtration Tube included in this kit is used for removing small molecules in the sample protein such as tris buffer and amine compounds that interfere with the assay or labeling reaction. The maximum excitation and emission wavelengths of the B-phycoerythrin-...
Company:Abnova Corporation

General DNA Sequencing Services from Polymorphic DNA Technologies, Inc.

Description:...tration method. Templates should be provided in a sequencing reaction compatible buffer. Please submit templates resuspended in deionized water. 10 mM tris is also acceptable, but please do not provide samples in TE. Plasmid DNA should be submitted at a concentration of 0.2 μg per μL. Please pro...
Company:Polymorphic DNA Technologies, Inc.

GenElute Gel Extraction Kit from Sigma-Aldrich

Description:...rane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in tris buffer. The isolated DNA is suitable for a variety of downstream applications, such as automated DNA sequencing, PCR, restriction digestion, cloning, ...
Company:Sigma-Aldrich

UltraClean-htp 96 Well Plant DNA Kit from Mo Bio Laboratories, Inc.

Description:...er (available through Mo Bio). Cells are lysed and DNA is bound to a silica membrane spin filter plate. The DNA is washed and then released into 10 mM tris buffer....
Company:Mo Bio Laboratories, Inc.

UltraClean Plant DNA (S) Kit Sample from Mo Bio Laboratories, Inc.

Description:... vortex. From the lysed cells, the released DNA is bound to a silica spin filter. The filter is washed, and the DNA is recovered in certified DNA-free tris buffer. Pure DNA - Removes polysaccharide and phenolic inhibitors of PCR. High Quality - Yields DNA greater than 50 kb in length. Rapid Pr...
Company:Mo Bio Laboratories, Inc.

10X Automation Buffer from GeneTex

Description:...Saline): -- Deionized or Distilled Water -- 50mM tris Base -- 150mM Sodium Chloride (NaCl) -- 0.02% No...1 Alternatively: -- Substitute 10mM Phosphate for tris Base. -- Substitute 10mM Phosphate for tris Base....
Company:GeneTex

Bovine Phosphatase, Alkaline, Unconjugated from Sigma-Aldrich

Description:... Analysis Note: Assays in tris buffer, pH 10.2 give activity approx. 2X that in the glycine buffer system. Assays in diethanolamine buffer, pH 9.8 give activity approx. 5X that of t...
Company:Sigma-Aldrich
Tris in Biological Definition

Affinity chromatography

...ntibodies to bind to the GST-fusion protein. Elution is achieved by the use of a low pH buffer such as glycine pH 2.8. The eluate is collected into tris buffer pH 8 as low pH will degrade the antibody. This is a nice example as affinity purification is used to purify the initial GST-fusion protein, to...
Tris in Biological Dictionary

Electrophoresis

... Topics for Agarose Gel Electrophoresis . Get Help on your ... An electrophoresis chamber and power supply ... Electrophoresis buffer, usually tris ... Virtual Lab: Agarose Gel Electrophoresis of Restriction Fragments ... High speed electrophoresis in 6 minutes. Length variable electrophor...

Dideoxy Sequencing

... sequencing protocol. ... DIDEOXY SEQUENCING BUFFERS. TM BUFFER. 100mM tris HCl pH8.1. 1M tris HCl Ph8.1. 100ul ... Dideoxynucleotides Dideoxynucleotides , or ddNTPs, ...

Acrylamide gels

...ls and Reagents > Pre-cast Acrylamide Gels . tris GLYCINE. tris TRICINE GELS 10 x10 cm(10-20%, 16%) TBE tris BORATE EDTA GELS ... Other major uses of acr...
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