Transfection

Farnesoid X receptor regulates cystathionase

...enterology addresses this question. The research team led by Dr. Barbara Renga and her colleagues in the University of Perugia, Italy used luciferase transfection assay, Electrophoretic Mobility Shift Assay (EMSA), Chromatin Immunoprecipitation Assay and Real-Time PCR to confirm the role of FXR in the regulation...

UCSF Team Closer to Creating Safe Embryonic-Like Stem Cells

...troducing three viruses, each containing a transcription factor known to induce pluripotency. The transcription factor for c-myc was not included. The transfection involved a simple process in which the tiny microRNA molecules were mixed with a lipid, allowing them to pass through the cell membrane. By labeling t...

UCSF team closer to creating safe embryonic-like stem cells

...troducing three viruses, each containing a transcription factor known to induce pluripotency. The transcription factor for c-myc was not included. The transfection involved a simple process in which the tiny microRNA molecules were mixed with a lipid, allowing them to pass through the cell membrane. By labeling t...

MaxCyte Presents a Panel Discussion With Industry Experts on the Challenges in Developing Cell-Based Screening Assays for Drug Discovery - LIVE Q&A to Follow

...ina. Dr. Reardon reviewed the complexities of cell transfection technologies and challenged the webinar participan...al presentation of the MaxCyte(R) STX(TM) Scalable transfection System. Dr. Brady demonstrated how the MaxCyte STX technology enables the large scale transfection of primary cells, cell lines, and stem cells with ...

MaxCyte to Present Targeted Cancer Immunotherapy Product at 50th Annual Meeting of the American Society of Hematology

...toni's presentation is entitled, "A Highly Efficient, Clinically Applicable transfection Method to Redirect the Specificity of Immune Cells and Enhance Their Anti-T...led expertise to the global leaders in cell-based therapies. MaxCyte's cell transfection technology platform enables the discovery, development, manufacturing a...

A potential approach to treatment of hepatitis B virus infection

...to replication stress. They investigated whether the inhibition of DNA damage response by CF, TP and UCN01 or the restoration of p21 expression by p21 transfection or proteasome inhibition would lead to suppressed HBV replication. They set up a chronic HBV infection model by culturing hepatocyte HL7702 cells with...

A potential route for human tumor gene therapy

... membranous ion exchange protein in gastric carcinoma cells, and the effects on the biological behaviors of gastric carcinoma cells. The purpose of transfection of the antisense NHE1 gene into the human gastric carcinoma cell line SGC-7901 is to investigate the effects of antisense therapy targeting the NHE1 g...

Cellectricon Launches World's First System for High Throughput RNAi Screening

... The world's first fully automated high throughput transfection system enables reagent-free delivery of genetic ma... system on the market allowing for high throughput transfection of cells in culture directly in 384-well microplat...acceptance targeting the ion channel screening and transfection markets. The company utilizes state-of-the-art int...

Sigma-Aldrich Announces mirPremier(TM) microRNA Isolation Kit for miRNA Purification

...s and AQUA peptides for protein level detection as well as an entire workflow of supporting reagents (cell culture, cell biology compounds and assays, transfection reagents, and much more). For more information, visit http://www.sigma.com/rnai . About Sigma-Aldrich: Sigma-Aldrich is a leading Life Science and H...

The new source of islet cells

...nefit to diabetic patients. The article describes the differentiation of rat pancreatic ductal epithelial cells into insulin-producing cells by the transfection of PDX-1. In recent years, though great efforts have been made to differentiate embryonic stem cells, pancreatic ductal epithelial multipotent progeni...

Transfection in Medical Products

GENios Plus multi label reader

Description:...iate fluorescence labels. As a multi-detection system, GENios will also offer superior performance for additional applications such as expression and transfection studies (GFP and Luciferase), enzymatic assays (kinetic), toxicology or environmental investigations. For cell based assays, GENios allows you to dete...
Company:Tecan

Genios Multi-Detection Microplate Reader

Description:...iate fluorescence labels. As a multi-detection system, GENios will also offer superior performance for additional applications such as expression and transfection studies (GFP and Luciferase), enzymatic assays (kinetic), toxicology or environmental investigations. For cell based assays, GENios allows you to dete...
Company:Tecan

Transfection in Biological News

Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols

...b_pages/People/krull.htm ) provides a method for stable integration and reliable long-term expression of a transgene. Sleeping Beauty transposon-based transfection is a two-component system consisting of a transposase and a transposon containing inverted repeat/direct repeat sequences that result in precise integ...

Scientists eye secrets of retinal regeneration

...ng a gene-based method called "retinal transfection," to gain a more accurate picture of outer segment growth in rat retinas. "Basically, retinal transfection means introducing different genes into the eye to switch particular cellular functions on or off," Dr. Chuang explains. After a variety of these a...

Buckyballs used as 'passkey' into cancer cells

...rcent of pediatric cancer deaths," said Jianhua Yang. "Our findings are significant because neuroblastoma cells are well-known for their difficulty in transfection through the cell membrane." Barron is Rice's Charles W. Duncan Jr.-Welch Professor of Chemistry, professor of materials science and associate dea...

New culture method for hepatitis C virus uses primary hepatocytes and patient serum

...ith different HCV strains were added to the hepatocyte culture system, viral replication occurred and new virus particles were produced. In both transfection and infection models, virus particles were released in a cyclical manner, with bursts of virus produced every 10-14 days. This is similar to what has ...

What's next for gene therapy? Plastic

...ermit efficient release of the DNA for protein synthesis." Layman will deliver the talk, "Influence of macromolecular architecture on nucleic acid transfection (POLY 611)," at 4 p.m., Wednesday, Sept. 13, in the Marriott Salon 14/15 as part of the International Biorelated Polymers Symposium. Authors are Layma...

Transfection in Biological Technology

Clontech Laboratories, Inc. Releases the Xfect(TM) and Xfect(TM) Stem Transfection Reagents

MOUNTAIN VIEW, Calif., May 28 /PRNewswire/ -- Clontech Laboratories, Inc., a wholly-owned subsidiary of Takara Bio Inc., today announced the upcoming (June 1, 2009) release of the Xfect(TM) and Xfect(TM) Stem transfection reagents. These novel transfection reagents are based on biodegradable nanop...

MaxCyte Introduces the GT(TM) Flow Transfection System for Application with Autologous and Allogeneic Stem Cell Therapies

Technology enhances biological activity (regenerative potential) of stem cells and accelerates development of proprietary enhanced therapeutics GAITHERSBURG, Md., Feb. 17 /PRNewswire-USNewswire/ -- MaxCyte announces today the introduction of its GT(TM) Flow Transfection System at the 4th Annu...

The MaxCyte(R) STX(TM) Scalable Transfection System to be Presented at the Rapid Fire Innovation Session at LabAutomation2009

GAITHERSBURG, Md., Jan. 26 /PRNewswire/ -- MaxCyte, Inc., the pioneer in scalable, high performance cell loading systems, announces the presentation of the MaxCyte(R) STX(TM) Scalable Transfection System at LabAutomation2009 in the Rapid Fire Innovation Session, a joint program of the Association ...

Altogen Biosystems Launches New Nanoparticle-based In Vivo Transfection Reagent

LAS VEGAS, Nov. 3 /PRNewswire/ -- Altogen Biosystems (http://www.altogen.com ) announced launch of novel Nanoparticle-based transfection reagent for use in laboratories and research facilities for animal testing. Transfection is one of the major laboratory methods used to introduce DNA and RN...

The MaxCyte STX Scalable Transfection System to be Launched at the SBS Symposium on Cell-Based Assays

GAITHERSBURG, Md., Oct. 22 /PRNewswire/ -- MaxCyte, Inc., the pioneer in scalable, high performance cell loading systems, announces that it will be launching the MaxCyte STX Scalable Transfection System at the Society for Biomolecular Sciences (SBS) Symposium "Cell Based Assays: Innovations in...

MaxCyte Debuts the STX Scalable Transfection System at the A/C/T Assays & Cellular Targets Meeting

GAITHERSBURG, Md., Sept. 23 /PRNewswire/ -- MaxCyte, Inc., the pioneer in scalable, high performance cell loading systems, announces that they will be conducting a Product Demonstration of the MaxCyte STX Scalable Transfection System on Thursday, September 25, 2008, at 10:00AM PDT, at the A/C/...

Altogen Biosystems Launches Custom Biotechnology Research Services: Generation of Stable Cell Lines and RNAi Cell Transfection Services

LAS VEGAS, March 11 /PRNewswire/ -- Altogen Biosystems ( http://www.altogen.com ) announced launch of new contract research services. Located in Las Vegas, NV, Altogen Custom Services focus on providing specialized biotechnology and pharmaceutical services, including RNA Interference (RNAi) se...

Sigma-Aldrich Introduces N-TER Nanoparticle siRNA Transfection System for Delivery of siRNA Into Difficult-to-Transfect Cell Types

ST. LOUIS, Oct. 18 /PRNewswire-FirstCall/ -- Sigma-Aldrich (Nasdaq: SIAL) announced today the global release of the N-TER(TM) Nanoparticle siRNA Transfection System. N-TER allows researchers to reproducibly interrogate gene function, via siRNA transfection, in model cell types that have previo...

Biolistic Transfection of Organotypic Brain Slices

A. Kimberley McAllister, Nina Tang, Lawrence C. Katz, and Donald C. Lo. Department of Neurobiology, Box 3209, Duke University Medical Center, Durham, NC 27710 Introduction The introduction of genetic material ...

Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities

Kathleen A. Maguire-Zeiss, Brandon K. Harv e y, Rita E. Giuliano and H o w a rd J. Federo ff, University of Rochester, Rochester, NY, USA Introduction Transfection efficiency is dependent upon a number of factors, including method of transfection...

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Transfection in Biological Products

BacVector-3000 Transfection Kit from Novagen

Description: ...
Company:Novagen

BD BaculoGoldTM Transfection Kit 5 transfections from BD Biosciences Pharmingen

Description: BD BaculoGoldTM Transfection Kit 5 transfections...
Company:BD Biosciences Pharmingen

TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation

Description: Request Info...
Company:Mirus Bio Corporation

Calcium Phosphate Transfection Kit from Invitrogen

Description: Invitrogen's Calcium Phosphate Transfection Kit provides high-quality reagents to enable the introduction of DNA into tissue culture cells via calcium phosphate co-precipitation....
Company:Invitrogen

Silencer CellReady siRNA Transfection Optimization Kit from Ambion

Description: Prior to siRNA library screening, optimal siRNA transfection parameters must be identified and verified for each cell type used. The Silencer CellReady siRNA Transfection Optimization Kit, which was developed specifically for optimizing transfection conditions in 96 well plates, dramatically simpli...
Company:Ambion

ProteoJuice Protein Transfection Reagent from Novagen

Description: Introduction of a protein into cells permits studies of protein-protein interactions, protein turnover, cell signaling pathways, apoptotic pathways, and transcription factor-mediated gene regulation. The most rapid method for studying the effects of a specific protein in a mammalian cell is to tran...
Company:Novagen

siPORT NeoFX Transfection Agent from Ambion

Description: Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT NeoFX Transfection Agent refines siRNA transfection protocols resulting in less optimization. siPORT NeoFXs lipid-based formulation can be used to efficiently transfect adherent cells as they are...
Company:Ambion

siPORT NeoFX Transfection Agent 1 ml from Ambion

Description: Gene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT NeoFX Transfection Agent refines siRNA transfection protocols resulting in less optimization. siPORT NeoFXs lipid-based formulation can be used to efficiently transfect adherent cells as they are...
Company:Ambion

TransIT-TKO siRNA Transfection Reagent from Mirus Bio Corporation

Description: Designed specifically for high efficiency siRNA delivery in mammalian cells. Low toxicity reagent proven to work effectively in a wide range of cell types. Results in silencing of greater than 95% of target gene expression. One step, serum compatible reagent supplied with an optimized protocol....
Company:Mirus Bio Corporation

TransIT-siQUEST Transfection Reagent from Mirus Bio Corporation

Description: Cellular uptake of long double stranded RNA (dsRNA) has been shown to induce RNA interference in a diverse group of organisms as well as insect cells in culture. RNA interference leads to the inhibition of protein expression by utilizing sequence-specific, dsRNA-mediated destruction of the target m...
Company:Mirus Bio Corporation

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Transfection in Biological Definition

Molecular biology

...l cells, is called transfection. Several different transfection techniques are available, including calcium phosphate transfection, liposome transfection , and proprietary transfection reagents such as Fugene. DNA can also be introduce...

Transfection in Biological Dictionary

Transfection

Transfection is the process of introducing nucleic acids into cells by non-viral methods. The term "transformation" is preferred to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells; "transduction" is often used to describe virus-mediated DNA......

Transient transfection

Transfection is the process of introducing nucleic acids into cells by non-viral methods. The term "transformation" is preferred to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells; "transduction" is often used to describe virus-mediated DNA......

Stable transfection

Transfection is the process of introducing nucleic acids into cells by non-viral methods. The term "transformation" is preferred to describe non-viral DNA transfer in bacteria and non-animal eukaryotic cells; "transduction" is often used to describe virus-mediated DNA......

Transcapsidation

...This transcapsidation approach consisted of the transfection of pairwise ... Transcapsidation between subgro...This transcapsidation approach consisted of the transfection of pairwise ... virus groups determines vecto...This transcapsidation approach consisted of the transfection of pairwise ... Transcapsidation ,biological ...

SiRNA

... Predesigned siRNA and siRNA libraries designed to reduce off-target effects and increase RNAi performance. Custom siRNA synthesis services and transfection reagents. - - Online guide for performing RNAi experiments using synthetic siRNA molecules. ... We used to select siRNA sequences with TT in...

Reporter Gene

...luorescent Protein (GFP) Reporter Gene System ... Human Growth Hormone (hGH) Reporter Gene System ... Products > Genetic Reporters & transfection > Applications of Reporter Gene Assays ... Using Bioluminescent Reporter Genes to Optimize shRNA Target Sites for ... Detect reporter ...

In vivo

...s annual sketch comedy show. ... This is a very special year for In Vivo as we will be celebrating the show's 30th anniversary! ... In Vivo transfection Agent :: siRNA and DNA Delivery Invivo ... siRNA and plasmid DNA in vivo delivery reagent. Modes of administration: ... What is in vivo ? ...

Genomic DNA

... Human genomic DNA matched pair products for tumor specific gene & tumor ... DNA Kits. Full Length cDNA Clones. Genomic DNA . GFC transfection Arrays. Matched Pair cDNA ... PCR-Ready Genomic DNA - Plant Normal Tissue: Arabidopsis. BioChain. 10 µg ... Apple Genomic DNA . Zyagen Labor...

Electroporation

... vaccine therapeutics and delivery systems, ... mechanism of electroporation is not ... The world leader in the field of electroporation and transfection offering an extensive line of products and services for the laboratory research market. ECM 830 system for electroporation of mammalian cells ...

CDNA array

.... TrueORF cDNA Clones. Destination Vector. TrueClone Human Collection ... transfection Array - 704 transfection ready cDNA plasmids ... High throughput over expression assay for f...