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Transfected in Medical News

Vaporized viral vector shows promise in anti-cancer gene therapy

...chose a lentiviral vector, derived from a retrovirus and known for its ability to infect nondividing cells and effect persistent genetic changes. They transfected the lentiviral vector with a negative regulator of Akt signaling, carboxyl-terminal modulator protein (CTMP), which would theoretically inhibit Akt si...

p90RSK: A new therapeutic target for liver fibrosis?

...sue, p90RSK was over-expressed and located in activated HSC, which had a significant positive correlation with collagen type I levels. In HSC-T6 cells transfected with RNAi targeted to p90RSK, the expression of collagen type I was down regulated according with that of p90RSK. However, collagen type I promoter ac...

Cardium Reports on Recent Highlights, Financial Results and 2009 Outlook

...bed the potential use of stem cells, such as mesenchymal stem cells or MSCs transfected with an Ad5IGF-1 vector (such as that used in Corgentin(TM)), for addressin...nati showed in a preclinical model of heart attack that MSCs that had been transfected with Ad5IGF-1 were particularly effective at promoting angiogenesis within ...

What are protective effects of anti-ricin A-chain aptamer?

...ry effects of ricin on translation inhibition in cell-free and cell-based luciferase assays and ricin-induced cytotoxicity assay. The use of a stably transfected cell-based luciferase assay will facilitate the development of high throughput screening for inhibitors of ricin as potential antidotes for the treatm...

MaxCyte Presents a Panel Discussion With Industry Experts on the Challenges in Developing Cell-Based Screening Assays for Drug Discovery - LIVE Q&A to Follow

...technology for cell modification for both research and larger scale cell-based applications in drug discovery. The MaxCyte STX can produce billions of transfected cells in minutes compared to current time-consuming and inconsistent methods. This is possible even for large scale transfection of primary cell lines...

How to select anti-hepatitis B virus agents for drug-resistance patients?

...The recombinant plasmids containing 1.1 copies of HBV genome were transient transfected into Huh7 cells and antiviral susceptibility of lamivudine and adefovir wer...cloned into HBV expression vector, and recombinant plasmids were transcient transfected into Huh7 cells. The results indicated that HBV genome of clinical HBV iso...

Fireflies' glow helps UT Southwestern researchers track cancer drug's effectiveness

... feed tumors. They tested their theory in mice bearing human breast-cancer tumors. Before being introduced to the animals, the tumor cells had been transfected with the firefly gene, which becomes part of the cells as they divide and grow just like genetically modified, herbicide-resistant food crops. For ...

Scripps Florida scientists develop a process to disrupt hepatitis C virion production

... Then they generated a clone of the virus, transcribed the RNA from that clone, and purified the RNA. This RNA, which is directly infectious, was then transfected into a liver cell line where it produced all the HCV proteins that are encoded by that RNA genome. Those proteins assemble in the cell to make a s...

3 newly discovered ERK pathway proteins related to CagA induced disease

... laser desorption / ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technology, to study the global protein expression changes in AGS cells transfected with CagA gene. As various researches indicated a relationship among CagA, ERK/MAPK pathway activation and gastric cancer, the article further investi...

The new source of islet cells

...ase the amount of insulin-releasing, Dr Liu et al. transfected PDX-1 into primary pancreatic ductal epithelial cells and then differentiated the transfected cells into insulin-producing cells. In contrast, t...nd insulin mRNA and protein were detectable in the transfected cells. Endogenous PDX-1 might play an important ro...
Transfected in Medical Technology

DNA Therapeutics Reports a Novel Mechanism of Action and a Biomarker of its Lead Molecule Dbait That Open an Innovative Pathway-Targeting in Molecular Cancer Therapy

...sists more than 6 days as compared to 1 day for irradiation. Cell imaging shows that the global distribution of gamma-H2AX in the nucleus of the cells transfected by Dbait was radically different of the gamma-H2AX foci organization observed when H2AX phosphorylation was triggered by irradiation alone. This indic...
Transfected in Medical Products

hGH ELISA

Description:...of a gene family. The hGH ELISA is used to quantitatively measure the expression of hGH released into the cell culture supernatant of eukaryotic cells transfected with a plasmid containing a reporter gene encoding for hGH. hGH Elisa is standardized, highly sensitive, specific, fast, no leaching, comparable, easy...
Company:Roche Diagnostics
Transfected in Biological News

Simple recipe turns human skin cells into embryonic stem cell-like cells

...dentity. The researchers have now shown that the same four factors can generate iPS cells from fibroblasts taken from human skin. From about 50,000 transfected human cells, we obtained approximately 10 iPS cell clones, Yamanaka said. This efficiency may sound very low, but it means that from one experiment, w...

Enzyme delivered in smaller package protects cells from radiation damage

...inicircle" plasmid. To determine if the cells transfected with the MnSOD minicircle plasmid retained radiopr...rradiated those cells as well as another cell line transfected with MnSOD in a full-sized plasmid. They also irradiated a parent mouse cell line that had not been transfected with MnSOD. After irradiation, the cells were plat...

New culture method for hepatitis C virus uses primary hepatocytes and patient serum

...nes. The related report by Lázaro et al, "Hepatitis C virus replication in transfected and serum-infected cultured human fetal hepatocytes," appears in the Februa...o infect new cells. In their first experiments, Fausto and colleagues transfected hepatocyte cultures with HCV genomic RNA and found replication of HCV RNA g...

Dying of excitement

...d a binding site for the activating transcription factor AP-2 into a binding site for the repressor GCF2 -- a swap that inhibited promoter activity in transfected rat brain cells. Whether the mutant promoter decreases EAAT2 expression in the human brain, as would be predicted, remains to be tested. ...
Transfected in Biological Technology

The MaxCyte(R) STX(TM) Scalable Transfection System to be Presented at the Rapid Fire Innovation Session at LabAutomation2009

...ction technology. Using the MaxCyte STX, more than ten billion cells can be transfected in less than 30 minutes with one or more loading agents at efficiencies and...d CEO, Doug Doerfler. "The MaxCyte STX can produce billions of high quality transfected cells in minutes compared to time consuming and inconsistent alternative me...

MaxCyte Hosts Live Discussion with Industry Experts on the Challenges in Developing Cell-Based Screening Assays for Drug Discovery

...logy for cell modification for both research and larger scale cell-based applications in drug discovery. The MaxCyte STX can produce a large number of transfected cells in minutes compared to current time-consuming and inconsistent methods. More assays can be developed and performed in a shorter period of time, ...

MaxCyte Debuts the STX Scalable Transfection System at the A/C/T Assays & Cellular Targets Meeting

...chnology as key to reducing drug discovery bottlenecks," says MaxCyte President and CEO, Doug Doerfler. "The MaxCyte STX can produce a large number of transfected cells in minutes compared to current time-consuming and inconsistent methods. Cells modified using the MaxCyte STX can be used on the research scale a...

Promegas Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar

...terials and Methods HEK293 cells stably transfected with a CRE/CL1 hPEST Renilla construct ...olume 384 assay format, a density of 12,000 stably transfected HEK 293 cells per well was plated with ... 1536-well assay format, a density of 4,000 stably transfected HEK 293 cells per well was plated with ...

HTRF IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers

... chimeric constructions. The IP-One assay showed equally good performance on different cellular backgrounds, stable or transient transfected cells, and chimeric constructs. There is a strong correlation with reference methods and no cross reactivity with 50 M of the fo...

Gq-coupled receptor assays: a comparison of FLEXstation and FLIPR

...nd these proteins are popular drug targets for biotechnology and pharmaceutical companies. The cell line used in these studies, M1 CHO, is stably transfected with the M1 muscarinic receptor. This transmembrane protein is a G q coupled acetylcholine receptor. It is stimulated with carbachol, which leads to t...

Gq-coupled receptor assays using FLEXstation: M1 muscarinic receptor

...iseases. Therefore, it is important to compare receptor function in normal and disease states. The cell line used in these studies, M1 CHO, is stably transfected with the M1 muscarinic G q coupled receptor 1 . This receptor is stimulated with carbachol, which leads to the release of calcium from the endoplasmic...

FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses

...following examples, several GPCRs were transiently transfected into human embryonic kidney cells (HEK-293) at non...oximate density of 3x10 6 in 10 cm 2 dishes were transfected using the Lipofectamine Plus reagent and 24 hours ...12,000 can be achieved with FLUO-4 using HEK cells transfected with the same construct under optimum cell seeding...

Measuring siRNA-mediated knockdown of IL-8 mRNA

...mine 2000 (Invitrogen) transfection mix. The media bathing the HeLa cells was replaced by the siRNA/Lipofectamine mix to a volume of 175 l. Cells were transfected at 37C for four hours, followed by recovery for either four or eight hours in 100 l fresh complete DMEM. The QuantiGene Lysis Working Reagent was adde...

Measurement of Green Fluorescent Protein in the SpectraMax Gemini XS Spectrofluorometer (MaxLine Application Note #44)

...ETHODS Human embryonic kidney 293 cells (HEK-293) were transiently transfected with a wild-type GFP expression plasmid (pS65T, Clontech, discontinued prod...ini-XS is able to reliably detect the 1.2-fold increase in signal over mock transfected cells. Because the SPECTRAmax GEMINI-XS is able to monitor up to four diffe...
Transfected in Biological Products

Flp-In pcDNA5/FRT Complete Kit from Invitrogen

Description:... Since the Flp-In System creates isogenic cell lines all transfected clones produce equivalent levels of the protein of interest. This homogeneo... chloramphenicol-acetyl transferase (CAT) was subcloned into pcDNA5/FRT and transfected into Flp-In-293 cells. Western blot analysis of individual hygromycin-resis...
Company:Invitrogen

Flp-In pcDNA5/FRT Core Kit from Invitrogen

Description:... Since the Flp-In System creates isogenic cell lines all transfected clones produce equivalent levels of the protein of interest. This homogeneo... chloramphenicol-acetyl transferase (CAT) was subcloned into pcDNA5/FRT and transfected into Flp-In-293 cells. Western blot analysis of individual hygromycin-resis...
Company:Invitrogen

Anti-IRF7 Polyclonal Antibody, Unconjugated from Invitrogen

Description:...s-react with other related IRF proteins. Reactivity of this antibody with murine IRF7 protein was confirmed by western blot analysis of the 293T cells transfected with IRF7. Reactivity with endogenous protein was not observed...
Company:Invitrogen

Rabbit Anti-Human SENP1 Monoclonal Antibody,, Clone ZMD.366 from Invitrogen

Description:...egion of human SENP1. Specificity: Specific for the human ~73 kDa SENP1. Reactivity: Human (positive controls: (DYKDDDK or DYKDDDDK)-HA-tagged-SENP1 transfected Hep2 cell lysates HL-60 cell lysates). Applications: Western blotting Immunoprecipitation...
Company:Invitrogen

Anti-Vesicular Monoamine Transporter 2 (VMAT2) Polyclonal Antibody, Unconjugated from CHEMICON

Description:... Monoamine Transporter 2 (VMAT2). An antibody made to the C-terminal VMAT2 peptide detected a major band at ~55 kDa in postnuclear supernatants of CHO transfected with VMAT2 and not in wild type cells (2). Some additional bands, both higher and lower molecular weight, were also detected and remain unaffected by ...
Company:CHEMICON

Luciferase Reporter Assay Kit from MBL International

Description:...rter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg2+ and buffer are added to the l...
Company:MBL International

ApoBrdU Red DNA Fragmentation Kit from MBL International

Description:...dure provides greater sensitivity than biotinylated or digoxigenylated methods. Results can be analyzed by flow cytometry. The kit works well with GFP transfected cells Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. MBLs Apo-BrdU In Situ DNA Fragmentation Assay Kit provides c...
Company:MBL International

gamma-Secretase Inhibitor IV, Unconjugated from Calbiochem

Description:...amma;-secretase. Equipotently inhibits the release of Aβ x-40 (ED 50 = 2.6 μM) and Aβ x-42 (ED 50 = 2.7 μM) in HEK293 cells stably transfected with the Amyloid Precursor Protein Swedish mutants. Purity: ≥95% by HPLC.. Soluble in DMSO. M.W. 402.5. 2-Naphthoyl-Val-Phe-CHO...
Company:Calbiochem

Cell Line Nucleofector Kit T from amaxa biosystems

Description:...cluding the nucleofection parameters. Nucleofector Kits are only functional in combination with the Nucleofector Device. Different cell lines can be transfected with this kit. The corresponding efficiencies and viabilities are given in brackets in percent (Efficiency [%] / viability [%]). Cell Lines: A549...
Company:amaxa biosystems

Rat Anti-Mouse NKG2A / C/ E Monoclonal Antibody, Unconjugated, Clone 20d5 from Abcam

Description:... Rat monoclonal [20d5] to NKG2A + C+ E ( Abpromise for all tested applications). Antigen: Tissue / cell preparation (Mouse): CHO transfected cells expressing the B6 allele of NKG2A. Entrez Gene ID: 3821 Swiss Protein ID: P26715...
Company:Abcam
Transfected in Biological Definition

Gene therapy

...etroviral and other recombination-based approaches, a second problem arises in the unpredictablity of where the new DNA inserts into the chromsomes of transfected cells. If the gene is inserted in a bad place — for example within the sequence of an important gene, or within non-coding ( intron ) regions ...
Transfected in Biological Dictionary

Stable transfection

...ribe virus-mediated DNA... Stable Transfection . Transient and Stable Transfection ... effect is a stable or transient transfection , the transfected genetic material ... Altogen Biosystems - Cell Transfection Resource ... Transient and Stable Transfection . Cancer Cell Lines and Primary C...

SiRNA

...hibit MEK1 expression using RNA interference, a method in which ... siRNA Products ... Chromosome DBase siRNA shRNA Plasmids shRNA Lentiviral transfected Lysates Flow ... Biotechnology now offers siRNA gene silencers targeted ... Small interfering RNA Mediating RNA interference in cultured mamm...

Reporter Gene

...n, from ... Introduction to Reporter Gene Assays Introduction to Bioluminescence Assays The ... Hi. I know how the reporter gene works in a transfected cell. ... of one of the most famous reporter genes , GFP, was awarded the Nobel prize last year. ... PET-based reporter gene imaging was dev...

Polyprotein

...d nonstructural ... Polyprotein processing activity of the NS3 protease is required for NS4A ... ... at the N terminus of the polyprotein and transfected the derived T7 RNA ... reading frame codes for a polyprotein from which structural proteins VP0, VP3, ... This polyprotein contained amino...

Heteroduplex Dna

...ated ... DNA replication through the heteroduplex recombination intermediate generates ... of single-stranded loops in heteroduplex DNA transfected into mammalian cells. ... Cleavage of Mispaired Heteroduplex DNA Substrates by Numerous Restriction Enzymes 1 ... ability to cleave mispaired ...

Hepatocytes

...tocytes , animal hepatocytes , ... Incubation of rat hepatocytes with Hcy (50–200 µ) caused a significant increase ... When hepatocytes were transfected with decoy AP-1 oligodeoxynucleotide to ... Human hepatocytes generated in this model retained functional properties of ... To identify human ...

Fusion Protein

...mice ... Immunohistochemical detection of His•Tag fusion proteins in transfected COS-1 cells ... Tag firefly luciferase (Fluc) fusion protein was transiently transfected into ... Affinity purification of GST fusion proteins ... PopCulture...

Exogenous DNA

...eloped under NSF SGER ... and Exogenous DNA -Free Sampling ... ... expression of the exogenous DNA in the primary cells, thereby producing transfected primary cells which express the exogenous DNA encoding erythropoietin; ... Bacterial transformation involves bacteria incorporating ex...
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