Navigation Links
transcriptases in Biological News

Biochemists report discovery of structure of major piece of telomerase; implications for cancer

...igon's laboratory has been working on the RNA. "It is a dream of mine to figure out what this RNA is doing with the protein," Feigon said. "Reverse transcriptases normally copy RNA to DNA, but do not contain RNA; in this enzyme, the protein requires the RNA component to function. The enzyme is unique because it ...
transcriptases in Biological Technology

Reverse transcriptases suitable for two-step RT-PCRd

Product size Type of primers Primer anneal. temp. Sensitivity e Specificity f Fidelity in RT GC-rich structures ...

Reverse transcriptases suitable for one-step RT-PCRb

Product size Type of primers Primer anneal. temp. Sensitivity Specifi- city Fidelity in PCR Tolerance ...

Properties of Reverse Transcriptases

Enzyme Maximum size of template Temperature optimum Units needed for standard reaction Ion require- ment Rnase H activity ...

Suggested applications for reverse transcriptases

Enzyme Full-Length cDNA High fidelity cDNA for cloning Amplification of viral RNA DDDRT a RACE a ...

Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter

...iological activity. These assays measure the inhibitory effect of test compounds on target enzymes such as HIV proteases, various kinases, RNA and DNA transcriptases and polymerases. Enzyme activity is often determined by measuring the conversion of a radiolabeled substrate to a product that can be separated from t...

RNA Amplification Just Got Better: MessageAmp II

... yields of full-length cDNA compared to other reverse transcriptases (Figure 1). Figure 2 demonstrates that up to twice as ...ild type M-MLV, AMV, or other engineered M-MLV reverse transcriptases when low amounts of total RNA are used (~100 ng). This...

HIGH SENSITIVITY qRT-PCR

...ore sensitive than SuperScript II and other RNase H MMLV reverse transcriptases Linear quantitation of mRNA targets over a 106-fol...sitivity in RT-PCR RNase H minus (RNase H ) reverse transcriptases (RTs) are a popular choice among researchers for appli...

Better RNA Amplification

...ll-length, double-stranded cDNA templates. ArrayScript produces the highest yields of full-length cDNA compared to other reverse transcriptases (Figure 1). The second-strand cDNA synthesis reaction has been optimized for maximal conversion of first-strand cDNA into full-l...

ArrayScript RT: Maximize Yields of Full-length cDNA

...ed using wild type M-MLV, AMV, or other engineered M-MLV reverse transcriptases (Figure 2). When starting with low amounts of total RNA, ArraySc...or all RT Applications Reverse transcriptases are also used in RT-PCR, cDNA library construction, an...

Choice of RT-PCR Enzymes

...lse priming. Thus, thermoactive reverse transcriptases that can be incubated at high temperatures (5070C)...rast to Tth DNA Polymerase, AMV and M-MuLV Reverse transcriptases have RNase H activity. This activity wi...nt ion requirement Most reverse transcriptases require a divalent ion for activity. Enzy...
transcriptases in Biological Dictionary

RT-PCR

...te as a ... Amplifying RNA with RT - PCR . Overview. The use of reverse transcriptases to perform first-strand ... are one-tube, one-step, coupled RT - PCR Syste... RT - PCR System saves you four pipetting steps ... cDNA Synthesis Reverse transcriptases Reverse Transcription Systems RT - PCR RNase Inhibitors ... Methods, p...

Reverse transcription

...erse Transcription System, visit: ... The ImProm-II™ Reverse Transcription System is excellent for reproducible ... cDNA Synthesis Reverse transcriptases Reverse Transcription Systems RT-PCR RNase ... ied the reverse transcription reaction and its conse ... and gene-specific reverse transc...

Group I introns

...mains I and IV. ... Group II introns are self-splicing ribozymes believed to be the ancestors of ... Many group II introns encode reverse transcriptases that promote both RNA ... Group ,II, introns ,biological ,biology dictionary,biology terminology,biology terms,biology abbreviations ... Not all ...

Group II introns

...ice without ... Group II introns are self-splicing ribozymes believed to be the ancestors of ... Many group II introns encode reverse transcriptases that promote both RNA ... Global Regulators Orchestrate Group II Intron Retromobility ... Retrohoming: cDNA-Mediated Mobility of Group II ...
Other Tags
(Date:7/27/2015)... ... ... Dr. Robert G. Schwartz , principal of Greenville, SC-based Piedmont Physical ... Centers in order to offer his patients a new system of care utilizing laser ... , Peripheral neuropathy is a disease caused by damage to the peripheral nerves, and ...
(Date:7/27/2015)... , ... July 28, 2015 , ... Combination Product GMP ... – 3:00 p.m. EDT, http:// http://www.fdanews.com/comboproductgmpcompliance , Manufacturing a drug ... of combination products things can get messy in a hurry. In a 46-page draft ...
(Date:7/27/2015)... ... July 28, 2015 , ... Recently, the Anderson ... medicine provider for the entire school district including Anderson County Middle School (ACMS) ... Rick Sallee, “Student safety and well-being are a top priority for us in ...
(Date:7/27/2015)... ... July 28, 2015 , ... The National Association of Professional Women ... VIP Woman of the Year Circle. She is recognized with this prestigious distinction for ... NAPW is the nation’s leading networking organization exclusively for professional women. , “I’m pleased ...
(Date:7/27/2015)... Garden City, NY (PRWEB) , ... July 28, 2015 , ... ... as a 2015-2016 inductee into the NAPW VIP Woman of the Year Circle. ... 700,000 members and over 200 operating Local Chapters, NAPW is the nation’s leading networking ...
Breaking Medicine News(10 mins):Health News:Piedmont Physical Medicine & Rehabilitation, P.A. Partners with Realief® Neuropathy Centers to Offer Peripheral Neuropathy Laser Treatment 2Health News:FDAnews Announces — Combination Product GMP Compliance: Understanding FDA’s New Streamline Approach Webinar, Aug. 19, 2015 2Health News:FDAnews Announces — Combination Product GMP Compliance: Understanding FDA’s New Streamline Approach Webinar, Aug. 19, 2015 3Health News:FDAnews Announces — Combination Product GMP Compliance: Understanding FDA’s New Streamline Approach Webinar, Aug. 19, 2015 4Health News:KORT Physical Therapy Named Sports Medicine Provider for Anderson County School System 2Health News:KORT Physical Therapy Named Sports Medicine Provider for Anderson County School System 3Health News:NAPW Inducts Deana Skidmore, Assistant Director of Nursing at Wilton Meadows Rehablilitation and Health Care Center, Into its VIP Woman of the Year Circle 2Health News:NAPW Inducts Jane Simmons, Counseling Intern at Omar Bradley Middle School of the Northeast Independent School District , Into its VIP Woman of the Year Circle 2
(Date:7/2/2015)... Fingerprint Cards has received an order for its ... distributor World Peace Industrial Group (WPI), part of WPG Holdings. ... . Deliveries are planned to take place during Q3 ... manufacturers in Asia . The order value ... of approximately 2,200 MSEK for 2015. Jörgen Lantto, ...
(Date:6/30/2015)... June 30, 2015 Genisphere announced ... CEO to help further develop Genisphere,s therapeutics capabilities and ... experience, having spent much of the last 25 years ... Baxter Bioscience, and Johnson & Johnson. Tom started his ... and Biotech Corporate Finance. He graduated from Dartmouth College ...
(Date:6/26/2015)... ATL Technology, LLC, a top provider of electromechanical ... use solutions, headquartered in Springville, Utah , ... California corporation with locations in Santa ... Rica ). This acquisition will incorporate MedConx,s ... ATL Technology,s existing facility in Costa Rica ...
Breaking Biology News(10 mins):FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2Genisphere Appoints New CEO, Thomas Bliss 2Genisphere Appoints New CEO, Thomas Bliss 3ATL Technology Announces Acquisition of MedConx, Inc. and Expanded Global Footprint 2
Other Contents