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Thiols in Biological News

Nonstick and laser-safe gold aids laser trapping of biomolecules

...ts are easy to lay down in desired patterns at the nanometer scale. Perkins group attached the DNA to the gold with sulfur-based chemical units called thiols (widely used in nanotechnology), an approach that is mechanically stronger than the protein-based bonding techniques typically used in biology. The JI...

Progress toward an antitumor vaccine

... spacer, which has an allyl group (two carbon atoms attached by a double bond) at its end. The glycopeptide is coupled to a building block that causes thiols (sulfur–hydrogen groups) to protrude from the molecule. In the next, light-initiated (photochemical) reaction, only the desired thioether bonds are fo...
Thiols in Biological Technology

Modification of Bio-Rad DC Protein Assay for Use With Thiols

The following protocol is an adaptation for protein determination in the presence of reducing agents such as DTT (dithiothreitol) and BME (2-mercaptoethanol). This adaptation will effectively neutralize up to 50 mM BME or 5 mM DTT. Materials Req...

Researchers write protein nanoarrays using a fountain pen and electric fields

... team included directly writing organic and inorganic materials on a number of different substrates. These included suspensions of gold nanoparticles, thiols and DNA patterned on metallic- and silicon-based substrates. In the case of protein deposition, the team found that by applying an electrical field...

ESA Receives 2008 Frost & Sullivan North American Technology Innovation Award for Development of Integrated Metabolomics Systems

...ulochem(R) III system equipped with ESA's new Boron Doped Diamond cell enables life-science researchers to measure difficult-to-analyze molecules like thiols and disulfides, which are critical to a variety of biologically and clinically important processes -- from oxidative metabolism, to cardiovascular and...

Quantification of the Purinergic Receptor P2X3 using ICAT and Orthogonal 2D LC-MS/MS with an Ion Trap Mass Spectrometer

...ortant analytical challenge. As expected, cysteine thiols that occur in highly hydrophobic environm...is hinges on the ability to efficiently label free thiols (i.e., on cysteines localized in extra-ce...ure 3 confirmed the accessibility of free thiols for labeling by ICAT. This result was also ...
Thiols in Biological Products

Dithio-bis(2-nitrobenzoic acid) BioChemica from AppliChem GmbH

Description:... The chromogenic reactant should not interfere with the enzymic reaction. 5,5'-Dithiobis-2-nitrobenzoic acid (DTNB, Ellman's reagent) reacts with free thiols in an exchange reaction that produces the yellow 4-nitrothiolate anion. For the assay of acetylcholine esterase (E.C. 3.1.1.7), acetylthiocholine iodi...
Company:AppliChem GmbH

IMPACT-CN System from New England Biolabs

Description:...f the intein and the chitin binding domain (5), to either the C-terminus (pTYB1,2) or N-terminus (pTYB11,12) of the target protein. In the presence of thiols such as DTT, -mercaptoethanol or cysteine, the intein undergoes specific self-cleavage which releases the target protein from the chitin-bound intein ...
Company:New England Biolabs

Total Antioxidant Power Colorimetric Assay Kit from Neogen Corporation

Description:...th intra- and extracellular). Lipid soluble antioxidants (most importantly vitamin E) and water-soluble antioxidants (uric acid, vitamin C, bilirubin, thiols and gluthathione) are involved in these processes. Given the multiplicity of antioxidant pathways, their centrality in the prevention of oxidant stres...
Company:Neogen Corporation
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