Verenium and Add Food Service GmbH Announce Commercial Distribution Partnership for Fuelzyme(R) and Veretase(TM) Enzymes

...is effective over an exceptionally wide temperature and pH range allowing ethanol producers greater operational flexibility. Fuelzyme is an extremely thermostable alpha amylase with a unique mode of action for starch breakdown enabling ethanol producers to achieve significant enzyme dose reductions resulting in ...

Enigma Diagnostics Announces Patent On Real-Time Monitoring of Amplification Reactions Upheld In Entirety Following Opposition From BD at European Patent Office

...tfolio of intellectual property exclusively licensed to Enigma Diagnostics by the Secretary of State for Defence. The technology involves the use of a thermostable endonuclease to cut a probe that recognises a specific sequence in the target amplification product. The system may be applied to a wide variety of mo...

Thermostable in Medical Products

ThermalSeal (sterile)

Description:... the market, it was developed with the assistance of a major thermal cycler manufacturer for PCR applications. ThermalSeal prevents vapor loss and is thermostable and functional from -40C to +125C at 75% humidity. It can be used instead of plate covers to prevent sample evaporation and contamination in sensitiv...
Company:MIDSCI

ThermalSeal (non-sterile)

Description:... the market, it was developed with the assistance of a major thermal cycler manufacturer for PCR applications. ThermalSeal prevents vapor loss and is thermostable and functional from -40C to +125C at 75% humidity. It can be used instead of plate covers to prevent sample evaporation and contamination in sensitiv...
Company:MIDSCI

Thermostable in Biological News

Earth's original ancestor was LUCA, not Adam nor Eve

...development of life on Earth. From RNA to DNA: Proof of evolution "It is only in a subsequent step that LUCA's descendants discovered the more thermostable DNA molecule, which they independently acquired (presumably from viruses), and used to replace the old and fragile RNA vehicle. This invention allowed...

DOE JGI finishes 100th microbial genome

...rs and other unique sources has resulted in the discovery of a new class of thermostable DNA polymerases and unique thermostable cellulase and hemicellulase enzymes. Without the DOE JGI these valuable mol...

Thermostable in Biological Technology

QIAGEN LongRange PCR and 2Step RT-PCR Kits

... The QIAGEN LongRange PCR Kit contains all of the components required for the amplification of long PCR fragments. A unique blend of thermostable DNA polymerases, with enhanced processivity, allows the amplification of full-length PCR products. The enzyme mix provides high ...

Recombinant HIV Reverse Transcriptase

... More thermostable than AMV RT and M-MLV RT Excellent target to eval...d of a 66 kDa and a 51 kDa subunit. HIV RT is More thermostable than M-MLV RT and AMV RT HIV RT performs as ...

DNA-free A NEW Method to Remove DNA

...ctivated without loss of RNA. However, Mg 2+ is needed for enzymatic activity of both the reverse transcriptase and the thermostable DNA polymerase. Thus the chelation capacity of the EDTA must be saturated with additional ions prior to subsequent enzym...

PCR Polymerases Application Profiles

... Taq DNA polymerase is still the most commonly used thermostable polymerase for PCR today. However, Taq polymerase has serious limitations like the lack of an error-correcting activity and can ...

Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System

...FiPLUS Enzyme Blend the product of choice for the simultaneous amplification of a larger number of targets. Various thermostable DNA polymerases as individual enzymes or blends offer good yield or accuracy. However, the Expand HiFiPLUS Enzyme Blend is the only formulation ...

RT-PCR procedures

...on is then transferred to another tube (containing thermostable DNA polymerase, DNA polymerase buffer, ...mized PCR buffer (without Mg 2+ ions), a thermostable DNA polymerase, and specific primers are ...nation of reverse transcriptase and thermostable DNA polymerase, two-step RT-PCR can...

PCR reaction components

...in blends of Taq DNA polymerase and a thermostable polymerase with proofreading activity). ... For most assays, the optimum amount of thermostable DNA polymerase (or a blend of polymerases...on buffer supplied with the corresponding thermostable DNA polymerase (pH 8.39.0) will give optimal resul...

PCR principles

... Replacing a single polymerase with a blend of a thermostable polymerase (Taq DNA Polymerase) and a proofreading polymerase (Pwo DNA Polymerase) made PCR an indispensable tool in the...

High Fidelity PCR Master

...ents (buffers, nucleotides and enzymes) needed for PCR. The mix contains a unique enzyme blend of Taq DNA Polymerase and Pwo DNA Polymerase[a thermostable enzyme with a proofreading (3'5' exonuclease) activity]. Due to the inherent 3'5' exonuclease proofreading activity of Tgo DNA Polym...

Expand 20 kbPLUS PCR System

...que enzyme blend containing Taq and Pwo DNA Polymerase and another thermostable enzyme. The Expand 20 kb PLUS PCR System also includes an optimiz...ystem Increases the reliability of long PCR, because the extra thermostable enzyme improves overall performance of the enzyme blend ...

Thermostable in Biological Products

Expand 20 kb ^PLUS PCR System; Thermostable DNA polymerase mixture from Roche Applied Science

Description: Unique enzyme blend containing Taq and Pwo DNA Polymerase and another thermostable enzyme. The Expand 20 kbPLUS PCR System also includes an optimized reaction buffer, MgCl2 solution, and a human control DNA (template DNA plus beta-globin primers)....
Company:Roche Applied Science

Expand High Fidelity ^PLUS PCR System; Thermostable DNA polymerase mixture from Roche Applied Science

Description: Roche Applied Science, which pioneered the blending of thermostable DNA polymerases with the Expand High Fidelity and Expand Long Template PCR Systems, now introduces the Expand High FidelityPLUS PCR System the first product of a family of next-generation PCR Blends. This new blend combines Taq D...
Company:Roche Applied Science

Tfi DNA Ligase, 2,000U, thermostable from Bioneer, Inc.

Description: Tfi DNA Ligase catalyzes the formation of a phosphodiester bonds between adjacent 3-hydroxyl and 5-phosphate termini in nicked duplex DNA molecules or between oligonucleotides which are in duplex with a complementary strand at 45 - 65?. Tfi DNA Ligase is stable and active at much higher temperature...
Company:Bioneer, Inc.

Blunt-ended PCR Cloning Kit / pMOSBlue Competent Cells from GE Healthcare, formerly Amersham Biosciences

Description:...ing approaches by offering consistently better signal-to-noise ratios and as many as 25-fold more positive clones.Amplicons generated by proof-reading thermostable polymerases are fully compatible.High efficiency cloning, no requirement for time-consuming restriction digests and blue/white screening.Linearized at...
Company:GE Healthcare, formerly Amersham Biosciences

Zero Blunt TOPO PCR Cloning Kit with One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli from Invitrogen

Description:...t TOPO PCR Cloning Kit offers the fastest and easiest method for high-efficiency (>=95%) cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kit includes the linearized and topoisomerase I-activated pCR-Blunt II-TOPO vector for 5-minute bench-top ligations without ligase (1...
Company:Invitrogen

Zero Blunt TOPO Kit for Sequencing with One Shot Mach1 T1 Phage-Resistant Chemically Competent E. coli from Invitrogen

Description:... for direct ligation of Taq-amplified PCR products. The pCR4Blunt-TOPO vector provides blunt ends for cloning PCR products amplified with proofreading thermostable polymerases. Each vector is supplied linearized and topoisomerase I-activated enabling 5-minute bench-top ligation yielding recombinants (1). Both vec...
Company:Invitrogen

Zero Blunt TOPO PCR Cloning Kit with One Shot TOP10 Chemically Competent E. coli from Invitrogen

Description:... offers the fastest and easiest method for high-efficiency (greater than or equal to95%) cloning of blunt-end PCR products amplified with proofreading thermostable polymerases. The kit includes the linearized and topoisom...
Company:Invitrogen

GeneRacer 3' Nested Primer from Invitrogen

Description:...erScript II RT has been inactivated to avoid cleaving mRNA during cDNA synthesis. This increases the size and yield of cDNA. SuperScript II RT is more thermostable than wild-type RTS. This enables reverse transcription at higher temperatures, relaxing secondary structure of complex templates, and allowing cDNA sy...
Company:Invitrogen

DNA Sequencing from Dominion Pharmakine S.L.

Description:... experimental process of determining the nucleotide sequence of a DNA region. The automated DNA Sequencing is based on Sangers enzymatic method with a thermostable DNA polymerase, which allows cycle sequencing reactions. The resulting fluorescent products are electrophoresed and detected in a capillary instrument...
Company:Dominion Pharmakine S.L.

NovaTaq DNA Polymerase from Novagen

Description:... NovaTaq DNA Polymerase is a premium quality recombinant form of Thermus aquaticus DNA polymerase. This thermostable enzyme is suitable for a wide range of PCR applications. To ensure the highest purity and reproducible performance, each preparation is extensively te...
Company:Novagen

Thermostable in Biological Definition

Polymerase chain reaction

...temperature of over 110C. The DNA-Polymerase taken from these organisms is thermostable (stable at high temperatures) and, when used in PCR, did not break down wh...ng a given DNA strand could be simplified and automated. One of the first thermostable DNA-Polymerases was obtained from Thermus aquaticus and called Taq. T...

Thermostable in Biological Dictionary

Taq polymerase

... Taq polymerase is a thermostable DNA polymerase named after the thermophilic bact... <em> Taq </em> DNA Polymerase is a thermostable DNA polymerase that possesses a 5' ... When shou... <I> Taq </I> DNA Polymerase is a thermostable DNA polymerase that possesses a 5' ... When shou...

Polymerase

...ecular biology by which a small fragment of deoxyribonucleic acid DNA can be rapidly cloned, or... <I>Taq</I> DNA Polymerase is a thermostable DNA polymerase that possesses a 5'->3' polymerase activity and a double-strand specific 5'->3' exonuclease activity. ... DyNAzyme EXT D...

Ligase

...revisiae (Baker's yeast) ... DNA ligase , conserved site ... EPICENTRE develops and manufactures reagents and kits for genomics, proteomics, ... thermostable RNA Ligase . rGka DNA Polymerase. RNA 5' Polyphosphatase ... USB Corporation is an established supplier of reliable biochemicals and molecular bi...

Endonuclease

...cosylase and an AP-lyase. ... Endonuclease VIII Reaction Buffer (10X) Enzyme Properties. Heat ... <em>Tth</em> Endonuclease IV is a thermostable apurinic/apyrimidinic (AP) endonuclease from <em>Thermus thermophilus</em>. <em>Tth</em> Endo IV will hydrolyze an AP ... ...

DNA ligase

... ligase activity by arsenite: a possible mechanism of its ... EPICENTRE develops and manufactures reagents and kits for genomics, proteomics, ... thermostable RNA Ligase . rGka DNA Polymerase. RNA 5' Polyphosphatase ... Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate ...

Cloning

... " ... This chapter of the Promega Protocols and Applications Guide is a resource for the most current information on Cloning . ... for Cloning . thermostable DNA ... Cloning , process of creating an exact copy of a single gene, cell, or organism. ... Cloning - Wikipedia, the free encyclopedia ... I...

AP endonuclease

...can replace Apn1 ... Robust class II AP endonuclease was present in both preparations, at ... <em>Tth</em> Endonuclease IV is a thermostable apurinic/apyrimidinic ( AP ) endonuclease from <em>Thermus thermophilus</em>. <em>Tth</em> Endo IV will hydrolyze an AP .....