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Thawed in Medical News

A Growing Number of Brits Cross the Atlantic for Donor Egg IVF Treatment at Shady Grove Fertility Center

...such as hormone injections to prepare the woman's uterus for pregnancy -- while SGFC harvests the donor's eggs and fertilizes them in vitro with the thawed sperm. Only then does the couple return to Maryland for embryo transfer. This streamlined, trans-Atlantic system results in pregnancy success rates ...

Most IVF Patients Under 35 Should Have Single Embryo Transfer, According to Latest SART Data

...n a frozen state for future use," noted Dr. Feinberg. "And we believe that the single embryo transfer approach is also highly effective for thawed embryos, as shown with our pregnancy and implantation rates utilizing these embryos." "Interestingly, we have found that the majority of...

Women Unhappy With Disposal Options After Infertility Treatment

...ed said they were very unlikely to choose to allow the frozen embryos to be thawed and thrown away, used by other women, given to researchers, or frozen "fore...at scientists can do with them on the stem-cell research front. They can be thawed and discarded, although that seems akin to abortion to some people. In t...

Duska Therapeutics Acquires Technology for ATPotent Sperm Motility Program

...frequency of hyperactivated sperm. The improved motility parameters were also observed in human sperm derived from males with asthenozoospermia and in thawed cryopreserved human sperm. Results from these recent studies support earlier observations from a non-Duska sponsored European study in which increas...

Stored Ovarian Tissue Needs Testing Before Re-Implantation

...n about to receive chemotherapy for hematological cancers such as Hodgkin lymphoma, non-Hodgkin lymphoma and leukemia. Over the years, the strips were thawed and checked for cancer cells using the most modern methods available at the time. The results were compared with the same tests carried out on the pat...

Cancer could return unless stored ovarian tissue undergoes adequate testing before re-implantation

...hese extra strips for future checks for the presence of cancer cells, using the most modern methods that would be available at the time the tissue was thawed and prepared for transplantation. When the ovarian tissue was thawed, they used several different methods to detect minimal residual disease: H...

Coming soon: Cell therapies for diabetes, cancer?

...er of islets decreased. Techniques for freezing human islet cells from non-heart beating donors are being developed with an emphasis on evaluating the thawed islet morphology, possible contamination and quality of endocrine function. Cancer stem cells: Key to a cure? Researchers have proposed that c...

Therapy Could Save Limbs After Frostbite

...study. When severe frostbite occurs, blood vessels are affected and blood flow is blocked. But small clots can also form when the blood vessels are thawed and re-warmed, and spasm of the damaged arteries further obstructs blood flow to the smallest blood vessels, the U.S. team notes. This study includ...

Hey Boomers, Here's to Your Health!

...ective antioxidants*(Makes 4 Servings) 2 teaspoons canola oil 1 medium yellow onion, finely chopped 1 (10-ounce) package frozen chopped spinach, thawed and drained 1-1/2 cups grated Cabot 50% Reduced Fat Cheddar (about 6 ounces) 6 large egg whites 1 large egg 1/3 cup Cabot No Fat Cottage Cheese 1...

Freeze-dried tendon implants prove effective in early studies

...partment of Orthopaedics and Rehabilitation at the University of Rochester Medical Center, and a study author. It could conceivably be freeze-dried, thawed and then freeze-dried again without damaging it. It could be left on shelves at tissue banks indefinitely and then shipped long distances. To get it r...
Thawed in Medical Technology

Cordex Signs European Development Partnership for ATPotent(TM) Sperm Motility Drug

...so observed in human sperm derived from males with asthenozoospermia and in thawed cryopreserved human sperm. Results from these published studies suppor...ing sperm obtained from infertile males due to asthenozoospermia as well as thawed sperm that had been cryopreserved. About Cordex Pharma, Inc. Corde...

Freezing Kidney Cancer: Hot Treatment Should Be New Gold Standard for Destroying Small Tumors

...f ultrasound and CT. The needle, or cryoprobe, is filled with argon gas, which creates an ice ball, which rapidly freezes the tumor. The tumor is then thawed by replacing the argon with helium. The procedure consists of two freezing and one thawing cycle, seeking a frozen margin beyond the tumor edge to ens...

Research Documents Scalable Production, Survival Following Engraftment and Positive Impact on Cardiac Function in Infarcted Rats for Geron's Human Embryonic Stem Cell-Based Therapeutic for Heart Failure

...y at scales sufficient to enable both preclinical and early clinical development. Moreover, the final cardiomyocyte product could be cryopreserved and thawed with high viability. When transplanted together with survival enhancement factors directly into the myocardial infarct zone of rats, the GRNCM1 cell...
Thawed in Biological News

Single thawed embryo transfer after PGD does not affect pregnancy rates

Amsterdam, The Netherlands: Transferring just one embryo at a time to a woman's womb after embryos have undergone preimplantation genetic diagnosis (PGD) and freezing at the blastocyst stage has become a real option after researchers achieved pregnancy rates that were as good as those for blastocy...

Freezing kidney cancer: Hot treatment should be new gold standard for destroying small tumors

...f ultrasound and CT. The needle, or cryoprobe, is filled with argon gas, which creates an ice ball, which rapidly freezes the tumor. The tumor is then thawed by replacing the argon with helium. The procedure consists of two freezing and one thawing cycle, seeking a frozen margin beyond the tumor edge to ens...

Grape shapes

...upported the SigmaScan analysis," reported the researchers. The grapes used for the study were frozen at the time of harvest and did not need to be thawed or cut to be photographed. Ten grapes were photographed at one time, but more grapes could be shot and studied in a single photo in the future to furt...

UAF researchers to present at AGU press briefing

...as form or in ice-like methane hydrates, is trapped underneath the permafrost. When the permafrost thaws, the trapped methane can seep out through the thawed soil. Methane, a greenhouse gas 20 times more powerful than carbon dioxide, is thought to be an important factor in global climate change. The East...

Research around the North Pole

... Such a large amount of sediments causing a shift can only have one reason: the sediments were frozen in the ice masses of the East-Siberian mainland, thawed during an interglacial and unloaded their sediments with the melt water into the ocean. "This is a spectacular finding. Large-scale glaciations in eas...

Bad sign for global warming: Thawing permafrost holds vast carbon pool

... shrub land. Because plants take in carbon dioxide and release oxygen, it might appear they could compensate for whatever carbon is released by the thawed permafrost. But Schuur said the amount of carbon stored in the permafrost is far greater than what is found in shrubs or trees. For example, he sai...

Children born from frozen embryos weigh more and do better than those born after fresh transfer

...ilable in Spanish . Barcelona, Spain: Children born after a frozen, thawed embryo has been replaced in the womb have higher birth weight than those bo...cles of IVF/ICSI from the same egg collection. The embryos are subsequently thawed and replaced three to five days after ovulation in exactly the same way as ...

Scientists melt million-year-old ice in search of ancient microbes

...e University of Delaware and the University of California at Riverside have thawed ice estimated to be at least a million years old from above Lake Vostok, an...in Lanoil's laboratory in California, segments of a tube-like ice core were thawed under meticulous, clean lab conditions to prevent accidental contamination,...

Cryoablation -- A new treatment option for some kidney tumor patients

... The needle, or cryoprobe, is filled with argon gas, which results in rapid freezing of the tissue to temperatures of -100° C; and the tissue is then thawed by replacing the argon with helium. The procedure consists of two freezing and thawing cycles, seeking a frozen margin of approximately 5 millimeters...

Microbes compete with animals for food by making it stink

...wo days. They also baited other traps with freshly thawed menhaden, which contained relatively few microbes.... Many more animals were attracted to the freshly thawed bait than the rotten fish. "So we assumed that had...foods made from pureed forms of either the freshly thawed menhaden or the rotten bait. Researchers found tha...
Thawed in Biological Technology

EMD Serono Launches Landmark Patient Registry for Egg Freezing

...ms to enroll approximately 400 women of reproductive age over a three year period. Women participating in the Registry will have their oocytes frozen, thawed and the resulting fertilized embryos transferred. It is open to all qualified investigators across the country who are freezing and later thawing oocy...

Pioneering IVF Technique Produces Region's First Pregnancy

...d, frozen donated eggs. In May, frozen donor eggs were shipped to the Delaware-based infertility clinic. Soon after, the eggs were successfully thawed and fertilized. An ongoing pregnancy occurred following the first attempt at transferring the embryos to the patient's uterus. Dr. Ronald F. Fe...

Dual-Luciferase Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #39)

...prepared in phosphate-buffered saline (PBS) containing 1% bovine serum albumin (BSA) and frozen in aliquots at -70C until use. When needed, a vial was thawed and a 10 g/mL standard prepared by diluting 10 L of the stock with 990 L passive lysis buffer. 3. The Renilla luciferase stock (10 g/mL) was...

DNA Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #37)

...l Bulletin No. 278) werefollowed, except for minor modifications to accommodate a 96-well format. 1. The enzyme and buffer solutions were thawed and kept on ice. 2. TE buffer (10 mM Tris and 1 mM EDTA in sterile, deionized water, pH adjusted to 7.4) was sterilized by filtration through a...

ATP Quantitation in the LMax Microplate Luminometer (MaxLine Application Note #40)

...ped through the system and allowed to stand for one hour, followed by a 100 mL deionized water rinse. 2. The enzyme and buffer solutions were thawed and kept on ice. 3. The L/L reagent was reconstituted by pouring the Reconstitution Buffer into the vial, gently swirling several times and all...

Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons

...kept in a box at -80 until ready for LCM. One or two sections at a time are thawed and dehydrated for microdissection. 4.It is very important to keep...of P 33 UTP. 4. Fresh frozen sections of 6-12 m in thickness were thawed and fixed in 4% fresh, cold formaldehyde, followed by a wash in DEPC-treate...

Microgenomic Expression Profiling

... Use only new, sterile RNase-free barrier pipette tips and non-stick RNase free microcentrifuge tubes. Keep thawed RNA on ice until it is needed in the procedure. ◊ Ensure sample homogeneity for accurate nucleic acid quantitation when using the NanoDr...

Use of BRET2 luminescence cell-based assays with LEADseeker Multimodality Imaging System

...ration 1.1 Prepare reagents as recommended by the kit manufacturer. Reagents, cell extracts (three vials) and buffer (four vials) should be thawed on ice.

1.2 Preparation of DeepBlueC

1.2.1 Add 25 µl ethanol to the DeepBlueC vial (final concentration = 1 m...

Use of the HitHunter EFC Chemiluminescent Detection Kits with LEADseeker Multimodality Imaging System

...escribed in the pack leaflet.)

2. p38 MAPK method (Note: The assay was set up in triplicate and all reagents were thawed on ice. The assay was carried out at room temperature.)

2.1 Prepare all reagents as recommended by the kit manufacturer.

Performance of Ad-A-Gene Cyclic AMP Response Element-Nitroreductase (CRE-NTR): an adenoviral vector gene delivery system

...growth were detached by treatment with trypsin. Cell concentrations were adjusted to suitable levels with culture medium. Ad-A-Gene CRE-NTR vector was thawed by placing the tube on ice, added to the cell suspension, and mixed to provide the appropriate multiplicity of infection (MOI) for the assay * . The c...
Thawed in Biological Products

Captiva 96-well 20u Filter Plates from Varian, Inc.

Description:...olumns. The Captiva 10 um glass fiber and 20 um polypropylene filter plates are designed for clarifying highly particle-laden samples, such as freshly thawed plasma, preventing sample transfer problems due to pipette tip clogging....
Company:Varian, Inc.

Rabbit Serum Non-Hemolyzed, Sterile, Delipidized from Pel-Freez Biologicals

Description:...nian 8 - 10 weeks old, either sex) is refrigerated within 6 hours of collection, processed within 24 hours and frozen at -20ºC. The serum is then thawed in a cool waterbath, delipidized using a standard in-house protocol, filtered through a 0.22 micron filter, bottled, and refrozen at -20ºC....
Company:Pel-Freez Biologicals
Thawed in Biological Dictionary

Arrayed library

...2) Arrayed library clones suffer from repeated freezing and thawing. In order to replicate an arrayed library , the master copy needs to be thawed and ... ... C-compatible array library in ... Home / Mac / Development / Libraries . Report spyware. carray 0.1.3 ... for multi-dimensional a...
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